Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating the apoptotic pathway

Jiao, Jianhua; Hong, Shanchao; Zhang, Jin; Ma, Lei; Sun, Yan; Zhang, Donghui; Shen, Bo; Zhu, Changliang

doi:10.1016/j.canlet.2012.01.035

Cited by 38 publications

(35 citation statements)

References 31 publications

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“…To date, only a few studies have reported its role in cancer. Only one article has previously reported that OPN3 might affect apoptosis of hepatoma cells by modulating the phosphorylation of Akt and Bcl2/Bax . We demonstrated that OPN3 could promote the EMT and tumor metastasis in LUAD.…”

Section: Discussionmentioning

confidence: 60%

“…Total RNA extracted from LUAD cell lines and tissues was treated with TRIzol reagent (TaKaRa, China). Primer sequences for OPN3 were referenced to the sequence listed in Jiao et al . Primer sequences (for CDH1, CDH2 , Vimentin, Slug, Snail ) and the experimental procedures were used as described in Xu et al …”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have demonstrated that OPN3 is expressed in lung bronchial epithelial cells and may be associated with asthma . It can affect the apoptosis of hepatoma cells by regulating phosphorylation of Akt and Bcl2/Bax . Previous studies have also confirmed the expression of OPN3 in normal lung tissue; however, to date there is no relevant study on its expression and function in LUAD.…”

Section: Introductionmentioning

confidence: 99%

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Expression of OPN3 in lung adenocarcinoma promotes epithelial‐mesenchymal transition and tumor metastasis

Wang

Yang

et al. 2019

Thoracic Cancer

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Background: Lung adenocarcinoma is the most common pathological lung cancer and an important cause of cancer-related death. Metastasis is a major underlying reason for poor prognosis of lung adenocarcinoma. Opsin3 (OPN3), a member of the guanine nucleotide-binding protein-coupled receptor superfamily, has been identified to affect the apoptosis of hepatoma cells by modulating the phosphorylation of Akt and Bcl2/Bax. However, the expression and role of OPN3 in lung adenocarcinoma remains unclear. Methods: Opsin3 expression in lung adenocarcinoma tissues was detected by western blot, qPCR, and immunohistochemistry. Changes in cell migration and invasion ability resulting from the change of OPN3 expression level were detected by wound healing and transwell migration assays. Changes in the markers of epithelial-mesenchymal transformation were detected by western blot and qPCR. Results: Opsin3 expression in lung adenocarcinoma tissues was higher than that in normal lung tissues. Patients with high expression of OPN3 had lower survival rates. Owing to overexpression of OPN3, the HCC827 cells showed enhanced invasion and migration ability in vitro. Upon decreasing the expression of OPN3, the invasion and migration ability of the A549 cells decreased. Conclusion: Our study demonstrated for the first time that OPN3 gene enhanced the metastasis in lung adenocarcinoma, and its overexpression promoted epithelial-mesenchymal transition. Key points: A significant finding of the study was that OPN3 acted an oncogene in promoting lung adenocarcinoma metastasis. Our study complemented the research on the expression and function of OPN3 in lung adenocarcinoma.

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Section: Discussionmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of OPN3 in lung adenocarcinoma promotes epithelial‐mesenchymal transition and tumor metastasis

Wang

Yang

et al. 2019

Thoracic Cancer

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“…Nevertheless, cOpn3 protein was localized to Purkinje cells of the cerebellum and thalamic nuclei in the chicken, similar to the expression observed in the mouse brain [6, 32]. It could be that chicken Opn3 protein mediates other roles, such as regulating apoptosis or immune modulation [45,46]. A recent study has shown that a chimeric chicken Opn3, in which the third intracellular loop was replaced with that of a jellyfish opsin, forms a blue-sensitive pigment [47].…”

Section: Discussionmentioning

confidence: 76%

Two Opsin 3-Related Proteins in the Chicken Retina and Brain: A TMT-Type Opsin 3 Is a Blue-Light Sensor in Retinal Horizontal Cells, Hypothalamus, and Cerebellum

et al. 2016

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Opsin family genes encode G protein-coupled seven-transmembrane proteins that bind a retinaldehyde chromophore in photoreception. Here, we sought potential as yet undescribed avian retinal photoreceptors, focusing on Opsin 3 homologs in the chicken. We found two Opsin 3-related genes in the chicken genome: one corresponding to encephalopsin/panopsin (Opn3) in mammals, and the other belonging to the teleost multiple tissue opsin (TMT) 2 group. Bioluminescence imaging and G protein activation assays demonstrated that the chicken TMT opsin (cTMT) functions as a blue light sensor when forced-expressed in mammalian cultured cells. We did not detect evidence of light sensitivity for the chicken Opn3 (cOpn3). In situ hybridization demonstrated expression of cTMT in subsets of differentiating cells in the inner retina and, as development progressed, predominant localization to retinal horizontal cells (HCs). Immunohistochemistry (IHC) revealed cTMT in HCs as well as in small numbers of cells in the ganglion and inner nuclear layers of the post-hatch chicken retina. In contrast, cOpn3-IR cells were found in distinct subsets of cells in the inner nuclear layer. cTMT-IR cells were also found in subsets of cells in the hypothalamus. Finally, we found differential distribution of cOpn3 and cTMT proteins in specific cells of the cerebellum. The present results suggest that a novel TMT-type opsin 3 may function as a photoreceptor in the chicken retina and brain.

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“…For instance, opsins have been identified beyond the brain and may have other benefits. For instance, early evidence suggests that opsins may have a potential role in treatment of liver cancer [12].…”

mentioning

confidence: 99%

Let There Be Light: Brain\'s Photosensitivity Beyond the Visual System and Its Relevance to Photic Stimulation for Mood and Sleep Disorders

Youssef¹

2013

J Sleep Disorders Ther

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Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating the apoptotic pathway

Cited by 38 publications

References 31 publications

Expression of OPN3 in lung adenocarcinoma promotes epithelial‐mesenchymal transition and tumor metastasis

Expression of OPN3 in lung adenocarcinoma promotes epithelial‐mesenchymal transition and tumor metastasis

Two Opsin 3-Related Proteins in the Chicken Retina and Brain: A TMT-Type Opsin 3 Is a Blue-Light Sensor in Retinal Horizontal Cells, Hypothalamus, and Cerebellum

Let There Be Light: Brain\'s Photosensitivity Beyond the Visual System and Its Relevance to Photic Stimulation for Mood and Sleep Disorders

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