Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Abstract

Abstract. Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

A review of snapshot multidimensional optical imaging: Measuring photon tags in parallel

“…After passing through the volume hologram, the diffracted light is collected by a lens and imaged by an FPA. VHI has been implemented in applications such as endoscopy [137, 138] and microscopy [139, 140]. Despite the snapshot advantage, the number of depth layers that can be simultaneously imaged by VHI is extremely restricted (≤5) [133].…”

Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection

“…Optical techniques can often be miniaturized for endoscopy, are robust and are relatively inexpensive. Optical coherence tomography (OCT) 8 , fluorescence confocal imaging 9 , and volume holographic imaging 10,11 , among other techniques, have been implemented into laparoscopic endoscopes for in vivo imaging. Results have been promising, but laparoscopic procedures are too invasive to be considered a regularly minimally invasive screening or surveillance method.…”

Volume holographic imaging endoscopic design and construction techniques

“…38 The preliminary design of the handle started with a simplified version of the benchtop model. 25 Weight was minimized by a selective choice of components, while keeping pupil matching requirements. An optical model of the simplified system configuration is shown in Fig.…”

“…A benchtop version of the VHI has been shown to be a beneficial tool for ex vivo imaging evaluation of precancerous and cancerous tissues using reflectance and fluorescence imaging modalities. 25 The contribution of this paper is the design, development, and characterization of a reflectance VHI endoscope system approved for in vivo clinical use.…”