Optical full Hadamard matrix multiplexing and noise effects: errata

Streeter, Lee; Cree, Michael J.; Burling-Claridge, G. Robert

doi:10.1364/ao.50.006092

Cited by 2 publications

(1 citation statement)

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“…As Hadamard patterns contain +1 s and − 1 s and DMDs are limited to values from 0 to 255, two complimentary patterns, one reflecting +1 s and it's inverse are measured and subtracted from each other to reflect the measurement as acquired by the original pattern. Using a Hadamard H‐matrix and subtracting the measurement of the respective negative component from the positive component measurement, is considered to help reduce the additive noise, photon and random noise in the overall measurement as stated in [27]. For a classical single‐pixel configuration (intensity based only), the emissions of the sample go through the structured DMD, and then are received by a single detector, like a photomultiplier tube (PMT) or a single‐photon avalanche diodes detector, which records the intensity for that specific DMD pattern.…”

Section: Sp‐mafli System Configurationmentioning

confidence: 99%

Computational macroscopic lifetime imaging and concentration unmixing of autofluorescence

Ochoa

Smith

Gao

et al. 2022

Journal of Biophotonics

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Single-pixel computational imaging can leverage highly sensitive detectors that concurrently acquire data across spectral and temporal domains. For molecular imaging, such methodology enables to collect rich intensity and lifetime multiplexed fluorescence datasets. Herein we report on the application of a single-pixel structured light-based platform for macroscopic imaging of tissue autofluorescence. The super-continuum visible excitation and hyperspectral single-pixel detection allow for parallel characterization of autofluorescence intensity and lifetime. Furthermore, we exploit a deep learning based data processing pipeline, to perform autofluorescence unmixing while yielding the autofluorophores' concentrations. The full scheme (setup and processing) is validated in silico and in vitro with clinically relevant autofluorophores flavin adenine dinucleotide, riboflavin, and protoporphyrin. The presented results demonstrate the potential of the methodology for macroscopically quantifying the intensity and lifetime of autofluorophores, with higher specificity for cases of mixed emissions, which are ubiquitous in autofluorescence and multiplexed in vivo imaging.

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Section: Sp‐mafli System Configurationmentioning

confidence: 99%