This work proposes the generation of multi-band K-band, V-band, W-band of millimeter-wave (mm-wave) and its transmission performance through the standard SMF of various lengths. The optical frequency multiplication (OFM) principle is used for multi-band signal generation by the optical modulator’s non-linear characteristic. The frequency doubling, quadrupling, and six-tupling are done for multi-band generation. The generated multi-band is modulated with downlink data at the central station and transmitted through the conventional Radio over Fiber (RoF) network. The received signal is photo-electrically converted by heterodyne mixing with the local oscillator according to the user’s requirement. The transmission performance is analyzed at the receiver end for varying lengths of SMF. The simulation analysis result shows that the RoF system holds good for multi-band transmission of 30, 60, 90 GHz for about 40 km. The power penalty for 30, 60, 90 GHz signal from B-T-B to 40 km is -3.79 dB, -4.847 dB, -6.33 dB.