Ophiopogonin D and EETs ameliorate Ang II-induced inflammatory responses via activating PPARα in HUVECs

Huang, Xiaoyan; Wang, Yuguang; Zhang, Zhaoyan; Wei, Yuan; Chen, Xiangmei; Wang, Yi; Gao, Yue

doi:10.1016/j.bbrc.2017.06.007

Cited by 31 publications

(20 citation statements)

References 27 publications

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“…A previous study has indicated that the possible anti-inflammatory mechanism of OP-D is related to the suppression of the NF-kB signaling pathway (16). It was also reported that OP-D suppresses Ang II-induced activation of pro-inflammatory cytokines (IL-6, VCAM-1, and TNF-a) and NF-kB nuclear translocation (17). These activities of OP-D indicate its potential prevention effects in DN.…”

Section: Introductionmentioning

confidence: 81%

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Qiao

Wang

et al. 2020

Braz J Med Biol Res

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Ophiopogonin D (OP-D) is the principal pharmacologically active ingredient from Ophiopogon japonicas, which has been demonstrated to have numerous pharmacological activities. However, its protective effect against renal damage in streptozotocin (STZ)-induced diabetic nephropathy (DN) rats remains unclear. The present study was performed to investigate the protective effect of OP-D in the STZ-induced DN rat model. DN rats showed renal dysfunction, as evidenced by decreased serum albumin and creatinine clearance, along with increases in serum creatinine, blood urea nitrogen, TGF-b1, and kidney hypertrophy, and these were reversed by OP-D. In addition, STZ induced oxidative damage and inflammatory response in diabetic kidney tissue. These abnormalities were reversed by OP-D treatment. The findings obtained in the present study indicated that OP-D might possess the potential to be a therapeutic agent against DN via inhibiting renal inflammation and oxidative stress.

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Section: Introductionmentioning

confidence: 81%

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Qiao

Wang

et al. 2020

Braz J Med Biol Res

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“…We demonstrated that OP-D suppressed drug-induced endoplasmic reticulum stress by upregulating the levels of CYP2J3/EET in cardiomyocytes [18]. Additionally, we found that OP-D exerts endotheliumprotective effects via the CYP2J2/EET/PPARα signaling pathway in HUVECs [19]. Based on our previous studies, we propose that OP-D-induced and SM-I-induced CYP2J3 overexpression and production of circulating 11, 12-EET contribute to protection from MI/R injury.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 86%

“…Our previous studies demonstrated that OP-D and exogenous 11, 12-EET play protective roles as anti-inflammatory and anti-apoptotic agents following Ang IIinduced endothelial injury via activating CYP2J2 and increasing 11, 12-EET production in human umbilical vein endothelial cells (HUVECs) [19,25]. Based on these findings, we tested the hypothesis that OP-D and SM-I exert a protective role in MI/R injury and are associated with increased cardiac expression of CYP2J3 and enhanced circulating 11, 12-EET levels in rats.…”

Section: Op-d Activated Cyp2j3 Expression and Increased Serum And Tismentioning

confidence: 99%

Ophiopogonin D Reduces Myocardial Ischemia-Reperfusion Injury via Upregulating CYP2J3/EETs in Rats

Huang

Wang²,

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Epoxyeicosatrienoic acids (EETs) are cytochrome P450 epoxygenase (CYP) metabolites of arachidonic acid and have multiple cardiovascular effects. Ophiopogonin D (OP-D) is an important effective monomeric component in Shenmai injection (SM-I). Both have been reported to have a variety of biological functions, including anti-inflammatory, anti-oxidant, and anti-apoptotic effects. We previously demonstrated that OP-D–mediated cardioprotection involves activation of CYP2J2/3 and enhancement of circulating EETs levels in vitro and can be developed as a novel drug for the therapy of myocardial ischemia-reperfusion (MI/R) injury. We therefore hypothesized that the protective effects of OP-D and SM-I against MI/R injury are associated with increased expression of CYP2J3 and enhanced circulating 11,12-EET levels in vivo. Methods: A rat model of MI/R injury was generated by ligation of the left anterior descending coronary artery for 40 min, followed by reperfusion for 2 h to determine the protective effects and potential mechanisms of OP-D and SM-I. Electrocardiogram and ultrasonic cardiogram were used to evaluate cardiac function; 2,3,5-triphenyltetrazolium chloride was used to measure myocardial infarct size; hematoxylin and eosin staining and transmission electron microscopy were used to observe the morphology of myocardial tissue; and the expression of related proteins in the mechanistic study was observed by western blot analysis. Results: We found that OP-D and SM-I exert protective effects on MI/R injury, including regulation of cardiac function, reduction of lactate dehydrogenase and creatine kinase production, attenuation of myocardial infarct size, and improvement of the recovery of damaged myocardial structures. We found that OP-D and SM-I activate CYP2J3 expression and increase levels of circulating 11,12-EET in MI/R-injured rats. Conclusion: We tested the hypothesis that the cardioprotective effects of OP-D and SM-I on MI/R injury are associated with increased expression of CYP2J3 and enhanced circulating 11,12-EET levels in rats. Taken together, our results show that the effects of OP-D and SM-I were also mediated by the activation of the PI3K/Akt/eNOS signaling pathway, while inhibition of the NF-κB signaling pathway and antioxidant and anti-apoptotic effects were involved in the cardioprotective effects of OP-D and SM-I.

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“…The study found that OP-D and SM-I act by inducing cardioprotection on MI/R injury by regulating cardiac function, reducing acetate dehydrogenase and creatine kinase (CK) generation, decreasing infarct size, and improving the injured cardiac structures. Cardioprotection by OP-D and SM-I was mediated by activating the phosphoinositide 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) signaling pathway and suppressing the nuclear factor- κ B (NF- κ B) signaling pathway [49, 50].…”

Section: Protective Effects Of Traditional Chinese Medicine (Tcm) mentioning

confidence: 99%

The Role of Traditional Chinese Medicine in the Regulation of Oxidative Stress in Treating Coronary Heart Disease

Yang

Han

et al. 2019

Oxidative Medicine and Cellular Longevity

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Oxidative stress has been closely related with coronary artery disease. In coronary heart disease (CHD), an excess of reactive oxygen species (ROS) production generates endothelial cell and smooth muscle functional disorders, leading to a disequilibrium between the antioxidant capacity and prooxidants. ROS also leads to inflammatory signal activation and mitochondria-mediated apoptosis, which can promote and increase the occurrence and development of CHD. There are several kinds of antioxidative and small molecular systems of antioxidants, such as β-carotene, ascorbic acid, α-tocopherol, and reduced glutathione (GSH). Studies have shown that antioxidant treatment was effective and decreased the risk of CHD, but the effect of the treatment varies greatly. Traditional Chinese medicine (TCM) has been utilized for thousands of years in China and is becoming increasingly popular all over the world, especially for the treatments of cardiovascular diseases. This review will concentrate on the evidence of the action mechanism of TCM in preventing CHD by modulating oxidative stress-related signaling pathways.

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Ophiopogonin D and EETs ameliorate Ang II-induced inflammatory responses via activating PPARα in HUVECs

Cited by 31 publications

References 27 publications

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats

Ophiopogonin D Reduces Myocardial Ischemia-Reperfusion Injury via Upregulating CYP2J3/EETs in Rats

The Role of Traditional Chinese Medicine in the Regulation of Oxidative Stress in Treating Coronary Heart Disease

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