Optical clearing of human dura mater by glucose solutions

Genina, Elina A.; Bashkatov, Alexey N.; Tuchin, Valery V.

doi:10.18287/jbpe17.03.010309

Cited by 19 publications

(11 citation statements)

References 32 publications

(37 reference statements)

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“…3, there is no unique filter configuration with the equally good results for all of the sample concentrations. We could notice that the measurement errors do not depend monotonically on scatterer concentration, which could be due to nonmonotonical changes of the scattering coefficient of turbid media with increased volume fraction of colloidal particles, 62,63 as well as due to complex dependence of the Mie scattering crosssection of microinclusions on the effective refractive index of colloidal suspension. 31 Moreover, when small concentrations of nanoparticles produce weak noise, σ STD has insignificant variations and all considered filter realizations have the similar effect, in contrast to the high concentration with large σ STD variations.…”

Section: Choosing the Wavelet-domain Filter And Application For Oct Imentioning

confidence: 94%

Nanoparticle-enabled experimentally trained wavelet-domain denoising method for optical coherence tomography

et al. 2018

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We present the nanoparticle-enabled experimentally trained wavelet-domain denoising method for optical coherence tomography (OCT). It employs an experimental training algorithm based on imaging of a test-object, made of the colloidal suspension of the monodisperse nanoparticles and contains the microscale inclusions. The geometry and the scattering properties of the test-object are known a priori allowing us to set the criteria for the training algorithm. Using a wide set of the wavelet kernels and the wavelet-domain filtration approaches, the appropriate filter is constructed based on the test-object imaging. We apply the proposed approach and chose an efficient wavelet denoising procedure by considering the combinations of the decomposition basis from five wavelet families with eight types of the filtration threshold. We demonstrate applicability of the wavelet-filtering for the in vitro OCT image of human brain meningioma. The observed results prove high efficiency of the proposed OCT image denoising technique.

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Section: Choosing the Wavelet-domain Filter And Application For Oct Imentioning

confidence: 94%

Nanoparticle-enabled experimentally trained wavelet-domain denoising method for optical coherence tomography

et al. 2018

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“…to suppress light scattering. This can be achieved either by using physical methods (such as tissue compression) or by physicochemical methods (such as refractive index matching or the removal of scatterers with the optical clearing agents (OCAs)) basing on tissue optical clearing (OC) protocols [25][26][27][28][29][30][31].…”

Section: Ex Vivo and In Vivo Tissue Optical Clearing Methodsmentioning

confidence: 99%

“…OC methods based on aqueous OCAs were developed to try to preserve endogenous fluorescence and tissue structure. An aqueous solution of glycerol (70-80% of glycerol) is often used for OC of skin and many other tissues [25][26][27][28][29][30][31].…”

Section: Ex Vivo and In Vivo Tissue Optical Clearing Methodsmentioning

confidence: 99%

“…The thickness of DM varies from 0.3 to 0.9 mm depending on the age. At present, different hyperosmotic liquids are used as the OCAs, e.g., the solutions of glycerol, propylene glycol, some radiopaque substances (Trazograph, Verographin, Omnipaque), etc [25,26,30,31]. For their clinical application, one should consider both their biocompatibility and their diffusion rate in the tissues.…”

Section: Optical Clearing Of Human Dura Matermentioning

confidence: 99%

“…For their clinical application, one should consider both their biocompatibility and their diffusion rate in the tissues. One of the promising candidates for the role of OCA is the aqueous glucose solution that is allowed for clinical use, possesses sufficiently high refractive index [30].…”

Section: Optical Clearing Of Human Dura Matermentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in the Laser Radiation Transport through the Head Tissues of Humans and Animals – A Review

Sabeeh

Tuchin

2020

J-BPE

Self Cite

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Modern studies of the penetration of light into biological tissues is becoming very important in various medical applications. This is an important factor for determining the optical dose in many diagnostic and therapeutic procedures. The absorption and scattering properties of the tissue under study determine how deeply the light will penetrate into the tissue. However, these optical properties are highly dependent on the wavelength of the light source and tissue condition. This overview paper analyzes the transmission of light through different areas of human and animal head tissues, and the optimal laser wavelength and power density required to reach different parts of the brain are determined using lasers with different wavelengths by comparing the distribution of fluence, penetration depth and the mechanism of interaction between laser light and head tissues. The power variation in different regions of the head is presented, as estimated using Monte Carlo (MC) simulations. Data are analyzed for the absorption and scattering coefficients of the head tissue layers (scalp, skull, brain), calculated using integrating sphere measurements and inverse problem solving algorithms such as inverse MC (IMC) and adding-doubling (IAD). This study not only offered a quantitative comparison between wavelengths in terms of light transmission efficiency, but also anticipated the exciting opportunity for online, accurate and visible optimization of LLLT lighting parameters.

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Ex vivo confocal Raman microspectroscopy of porcinedura matersupported by optical clearing

Jaafar

Holomb

Sdobnov

et al. 2021

Journal of Biophotonics

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The effect of tissue optical clearing (TOC) to increase the probing depth and observe in‐depth structure of the ex vivo porcine dura mater was studied by confocal Raman microspectroscopy (CRM). Raman intensities were significantly increased at the depth of 250 μm for all collagen bands after treatment with glycerol. The influence of glycerol on collagen hydration was also investigated. The results indicate that the process of TOC can be divided into three main steps. The first one is a fast process of tissue dehydration accompanied by collagen shrinkage while the second relatively slow process is related to the glycerol penetration into the interfibrillar space of collagen combined with swelling of tissue. The third step is collagen dissociation caused by the high concentration of glycerol. To the best of our knowledge, this study is the first example to introduce the TOC technique in assisting CRM of ex vivo dura mater in‐depth probing.

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Optical clearing of human dura mater by glucose solutions

Cited by 19 publications

References 32 publications

Nanoparticle-enabled experimentally trained wavelet-domain denoising method for optical coherence tomography

Nanoparticle-enabled experimentally trained wavelet-domain denoising method for optical coherence tomography

Recent Advances in the Laser Radiation Transport through the Head Tissues of Humans and Animals – A Review

Ex vivo confocal Raman microspectroscopy of porcinedura matersupported by optical clearing

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