Optical fiber biosensor for the determination of low biochemical oxygen demand

Chee, Gab-Joo; Nomura, Yoko; Ikebukuro, Kazunori; Karube, Isao

doi:10.1016/s0956-5663(00)00093-2

Cited by 80 publications

(33 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar trend has been reported for macroscale biological oxygen demand sensors. Previously, some authors (5,40) showed that with immobilized Bacillus subtilis cells (on dialysis membranes) and Pseudomonas sp. cells (on cellulose nitrate membranes) there was an initial increase in the response of the sensors with increasing cell loads and that the sensor signal subsequently decreased with higher cell loads.…”

Section: Resultsmentioning

confidence: 99%

“…The general limitations of amperometric sensors (e.g., oxygen consumption, signal drift, fragility, time-consuming construction) prompted the development of optically based microbial macrobiosensors that have been used recently for measurement of biological oxygen demand (5,34). This study was the first attempt to use a newly developed optical ADOC microbiosensor that has numerous advantages compared to electrochemical sensors (e.g., easier construction and handling, no polarization, no signal drift, no electrical disturbance).…”

Section: Vol 72 2006 Measurement Of Adoc 7069mentioning

confidence: 99%

See 1 more Smart Citation

Microbiosensors for Measurement of Microbially Available Dissolved Organic Carbon: Sensor Characteristics and Preliminary Environmental Application

Köster

Gliesche

Wardenga

2006

Appl Environ Microbiol

View full text Add to dashboard Cite

Microbial respiration-based microbiosensors used for quantification of available dissolved organic carbon (ADOC) instantaneously respired by microorganisms are described. The sensing membranes contained aerobic seawater microorganisms immobilized in a polyurethane hydrogel. Molecular investigations revealed that the bacterial strain used was most closely related to Staphylococcus warneri. This strain was characterized by low substrate selectivity, which was reflected in the response to various mono-and disaccharides, short-chain fatty acids, and amino acids, as determined using Biolog microplates. Specific emphasis was placed on critically assessing biosensor functioning that was affected by preconditioning of the selected bacterial strain, chemical and geometric properties of the sensing membrane (e.g., composition, permeability, and thickness), and the distribution, biomass, and physiological state of immobilized cells, as well as the exposure conditions (e.g., temperature and nutrient supply). The sensors revealed that there was a linear response up to a glucose concentration of 500 M depending on the type, characteristics, and recent history of the sensors. The detection limit of the sensors was equivalent to about 6 to 10 M glucose. The 90% response time ranged from 1 to 5 min. Generally, the response of the biosensors became weaker with time. The shelf lives of individual sensors were up to 2 weeks. Measurements based on optical ADOC microbiosensors revealed that in photoautotrophically dominated sandy coastal sediments, the pool sizes and turnover of ADOC were regulated by the photosynthetic activity of benthic microalgae and microbial aerobic respiration. A large increase in ADOC production was observed shortly after the microphytobenthic primary production reached the maximum value at midday, whereas ADOC was consumed by microbial respiration during the night.A relatively new and challenging technique in microbial ecology is the use of whole-cell microbial biosensors (12, 14, 48) for measurement of various organic and inorganic compounds in aquatic environments. Such biosensors consist of a transducer (e.g., an electrochemical electrode or optode) (for reviews, see references 2, 4, 9) in conjunction with prokaryotic or eukaryotic microorganisms that either are immobilized in a polymeric matrix or are suspended in a reaction chamber in front of the sensor tip. Metabolic functions, such as respiration or the luminescence of cells, are used to monitor the concentration of analytes that are either substrates or inhibitors of these processes. Depending on their selectivity, biosensors enable workers to measure either a specific microbial response to a single compound (specific biosensors) or a common response to groups of environmental compounds (nonspecific biosensors). The rapid and sensitive responses of biosensors have been utilized to determine the presence of a broad spectrum of substances, especially for environmental control (e.g., biosensors for environmental contaminants [47]) and toxic substances ...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Vol 72 2006 Measurement Of Adoc 7069mentioning

confidence: 99%

Microbiosensors for Measurement of Microbially Available Dissolved Organic Carbon: Sensor Characteristics and Preliminary Environmental Application

Köster

Gliesche

Wardenga

2006

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Satisfactory coincidence with the data of standard BOD 5 method was obtained (Pang et al, 2007). The work (Chee et al, 2000) describes the BOD sensor based on an oxygen optrode and Pseudomonas putida cells. The sensor detected BOD in the range of 1-10 mg/l and was insensitive to chlorides and heavy metal ions.…”

Section: Bod Detectionmentioning

confidence: 63%

The Microbial Cell Based Biosensors

Решетилов¹,

Iliasov²,

Reshetilova³

2010

Intelligent and Biosensors

View full text Add to dashboard Cite

“…An optical fiber biosensor was developed for the evaluation of low BOD values in river waters by Chee et al [131]. The immobilized Pseudomonas putida bacterium membrane was placed on the top of an optode, which was linked to a photodiode that detected fluorescence signal.…”

Section: Biochemical Oxygen Demandmentioning

confidence: 99%

Biosensors for environmental applications: Future development trends

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Pure and Applied Chemistry

197

View full text Add to dashboard Cite

Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. In this context, bioelectronics, nanotechnology, miniaturization, and especially biotechnology seem to be growing areas that will have a marked influence on the development of new biosensing strategies in the next future.

show abstract

Optical fiber biosensor for the determination of low biochemical oxygen demand

Cited by 80 publications

References 28 publications

Microbiosensors for Measurement of Microbially Available Dissolved Organic Carbon: Sensor Characteristics and Preliminary Environmental Application

Microbiosensors for Measurement of Microbially Available Dissolved Organic Carbon: Sensor Characteristics and Preliminary Environmental Application

The Microbial Cell Based Biosensors

Biosensors for environmental applications: Future development trends

Contact Info

Product

Resources

About