Opportunities for Innovation in Genetic Transformation of Forest Trees

Nagle, Michael; Déjardin, Annabelle; Pilate, Gilles; Strauss, Steven H.

doi:10.3389/fpls.2018.01443

Cited by 39 publications

(27 citation statements)

References 93 publications

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“…Therefore, tissue culture-free methods are highly desirable and necessary for transgenefree genome editing. In planta transformation takes advantage of natural biological processes, which makes it a valuable alternative to in vitro tissue culture and regeneration 132,133 . Various plant cells or tissues can be the ideal transformation targets such as germline or meristematic cells 116,134,135 and dormant buds 136 .…”

Section: Transgene-free Genome Editingmentioning

confidence: 99%

Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement

Sapkota

Knaap

2020

Hortic Res

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Directed breeding of horticultural crops is essential for increasing yield, nutritional content, and consumer-valued characteristics such as shape and color of the produce. However, limited genetic diversity restricts the amount of crop improvement that can be achieved through conventional breeding approaches. Natural genetic changes in cisregulatory regions of genes play important roles in shaping phenotypic diversity by altering their expression. Utilization of CRISPR/Cas editing in crop species can accelerate crop improvement through the introduction of genetic variation in a targeted manner. The advent of CRISPR/Cas-mediated cis-regulatory region engineering (cis-engineering) provides a more refined method for modulating gene expression and creating phenotypic diversity to benefit crop improvement. Here, we focus on the current applications of CRISPR/Cas-mediated cis-engineering in horticultural crops. We describe strategies and limitations for its use in crop improvement, including de novo cis-regulatory element (CRE) discovery, precise genome editing, and transgene-free genome editing. In addition, we discuss the challenges and prospects regarding current technologies and achievements. CRISPR/Cas-mediated cis-engineering is a critical tool for generating horticultural crops that are better able to adapt to climate change and providing food for an increasing world population.

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Section: Transgene-free Genome Editingmentioning

confidence: 99%

Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement

Sapkota

Knaap

2020

Hortic Res

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“…Even though there has been considerable progress enabling in vitro organogenesis and somatic embryogenesis in a variety of crops, transformation is often restricted to very few crop varieties, limiting the application of genome editing and transformation to crop improvement ( Altpeter et al., 2016 ). Expression of developmental genes to boost regeneration has been reported as promising strategy to overcome this hurdle in transformation of plants of agricultural interest ( Nagle et al., 2018 ; Gordon-Kamm et al., 2019 ). Here, we have discovered that the overexpression of the transcription factor GRF5 from Arabidopsis and/or its homologs increases transformation efficiency in sugar beet and maize.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species

et al. 2020

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Successful regeneration of genetically modified plants from cell culture is highly dependent on the species, genotype, and tissue-type being targeted for transformation. Studies in some plant species have shown that when expression is altered, some genes regulating developmental processes are capable of triggering plant regeneration in a variety of plant cells and tissue-types previously identified as being recalcitrant to regeneration. In the present research, we report that developmental genes encoding GROWTH-REGULATING FACTORS positively enhance regeneration and transformation in both monocot and dicot species. In sugar beet ( Beta vulgaris ssp. vulgaris ), ectopic expression of Arabidopsis GRF5 ( AtGRF5 ) in callus cells accelerates shoot formation and dramatically increases transformation efficiency. More importantly, overexpression of AtGRF5 enables the production of stable transformants in recalcitrant sugar beet varieties. The introduction of AtGRF5 and GRF5 orthologs into canola ( Brassica napus L.), soybean ( Glycine max L.), and sunflower ( Helianthus annuus L.) results in significant increases in genetic transformation of the explant tissue. A positive effect on proliferation of transgenic callus cells in canola was observed upon overexpression of GRF5 genes and AtGRF6 and AtGRF9 . In soybean and sunflower, the overexpression of GRF5 genes seems to increase the proliferation of transformed cells, promoting transgenic shoot formation. In addition, the transformation of two putative AtGRF5 orthologs in maize ( Zea mays L.) significantly boosts transformation efficiency and resulted in fully fertile transgenic plants. Overall, the results suggest that overexpression of GRF genes render cells and tissues more competent to regeneration across a wide variety of crop species and regeneration processes. This sets GRFs apart from other developmental regulators and, therefore, they can potentially be applied to improve transformation of monocot and dicot plant species.

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“…Even though there has been considerable progress enabling in vitro organogenesis and somatic embryogenesis in a variety of crops, transformation is often restricted to very few crop varieties, limiting the application of genome editing and transformation to crop improvement (Altpeter et al, 2016). Expression of developmental genes to boost regeneration has been reported as promising strategy to overcome this hurdle in transformation of plants of agricultural interest (Nagle et al, 2018; Gordon-Kamm et al, 2019). Here, we have discovered that the overexpression of the transcription factor GRF5 from Arabidopsis and/or its homologs increases transformation efficiency in sugar beet and maize.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the transcription factor GROWTH-REGULATING FACTOR5 improves transformation of dicot and monocot species

Kong

Martín-Ortigosa

Finer

et al. 2020

Preprint

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1AbstractSuccessful regeneration of genetically modified plants from cell culture is highly dependent on the species, genotype, and tissue-type being targeted for transformation. Studies in some plant species have shown that when expression is altered, some genes regulating developmental processes are capable of triggering plant regeneration in a variety of plant cells and tissue-types previously identified as being recalcitrant to regeneration. In the present research, we report that developmental genes encoding GROWTH-REGULATING FACTORS positively enhance regeneration and transformation in both monocot and dicot species. In sugar beet (Beta vulgaris ssp. vulgaris), ectopic expression of Arabidopsis GRF5 (AtGRF5) in callus cells accelerates shoot formation and dramatically increases transformation efficiency. More importantly, overexpression of AtGRF5 enables the production of stable transformants in recalcitrant sugar beet varieties. The introduction of AtGRF5 and GRF5 orthologs into canola (Brassica napus L.), soybean (Glycine max L.), and sunflower (Helianthus annuus L.) results in significant increases in genetic transformation of the explant tissue. A positive effect on proliferation of transgenic callus cells in canola was observed upon overexpression of GRF5 genes and AtGRF6 and AtGRF9. In soybean and sunflower, the overexpression of GRF5 genes seems to increase the proliferation of transformed cells, promoting transgenic shoot formation. In addition, the transformation of two putative AtGRF5 orthologs in maize (Zea mays L.) significantly boosts transformation efficiency and resulted in fully fertile transgenic plants. Overall, the results suggest that overexpression of GRF genes render cells and tissues more competent to regeneration across a wide variety of crop species and regeneration processes. This sets GRFs apart from other developmental regulators and, therefore, they can potentially be applied to improve transformation of monocot and dicot plant species.

show abstract

Opportunities for Innovation in Genetic Transformation of Forest Trees

Cited by 39 publications

References 93 publications

Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement

Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement

Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species

Overexpression of the transcription factor GROWTH-REGULATING FACTOR5 improves transformation of dicot and monocot species

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