This work reports the synthesis of ordered and vertically aligned iron oxide nanowires for photoelectrochemical (PEC) water oxidation. The nanowires exhibited promising PEC activity for water oxidation with saturated photocurrents of ~0.8 mA cm-2 at 1.23 V vs RHE. Various factors inevitably affect their photochemical activity such as crystallinity, morphology, compositional gradient, and surface states. They were studied with HRTEM, EELS and Raman shift techniques. The nanowires had complex compositional and morphological structures at the nano and atomic scales. The nanowires annealed at 350 ⁰C had an outer shell dominated by Fe 3+ cations, while the core had mixed oxidation Page 2 of 44 ACS Paragon Plus Environment ACS Applied Energy Materials 3 states of iron cations (+2 and +3). In contrast, nanowires annealed at 450 ⁰C are fully oxidized with Fe 3+ cations only and were found to be more active. At the same time, we observed anisotropic compositional gradients of nickel cations inside of the iron oxide, originating from the nickel support film. Our work shows that the methodology used can affect the composition of the surface and near surface of the grown nanowires. It therefore points out the importance of a detailed analysis, in order to obtain a realistic structureactivity relationship in photo-electro-catalysis.