Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3K/Akt signaling pathway

Chen, Meijuan; Du, Yuhong; Qui, Min; Wang, Mingyan; Chen, Kejun; Huang, Zhenzhou; Jiang, Miao; Xiong, Fei; Chen, Jianping; Zhou, Jing; Fengrong, Jiang; Yin, Lian; Tang, Yuping; Ye, Lihong; Zhan, Zhenlin; Duan, Jin‐Ao; Fu, Haian; Xu, Zhonghua

doi:10.3892/or.2012.2131

Cited by 71 publications

(49 citation statements)

References 21 publications

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“…Radix Ophiopogon japonicus is an important medicinal herb widely used for the treatment of various tumors, inflammatory diseases, hepatitis and diarrhea in East Asian countries, including China, Korea, Taiwan, and Japan (18)(19)(20)(21). The plant has been reported to contain a large number of steroidal saponins.…”

Section: Introductionmentioning

confidence: 99%

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

Zhang

Han

Zhai

et al. 2012

Molecular Medicine Reports

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Ophiopogonin-D is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess anti-inflammatory and anti-oxidant properties. The present study was the first to examine the anti-tumor metastasis properties of ophiopogonin-D. An MTT assay showed that ophiopogonin-D inhibited the proliferation of MDA-MB-435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDA-MB-435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogonin-D. Gelatin zymography and western blot analysis showed that ophiopogonin-D inhibited the expression and secretion of matrix metalloproteinase-9 (MMP-9), but not that of MMP-2. Inhibition of phosphorylation of p38 by ophiopogonin-D indicated its inhibition of the mitogen-activated protein kinase pathway. Overall, the results suggested that ophiopogonin-D may be considered as a candidate drug for treating or preventing tumor metastasis.

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Section: Introductionmentioning

confidence: 99%

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

Zhang

Han

Zhai

et al. 2012

Molecular Medicine Reports

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“…Additionally, it has been reported that the overexpression or restoration of miR-135a represses proliferation and facilitates apoptosis of tumor cells by its target genes c-Myc in renal cell carcinoma [16], JAK2 in lymphoma [17], and ROCK1 in gastric cancer [18]. We find that the PI3K/Akt signaling pathway is commonly investigated for its promotion of NSCLC progression in vivo and in vitro as the underlying mechanism of miR-135a in NSCLC [19, 20]. Interestingly, the findings of Ma et al and Salani et al provoked us to speculate that the IGF-1/PI3K/Akt signaling pathway may play an important role in NSCLC [21, 22].…”

Section: Introductionmentioning

confidence: 99%

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer

Zhou

et al. 2017

Cell Physiol Biochem

112

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Objective: This study explored the ability of microRNA-135a (miR-135a) to influence cell proliferation, migration, invasion, apoptosis and tumor angiogenesis through the IGF-1/PI3K/Akt signaling pathway in non-small cell lung cancer (NSCLC). Methods: NSCLC tissues and adjacent normal tissues were collected from 138 NSCLC patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-135a and IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 mRNA; western blotting was used to determine the expression levels of IGF-1, PI3K and Akt protein; and enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression levels of VEGF, bFGF and IL-8 protein. Human NSCLC cell lines (A549, H460, and H1299) and the human bronchial epithelial cell line (HBE) were selected. A549 cells were assigned to blank, negative control (NC), miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. The following were performed: an MTT assay to assess cell proliferation, a scratch test to detect cell migration, a Transwell assay to measure cell invasion, and a flow cytometry to analyze cell apoptosis. Results: The expression level of miR-135a was lower while those of IGF-1, PI3K and Akt mRNA were higher in NSCLC tissues than in the adjacent normal tissues. Dual-luciferase reporter assay indicated IGF-1 as a target of miR-135a. The in vitro results showed that compared with the blank group, cell proliferation, migration and invasion were suppressed, mRNA and protein levels of IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 were reduced, and cell apoptosis was enhanced in the miR-135a mimics and IGF-1 siRNA groups. Compared with the IGF-1 siRNA group, cells in the miR-135a inhibitors + IGF-1 siRNA group demonstrated increased cell proliferation, migration and invasion, elevated mRNA and protein levels of IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 and reduced cell apoptosis. Conclusion: These findings indicated that miR-135a promotes cell apoptosis and inhibits cell proliferation, migration, invasion and tumor angiogenesis by targeting IGF-1 gene through the IGF-1/PI3K/Akt signaling pathway in NSCLC.

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“…In human NSCLC cell lines NCI-H157 and NCI-H460, OP-B was proved to induce autophage of cancer cells via inhibition of the PI3K/Akt signaling pathway (90).…”

Section: Saponinmentioning

confidence: 99%

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Xia

Chen

Zhang

et al. 2014

DD^|^T

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Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3K/Akt signaling pathway

Cited by 71 publications

References 21 publications

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

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