Optical Design for Spatial-Spectral Volume Holographic Imaging System

Gelsinger, Paul J.; Lo, Yuan Hung; Barbastathis, George; Kostuk, Raymond K.; Barton, Jennifer K.

doi:10.1364/biomed.2008.bmf2

Cited by 9 publications

(13 citation statements)

References 9 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relay was configured for 1:1 imaging to image the pupil of the microscope objective at the hologram plane, which allows the hologram to be the limiting aperture of the system and reduces beam overfill issues. 18 The holograms used in the VHI system must provide high diffraction efficiency in a single diffraction order with high angular and wavelength selectivity. These characteristics can Fig.…”

Section: Handle Opticsmentioning

confidence: 99%

“…[17][18][19][20][21][22][23] The number of simultaneous depth views is determined by the number of gratings being multiplexed into the holographic element, and reports of 2 to 5 depths have been reported using the multiplexing techniques. 24 As an optical system that is capable of concurrent visualization of surface and subsurface tissue structures, the development of endoscopic VHI systems can assist in reducing the number of unnecessary surgical procedures, such as prophylactic oophorectomies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Volume holographic imaging endoscopic design and construction techniques

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract. A reflectance volume holographic imaging (VHI) endoscope has been designed for simultaneous in vivo imaging of surface and subsurface tissue structures. Prior utilization of VHI systems has been limited to ex vivo tissue imaging. The VHI system presented in this work is designed for laparoscopic use. It consists of a probe section that relays light from the tissue sample to a handheld unit that contains the VHI microscope. The probe section is constructed from gradient index (GRIN) lenses that form a 1:1 relay for image collection. The probe has an outer diameter of 3.8 mm and is capable of achieving 228.1 lp∕mm resolution with 660-nm Kohler illumination. The handheld optical section operates with a magnification of 13.9 and a field of view of 390 μm × 244 μm. System performance is assessed through imaging of 1951 USAF resolution targets and soft tissue samples. The system has also passed sterilization procedures required for surgical use and has been used in two laparoscopic surgical procedures.

show abstract

Section: Handle Opticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Volume holographic imaging endoscopic design and construction techniques

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, if light with a specific wavelength is not spatially separated along the x axis in object space, the optical sectioning performance degrades due to multiple combinations of wavelength and angle that are Bragg matched within the volume object. It has been shown that for broadband illumination of an object, the out-of-focus object points are not completely rejected by the volume hologram [7][8][9][10][11][12]. There are many combinations of wavelengths and angles within the spectral bandwidth of the source and the FOV that match the Bragg condition of the hologram and are therefore diffracted.…”

Section: Principles Of Operation and Backgroundmentioning

confidence: 99%

“…As an alternative, volume holographic imaging (VHI) has been shown to provide images from multiple depths within biological tissue samples without the need for scanning [1,2]. The VHI system (VHIS) can image multiple depths simultaneously with bright-field illumination using a light-emitting diode (LED) source [3][4][5][6][7][8][9][10]. In these systems, however, it has also been shown that the VHIS produces less-than-optimal depth resolution due to the wavelength-position degeneracy [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Dual-grating confocal-rainbow volume holographic imaging system designs for high depth resolution

et al. 2012

View full text Add to dashboard Cite

Confocal microscopy rejects out-of-focus light from the object by scanning a pinhole through the image and reconstructing the image point by point. Volume holographic imaging systems with bright-field illumination have been proposed as an alternative to conventional confocal-type microscopes that does not require scanning of a pinhole or a slit. However, due to wavelength-position degeneracy of the hologram, the high Bragg selectivity of the volume hologram is not utilized and system performance is not optimized. Confocal-rainbow illumination has been proposed as a means to remove the degeneracy and improve optical sectioning in these systems. In prior work, two versions of this system were illustrated: the first version had a separate illumination and imaging grating and the second used a single grating to disperse the incident light and to separate wavelengths in the imaging path. The initial illustration of the dual-grating system has limited depth resolution due to the low selectivity of the illumination grating. The initial illustration of the single-grating system has high depth resolution but does not allow optimization of the illumination path and requires high optical quality of the holographic filters. In this paper we consider the design and tolerance requirements of the dual-grating system for high depth resolution and demonstrate the results with an experimental system. An experimental system with two 1.8 mm thick planar holograms achieved a depth resolution of 7 μm with a field of view of 1.9 mm and a hologram dispersion matching tolerance of ±0.008°.

show abstract

“…[27][28][29] The hologram is recorded by exposing a photosensitive polymer, 1,9-phenanthrenequinone-doped poly(methyl methacrylate) (PQ-PMMA), to an optical interference pattern formed with coherent laser illumination. To multiplex, a separate hologram is recorded into the same region of the element that will select light from a separate depth within the tissue sample.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

et al. 2014

Self Cite

View full text Add to dashboard Cite

Abstract. Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Optical Design for Spatial-Spectral Volume Holographic Imaging System

Cited by 9 publications

References 9 publications

Volume holographic imaging endoscopic design and construction techniques

Volume holographic imaging endoscopic design and construction techniques

Dual-grating confocal-rainbow volume holographic imaging system designs for high depth resolution

Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Contact Info

Product

Resources

About