Indikatorverdrängungsassays in lebenden Zellen

Norouzy, Amir; Azizi, Zahra; Nau, Werner M.

doi:10.1002/ange.201407808

Cited by 17 publications

(5 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liberated labeled peptides from their complex enhanced the fluorescent emission of the cocktail. The experiment is the reminiscence of indicator displacement assay (IDA) we have previously reported 29 . Because many hundred times of 1 were needed to displace with 2 or 3 within the complex, we concluded that the labeled peptide bound more strongly to the HLA streptamer.…”

Section: Resultsmentioning

confidence: 84%

“…The experiment is the reminiscence of indicator displacement assay (IDA) we have previously reported. 29 Because many hundred times of 1 were needed to displace with 2 or 3 within the complex, we concluded that the labeled peptide bound more strongly to the HLA streptamer. Besides, the competition binding is another piece of evidence that labeled peptides and the unlabeled peptide have the same binding site on the HLA streptamer.…”

Section: Resultsmentioning

confidence: 87%

See 1 more Smart Citation

Fluorescence labeling of anchor‐modified Mart‐1 peptide for increasing its affinity for HLA‐A*0201: Hit two targets with one arrow

Fattahi

Salehi

Azizi

et al. 2023

Journal of Peptide Science

Self Cite

View full text Add to dashboard Cite

One of the most successful strategies in designing peptide‐based cancer vaccines is modifying natural epitope peptides to increase their binding strength to human leukocyte antigens (HLAs). Anchor‐modified Mart‐1 peptide (ELAGIGILTV) is among the artificial epitope peptides with the highest binding affinity for HLA‐A*0201. In this study, by fluorescence labeling of its either C‐ or N‐terminus with Nε‐(5‐carboxyfluorescein)‐l‐lysine, we not only made it traceable but also drastically increased its binding strength to HLA‐A*0201. HLA streptamer, for the first time, is introduced for measuring the binding constants (Ka) of the labeled peptides. The affinity of the labeled peptides for the HLA‐A*201 of the MCF‐7 cells was extraordinarily high and co‐incubating them with the highest possible amount of the unlabeled peptide, as a competitor, did not significantly prohibit them from binding to the HLA. The reproducibility of the obtained results was confirmed by using the T2 cell line. The HLA‐deficient K562 cell line was used as the negative control. With in silico simulations, we found two hydrophobic pockets on both sides of HLA‐A*0201 for anchoring the C‐ or N‐terminal 5‐carboxyfluorescein probe, which can explain the extraordinary affinity of the labeled peptides for the HLA‐A*0201.

show abstract

Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 87%

Fluorescence labeling of anchor‐modified Mart‐1 peptide for increasing its affinity for HLA‐A*0201: Hit two targets with one arrow

Fattahi

Salehi

Azizi

et al. 2023

Journal of Peptide Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another research direction is the approach of Artificial yntelligence techniques for communication in clusters of multi-agent systems, such in [3], [15].…”

Section: Related Workmentioning

confidence: 99%

Context-aware Control Platform for Sensor Network Integration in IoT and Cloud

Merezeanu¹,

Vasilescu²,

Dobrescu³

2016

SIC

View full text Add to dashboard Cite

“…Through the highly selective qualitative and quantitative detection of a target analyte, its pharmacological activity, chemical toxicity, and bioavailability can be accurately evaluated for various purposes, including medical diagnosis, environmental monitoring, food safety, and drug discovery . As a common design practice, a chemosensor generally comprises a receptor and a signaling unit ensemble that operates by either a displacement or an intrinsic sensing mechanism. Efforts to optimize chemosensing selectivity have been extensive and generally focused on modifying the design of the receptors to differentiate among species such as cations, anions, zwitterions, and functional molecules .…”

Section: Introductionmentioning

confidence: 99%

“…In this study, a catalysis‐based indicator‐displacement assay was developed to selectively determine the analyte, methyl parathion, even under complicated sensing conditions. In recent years, indicator displacement assays (IDA) have attracted considerable attention as they provide good chemosensing selectivity upon interaction with analytes in complex mixtures . The basic principle of IDA is to provide a selective signal resulting from specific analyte competition of the receptor from the indicator–receptor ensemble.…”

Section: Introductionmentioning

confidence: 99%

Catalytic Chemosensing Assay for Selective Detection of Methyl Parathion Organophosphate Pesticide

Zheng

Shen²,

Tang

et al. 2019

Chemistry A European J

View full text Add to dashboard Cite

Herein, a catalytic chemosensing assay (CCA), based on a bimetallic complex, [RuII(bpy)2(CN)2]2(CuII)2 (bpy=2,2′‐bipyridine), is described. This complex integrates a task‐specific catalyst (CuI‐catalyst) and a signaling unit ([RuII(bpy)2(CN)2]) to specifically hydrolyze methyl parathion, a highly toxic organophosphate (OP) pesticide. The bimetallic complex catalyzed the hydrolysis of the phosphate ester to generate o,o‐dimethyl thiophosphate (DTP) anion and 4‐nitrophenolate. Intrinsically, 4‐nitrophenolate absorbed UV/Vis light at λmax=400 nm, creating the first level of the chemosensing signal. DTP interacted with the original complex to displace the chromophore, [RuII(bpy)2(CN)2], which was monitored by spectrofluorometry; this was classified as the second level of chemosensing signal. By integrating both spectroscopic and spectrofluorometric signals with a simple AND logic gate, only methyl parathion was able to provide a positive response. Other aromatic and aliphatic OP pesticides (diazinon, fenthion, meviphos, terbufos, and phosalone) and 4‐nitrophenyl acetate provided negative responses. Furthermore, owing to the metal‐catalyzed hydrolysis of methyl parathion, the CCA system led to the detoxification of the pesticide. The CCA system also demonstrated its catalytic chemosensing properties in the detection of methyl parathion in real samples, including tap water, river water, and underground water.

show abstract

Indikatorverdrängungsassays in lebenden Zellen

Cited by 17 publications

References 76 publications

Fluorescence labeling of anchor‐modified Mart‐1 peptide for increasing its affinity for HLA‐A*0201: Hit two targets with one arrow

Fluorescence labeling of anchor‐modified Mart‐1 peptide for increasing its affinity for HLA‐A*0201: Hit two targets with one arrow

Context-aware Control Platform for Sensor Network Integration in IoT and Cloud

Catalytic Chemosensing Assay for Selective Detection of Methyl Parathion Organophosphate Pesticide

Contact Info

Product

Resources

About