Opsonization, Biodistribution, Cellular Uptake and Apoptosis Study of PEGylated PBCA Nanoparticle as Potential Drug Delivery Carrier

Chaudhari, Kiran; Ukawala, Mukesh; Manjappa, Arehalli S.; Kumar, Arun; Mundada, Piyush K; Mishra, Anil K.; Mathur, Rashi; Mönkkönen, Jukka; Murthy, R. S. R.

doi:10.1007/s11095-011-0510-x

Cited by 90 publications

(64 citation statements)

References 48 publications

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“…27) The mean zeta potential of PUE-PBCN was −7.72 mV, which was due to the hydroxylions and carbonyl group of polymer present on surface of nanoparticles, 28) and the result was close to the study on curcumin loaded PBCN. 29) The zeta potential which represents the charge of the particles plays an important role in the stability of nanoparticles.…”

Section: Discussionsupporting

confidence: 71%

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

Zhao

Liu

et al. 2013

Biological & Pharmaceutical Bulletin

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Puerarin (PUE) is a good candidate for treating stroke, but its low concentration in brain after administration limits its curative efficacy. The aim of the present work was to design and characterize PUE loaded poly(butylcyanoacrylate) nanoparticles (PBCN) coated with polysorbate 80 (Ps 80), and to evaluate the effect of PBCN on the permeability of PUE across the blood-brain barrier (BBB) and the effect of PUE loaded PBCN on the cerebral ischemia/reperfusion injury. PUE loaded PBCN were successfully prepared by anionic polymerization method with the mean particle size of 201.2 nm and the zeta potential of −7.72 mV. The in vitro release behavior of PUE from the nanoparticles showed a biphasic profile manner with an initial burst release followed by a sustained release. The results of pharmacokinetic and biodistribution to brain performed in mice after intravenous administration showed that the drug concentrations in blood and brain for PUE loaded PBCN were both greater than these for the free drug. Moreover, compared with free drug, the vein injection of PUE loaded PBCN exerted the better neuroprotective effect in rats with focal cerebral ischemic injury via significantly decreasing neurological deficit scores, increasing body weight, lowing brain water content, and reducing the infarct volume. The results indicated that this preparation may reduce the total dose required for the stroke therapy with concurrent reduction in dose related toxicity. All these findings suggest that PBCN could enhance the transport of PUE to brain and have a potential as a neuroprotective agent in the focal cerebral ischemic injury.

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Section: Discussionsupporting

confidence: 71%

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

Zhao

Liu

et al. 2013

Biological & Pharmaceutical Bulletin

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“…Most work to date evaluating cellular MP uptake and handling has utilized cell lines [34][35][36][37][38]. This risks chronic contamination by mycoplasma -such 'cryptic' contamination can alter cell structure, metabolism and growth, all of which can impact the interpretation of data [42].…”

Section: Discussionmentioning

confidence: 99%

Differences in Magnetic Particle Uptake by CNS Neuroglial Subclasses: Implications for Neural Tissue Engineering

et al. 2013

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Materials and Methods: MP uptake and processing were studied in rat OPCs and oligodendrocytes, using fluorescence and transmission electron microscopy, and results collated with previous data from microglia and astrocytes.Results: Significant intercellular differences were observed between glial subtypes, with microglia demonstrating the most rapid/extensive particle uptake, followed by astrocytes, with OPCs and oligodendrocytes showing significantly lower uptake. Ultrastructural analyses suggest that MPs are extensively degraded in microglia but are relatively stable in other cells. Conclusions:Our findings have implications for use of the MP platform in a range of neural tissue engineering applications such as transfection, cell labeling and direct CNS biomolecule delivery.

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“…[15][16][17][18][19][20] PEG is fixed on nanoparticle surfaces by adsorption or covalent binding. When functionalized with PEG, nanoparticles can avoid serum protein binding and achieve enhanced permeability and retention in tumor model systems.…”

Section: Introductionmentioning

confidence: 99%

Influence of polyethylene glycol coating on biodistribution and toxicity of nanoscale graphene oxide in mice after intravenous injection

Zhang

Yang

et al. 2014

IJN

100

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In this study, we assessed the in vivo behavior and toxicology of nanoscale graphene oxide (NGO) in mice after intravenous injection. The influence of a polyethylene glycol (PEG) coating on the distribution and toxicity of the NGO was also investigated. The results show that NGO is mainly retained in the liver, lung, and spleen. Retention in the lung is partially due to NGO aggregation. The PEG coating reduces the retention of NGO in the liver, lung, and spleen and promotes the clearance of NGO from these organs, but NGO and NGO-PEG are still present after 3 months. The PEG coating effectively reduces the early weight loss caused by NGO and alleviates NGO-induced acute tissue injuries, which can include damage to the liver, lung, and kidney, and chronic hepatic and lung fibrosis.

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Opsonization, Biodistribution, Cellular Uptake and Apoptosis Study of PEGylated PBCA Nanoparticle as Potential Drug Delivery Carrier

Abstract: The PEGylated PBCA formulation can work as a novel tool for drug delivery which can prevent RES uptake and prolong circulation half life.

Cited by 90 publications

References 48 publications

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

Differences in Magnetic Particle Uptake by CNS Neuroglial Subclasses: Implications for Neural Tissue Engineering

Influence of polyethylene glycol coating on biodistribution and toxicity of nanoscale graphene oxide in mice after intravenous injection

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Opsonization, Biodistribution, Cellular Uptake and Apoptosis Study of PEGylated PBCA Nanoparticle as Potential Drug Delivery Carrier

Abstract: The PEGylated PBCA formulation can work as a novel tool for drug delivery which can prevent RES uptake and prolong circulation half life.

Cited by 90 publications

References 48 publications

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

The Permeability of Puerarin Loaded Poly(butylcyanoacrylate) Nanoparticles Coated with Polysorbate 80 on the Blood–Brain Barrier and Its Protective Effect against Cerebral Ischemia/Reperfusion Injury

Differences in Magnetic Particle Uptake by CNS Neuroglial Subclasses: Implications for Neural Tissue Engineering

Influence of polyethylene glycol coating on&nbsp;biodistribution and toxicity of nanoscale graphene oxide in mice after intravenous injection

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Influence of polyethylene glycol coating on biodistribution and toxicity of nanoscale graphene oxide in mice after intravenous injection