Urea potentiometric enzymatic biosensor based on charged biopolymers and electrodeposited polyaniline

Lakard, Boris; Magnin, Delphine; Deschaume, Olivier; Vanlancker, Guilhem; Glinel, Karine; Demoustier‐Champagne, Sophie; Nysten, Bernard; Jonas, Alain M.; Bertrand, Patrick; Yunus, Sami

doi:10.1016/j.bios.2011.04.009

Cited by 90 publications

(40 citation statements)

References 54 publications

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“…60 Polysaccharide LbL films containing enzymes have been prepared for biosensors and bioreactors. [61][62][63] We have studied the voltammetric response of a redox species on Au electrodes coated with polysaccharide LbL films. 64,65 Carboxy methylcellulose (CMC) and alginic acid (ALG) have been used in combination with PAH, PEI, or PDDA as the cationic counterpart.…”

Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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“…All other solutions were made from a stock solution of pH 7.0, 0.05 M phosphate buffer, which was initially prepared using distilled water. This concentration of phosphate buffer was chosen as higher concentrations are known to interfere with the biocatalytic activity of urease, whereas lower concentrations have insufficient conductivity [9]. All of the solutions were freshly prepared before each experiment.…”

Section: Chemicalsmentioning

confidence: 99%

“…Since then, the entrapment of urease within a polymer matrix has gained a lot of attention, with polypyrrole and polyaniline being the most common choice of conducting polymers [6][7][8][9][10]. An electroactive polyaniline film has been used for urea detection whereby the urease enzyme was entrapped within the electroactive polymer during electrodeposition [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…An electroactive polyaniline film has been used for urea detection whereby the urease enzyme was entrapped within the electroactive polymer during electrodeposition [9,10]. An electroactive polypyrrole with a polyion complex has been utilised as a composite film for the detection of urea, where the polyanion included PAA (polyacrylate) and PSS (polystyrene sulfonate) with PLL (polylysine) as the polycation.…”

Section: Introductionmentioning

confidence: 99%

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The development of a novel urea sensor using polypyrrole

Hamilton

Breslin

2014

Electrochimica Acta

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a b s t r a c tThe urease enzyme (Urs) was successfully incorporated into a polypyrrole film (PPy) in one simple electropolymerisation step. The films were formed using different dopant anions. The polypyrrole-urease-chloride film (PPy-Urs-Cl) was deposited from a simple chloride dopant, and the polypyrrole-urease-sulfonated-␤-cyclodextrin (PPy-Urs-SCD) was formed using a sulfonated-␤-cyclodextrin (SCD) dopant. The presence of the Urs within the polymer film was evident from the fibrous morphology, observed in the SEM micrographs, and the presence of nickel, arising from the active site of the urease enzyme. The sensing ability of the films and their enzyme-free counterparts (i.e., PPy-Cl and PPy-SCD) towards urea was investigated. The dopant anion plays an important role in the sensitivity of the polymer films towards urea. The PPy-Urs-SCD film has a superior sensitivity of 5.79 C M −1 compared to 0.76 C M −1 for the PPy-Urs-Cl polymer film. Furthermore, the negative groups on the SCD eliminate interference from common interfering compounds, such as ascorbic acid.

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“…Among these are corrosion protection [1,2], electronic devices such as diodes [3], capacitors [4] or transistors [5], electrochromic displays [6], rechargeable batteries [7,8] and solid electrolytes [9], electrostatic materials [10], drug release systems [11,12], electromechanical actuators [13], sensors [14,15] or biosensors [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Electrosynthesis and characterization of polymer films on silicon substrates for applications in micromanipulation

et al. 2012

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Aniline and p-phenylenediamine were electrochemically oxidized in aqueous solutions by potentiodynamic deposition using Cyclic Voltammetry (CV) technique Resulting polymer films electrodeposited on Si substrates were identified by infrared spectroscopy as polyaniline and poly(p-phenylenediamine) films. Cyclic voltammograms demonstrated that poly(p-phenylenediamine) films are non conductive contrary to polyaniline films which are conductive and electropolymerized through an autocatalytic mechanism. The morphological features (thickness, roughness, morphology) of the electrodeposited polymer films were determined using Profilometry, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Adhesion force properties of polymer films were also studied by means of force-distance curves obtained by Atomic Force Microscopy. The influence of some experimental parameters (potential scan speed, number of potential scans) was also studied. Thus, it was shown that the potential scan speed strongly influence both morphological features and force adhesion, when the influence of the number of potential scans was less important. Under optimal conditions, it was possible to obtain very adhesive polymer films that could be useful for the micromanipulation of Si objects.2

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Urea potentiometric enzymatic biosensor based on charged biopolymers and electrodeposited polyaniline

Cited by 90 publications

References 54 publications

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

The development of a novel urea sensor using polypyrrole

Electrosynthesis and characterization of polymer films on silicon substrates for applications in micromanipulation

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