Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of Chlamydomonas reinhardtii

Giardi, Maria Teresa; Scognamiglio, Viviana; Rea, Giuseppina; Rodio, Giuseppe; Antonacci, Amina; Lambreva, Maya D.; Pezzotti, Gianni; Johanningmeier, Udo

doi:10.1016/j.bios.2009.07.003

Cited by 68 publications

(34 citation statements)

References 30 publications

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“…[6][7][8][9] The unusual AC capacity of the cells described above represents an additional property that can be exploited for applications, particularly if the forward and reverse current components were to be made fully symmetrical. The most obvious is as a direct source of light-generated AC, something that solar cells cannot normally achieve because their design limits them to producing a DC that has to be processed through an inverter.…”

Section: Resultsmentioning

confidence: 98%

“…[1][2][3] Photochemical charge separation occurs in these systems with a quantum yield (event per photon absorbed) close to unity, and there is growing interest in how reaction center proteins can be directly exploited for device applications in photovoltaics, biosensing, photodetection, fuel synthesis and biocomputing. [4][5][6][7][8][9][10][11][12][13][14][15] Key to the development of these new hybrid technologies is the effective interfacing of proteinaceous reaction centers with manmade materials.…”

Section: Introductionmentioning

confidence: 99%

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Superhydrophobic Carbon Nanotube Electrode Produces a Near‐Symmetrical Alternating Current from Photosynthetic Protein‐Based Photoelectrochemical Cells

Tan

Yan

Crouch

et al. 2013

Adv Funct Materials

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The construction of protein‐based photoelectrochemical cells that produce a variety of alternating currents in response to discontinuous illumination is reported. The photovoltaic component is a protein complex from the purple photosynthetic bacterium Rhodobacter sphaeroides which catalyses photochemical charge separation with a high quantum yield. Photoelectrochemical cells formed from this protein, a mobile redox mediator and a counter electrode formed from cobalt disilicide, titanium nitride, platinum, or multi‐walled carbon nanotubes (MWCNT) generate a direct current during continuous illumination and an alternating current with different characteristics during discontinuous illumination. In particular, the use of superhydrophobic MWCNT as the back electrode results in a near symmetrical forward and reverse current upon light on and light off, respectively. The symmetry of the AC output of these cells is correlated with the wettability of the counter electrode. Potential applications of a hybrid biological/synthetic solar cell capable of generating an approximately symmetrical alternating current are discussed.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Superhydrophobic Carbon Nanotube Electrode Produces a Near‐Symmetrical Alternating Current from Photosynthetic Protein‐Based Photoelectrochemical Cells

Tan

Yan

Crouch

et al. 2013

Adv Funct Materials

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“…Sensitive, selective, and cost-effective detection of biomolecules has widespread applications in clinical diagnostics and in industrial and environmental monitoring [1][2][3][4]. Nanomaterials, such as carbon nanotubes and nanowires, have been proven attractive for biosensing due to their unique structures and properties [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive protein sensor based on thermally-reduced graphene oxide field-effect transistor

Mao

et al. 2011

Nano Res.

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We report the fabrication of a highly sensitive field-effect transistor (FET) biosensor using thermally-reduced graphene oxide (TRGO) sheets functionalized with gold nanoparticle (NP)-antibody conjugates. Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding (Immunoglobulin G/IgG and anti-Immunoglobulin G/anti-IgG) was accomplished by FET and direct current (dc) measurements. The protein binding events induced significant changes in the resistance of the TRGO sheet, which is referred to as the sensor response. The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied, from which a correlation of the sensor response with sensor parameters was found: the sensor response was more significant with larger TRGO base resistance and higher antibody areal density. The detection limit of the novel biosensor was around the 0.2 ng/mL level, which is among the best of reported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure.

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“…Minimal media were used to select photosynthetically active colonies generated after the integration of the psbA variant produced both by random and site-directed PCR (Dauvillee et al, 2004). Selected mutants were then characterised by analysing their photosynthetic performance and the sensitivity and/or resistance to different classes of herbicides assessed (Tibuzzi et al, 2007;Rea et al, 2009;Giardi et al, 2009;Scognamiglio et al, 2009). After the characterization, the best performing mutants were immobilized on screen-printed electrodes and integrated in amperometric or potentiometric circuits.…”

Section: Relevance Of Genetic Engineering To Improve Sensitivity and mentioning

confidence: 99%

Computational Biology, Protein Engineering, and Biosensor Technology: a Close Cooperation for Herbicides Monitoring

Rea¹,

Polticelli²,

Antonacci³

et al. 2011

Herbicides, Theory and Applications

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Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of Chlamydomonas reinhardtii

Cited by 68 publications

References 30 publications

Superhydrophobic Carbon Nanotube Electrode Produces a Near‐Symmetrical Alternating Current from Photosynthetic Protein‐Based Photoelectrochemical Cells

Superhydrophobic Carbon Nanotube Electrode Produces a Near‐Symmetrical Alternating Current from Photosynthetic Protein‐Based Photoelectrochemical Cells

Highly sensitive protein sensor based on thermally-reduced graphene oxide field-effect transistor

Computational Biology, Protein Engineering, and Biosensor Technology: a Close Cooperation for Herbicides Monitoring

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