Operational calixarene-based fluorescent sensing systems for choline and acetylcholine and their application to enzymatic reactions

Advanced materials for optical sensing and biosensing of neurotransmitters

“…The selectivity of the host-guest complex was increased by using enzymatic reactions of Chox and AChE at physiological pH. An advantage of the reported method is that it is possible to determine choline and ACh, both separately and together [93].…”

Synthetic trimethyllysine receptors that bind histone 3, trimethyllysine 27 (H3K27me3) and disrupt its interaction with the epigenetic reader protein CBX7

“…The broad selectivity of the calixarenes will in fact facilitate their use as biochemical reagents for the study of multiple pathways, and the promise of using supramolecular reagents for the construction of novel biochemical assays has already been established. 36,37,40 There is also hope for improving their specificity further to the point of being useful for studies into the chemical biology of modification pathways. The structure-function relations identified here have provided us with a basic understanding of the molecular recognition determinants for these synthetic receptors.…”

“…Previous studies have shown that the dye lucigenin is a promiscuous binder of many different sulfonated calixarenes, 39,40 and we found it to be generally well suited to use in a dye-displacement format with each of these compounds. We sought to create a rapid, quantitative assay for H3K27me3 (and H3K27) binding according to the schematic in Fig.…”

Indikatorverdrängungsassays in lebenden Zellen

“…In diesen Fällen sollte die Inkubation der Zellen mit Kompetitoren wie Cholin und Acetylcholin (50 mm) [20] sowie Protamin (200 mm) [14] zu einer sofortigen Abgabe von LCG in das umgebende Medium führen, weil der eigentliche Verdrängungsprozess innerhalb von Millisekunden abläuft. [14] Allerdings zeigten die Analytlçsungen, die nach 10-20 min [18a -d] In der Tat wurden die Analyten nicht nur mit der Maß-gabe einer hohen Affinität ausgewählt, sondern auch einer effizienten Aufnahme in die Zellen.…”