The effect of tissue optical clearing (TOC) to increase the probing depth and observe in‐depth structure of the ex vivo porcine dura mater was studied by confocal Raman microspectroscopy (CRM). Raman intensities were significantly increased at the depth of 250 μm for all collagen bands after treatment with glycerol. The influence of glycerol on collagen hydration was also investigated. The results indicate that the process of TOC can be divided into three main steps. The first one is a fast process of tissue dehydration accompanied by collagen shrinkage while the second relatively slow process is related to the glycerol penetration into the interfibrillar space of collagen combined with swelling of tissue. The third step is collagen dissociation caused by the high concentration of glycerol. To the best of our knowledge, this study is the first example to introduce the TOC technique in assisting CRM of ex vivo dura mater in‐depth probing.
Confocal Raman microspectroscopy (CRM) with 633- and 785-nm excitation wavelengths combined with optical clearing (OC) technique was used for ex-vivo study of porcine skin in the Raman fingerprint region. The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol, distilled water and chemical penetration enhancer dimethyl sulfoxide (DMSO) during 30[Formula: see text]min and 60[Formula: see text]min of treatment. It was shown that the combined use of the optical clearing technique and CRM at 633[Formula: see text]nm allowed one to preserve the high probing depth, signal-to-noise ratio and spectral resolution simultaneously. Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency. Also, an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents. As a result of optical clearing, the detection of the amide-III spectral region indicating well-distinguishable structural differences between the type-I and type-IV collagens has been improved.
Well logs data was analyzed and used to identify reservoir properties. The Mishrif Formation was sudivided into six units (MA, MB1, MB2, MC1, MC2 and MC3) with different potential characteristics. By using the MID method and M-N cross plots to determine the rock and mineral composition of the Mishrif Formation in the X oil field, it was found through the profiles that the Mishrif Formation consists largely of limestone, little dolomite and shale. Based on the CPI results of the two wells (XA and XB)it was noted that the units BM1, BM2 and MC1 reservoir units have good petrophysical properties as they had good porosity ranging from 0.1 to 0.19 with low values of water saturation (0.3-0.79) and shale volume (0.0295-0.070) respectively). Due to these characteristics these units are considered oil-bearing areas are among the most important units in the Mishrif Formation in the X field. while the other units (MA, MC2 and MC3) are considered non-reservoir units due to It has poor petrophysical properties.
Dura mater (DM) is a connective tissue with dense collagen, which is a protective membrane surrounding the human brain. The optical clearing (OC) method was used to make DM more transparent, thereby allowing to increase in-depth investigation by confocal Raman micro-spectroscopy and estimate the diffusivity of 50% glycerol and water migration. Glycerol concentration was obtained, and the diffusion coefficient was calculated, which ranged from 9.6 × 10−6 to 3.0 × 10−5 cm2/s. Collagen-related Raman band intensities were significantly increased for all depths from 50 to 200 µm after treatment. In addition, the changes in water content during OC showed that 50% glycerol induces tissue dehydration. Weakly and strongly bound water types were found to be most concentrated, playing a major role in the glycerol-induced water flux and OC. Results show that OC is an efficient method for controlling the DM optical properties, thereby enhancing the in-depth probing for laser therapy and diagnostics of the brain. DM is a comparable to various collagen-containing tissues and organs, such as sclera of eyes and skin dermis.
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