Optical biosensors based on Prussian Blue films

Abstract

Optical biosensing schemes based on enzymatically modified inorganic/organic transparent films predominately composed of Prussian Blue are demonstrated. The composite film, which is non-electrochemically deposited on a non-conducting support. is used as an optical transducer for flow-through biosensors based on hydrolases and oxidases. Urease and glucose oxidase are utilized as model enzymes. Action of the urea biosensor is based on optical pH sensitivity of Prussian Blue indicator. The glucose biosensor is acting as first-generation optical biosensor based on in situ generated Prussian White transducer for hydrogen peroxide. These simple, single-pass transmission optical biosensors exhibit sensitivity in the millimolar range of concentration. The biosensors are very stable owing to presence of a poly(pyrrolylbenzoic acid) network in the composite material. This organic polymer plays a dual role as a binding agent for inorganic material and as a functionalized support for strong covalent immobilization of enzyme molecules.

Urea potentiometric enzymatic biosensor based on charged biopolymers and electrodeposited polyaniline

“…Urease has often been used as the sensitizing material for the fabrication of urea sensors, like thermal (Xie and Danielsson, 1996;Xie et al, 1995;Fulton et al, 1980;Hauser et al, 1995), conductometric (Sheppard et al, 1996;Jdanova et al, 1996;Castillo-Ortega et al, 2005), piezoelectric (Xu et al, 1996;Yang et al, 2007;Yang et al, 2008), optical (Picioreanu et al, 2000;Koncki et al, 2001;Tsai and Doong, 2005), laser (György et al, 2010) and amperometric (Rajesh et al, 2005;Vostiar et al, 2002;Pizzariello et al, 2001;Stredansky et al, 2000) sensors. However, potentiometric 0956-5663/$ -see front matter © 2011 Elsevier B.V. All rights reserved.…”

Potentiometric Biosensor Based on the Recombinant Urease for the Control of the Urea Contrentation in Real Biological Samples

“…На сьо-годнішній день створено цілу низку уреазних біосенсорів, де в якості чутливого елементу ви-ступає фермент уреаза: амперометричних [7], потенціометричних [8], кондуктометричних [9] та оптичних [10]. Але, не зважаючи на значний розвиток біосенорів для моніторингу сечовини, дуже рідко підкреслюють недоліки пов'язані з їх практичним застосуванням.…”

pH-Dependent response of a hydrogen peroxide sensing probe

“…H 2 O 2 sensors are usually based on the electrochemical and spectroscopic techniques [11][12][13][14][15][16][17], with spectroscopic techniques being preferred for many biochemical and industrial applications because of their immunity to electromagnetic interference. On the other hand, Prussian blue (PB), ferric ferrocyanide, is widely used for making sensors and biosensors for detection and measurements of H 2 O 2 [11,18].…”