Operation of a 27‐m³ biopile for the treatment of petroleum‐contaminated soil

Abstract

Glucose‐6‐phosphate dehydrogenase (G6PD) deficiency is the most common human enzymopathy, affecting more than 200 million people worldwide. To date more than 123 mutations in the G6PD gene have been discovered, among which 12 point mutations are found in the Chinese. Setting up a simple and accurate method for detecting these mutations is not only useful for diagnosing G6PD deficiency under some circumstances that it is difficult to measure the activity of the enzyme, but also for studying the frequency of the G6PD genotypes. The purpose of this study was to develop a simple, inexpensive and accurate method for detecting these common mutations. Microarray‐based assay was described in this study. Samples from 198 G6PD‐deficient persons were investigated. The DNA sequencing data supported the results obtained by microarray‐based assay. Thus, we concluded that the microarray‐based assay is a rapid, simple, inexpensive, and accurate method for detecting the most common G6PD gene mutations among the Chinese. This method involves the selective amplification of human G6PD gene with specific oligonucleotide primers, fragmentation and labeling of PCR products, followed by hybridization with allele‐specific oligonucleotide (ASO) probes on chip. Am. J. Hematol. 76:405–412, 2004. © 2004 Wiley‐Liss, Inc.

Experimental Investigation of a Pilot-Scale Concerning Ex-Situ Bioremediation of Petroleum Hydrocarbons Contaminated Soils

“…These results were partially validated by submitting a patent application 44 to the Official Bulletin of Industrial Property (OSIM). It may also be mentioned that the obtained yields are comparable with yields obtained in other research conducted with similar treatment conditions: 80-85.2% 23 and 66-75% 24 .…”

“…In order to evaluate the use of the biopile method in bioremediation of soils polluted with oil, Iturbe et colab. conducted several studies: 1) in a former station of storage and distribution of oil, after 66 days of treatment they achieved a remediation efficiency of 85.2% 22 ; 2) at an oil plant in northern Mexico, after 22 weeks of treatment they achieved a remediation efficiency of 80% 23 .…”

Biological Remediation of Petroleum Contaminants

“…In a successful biopile remediation test, the soil microbial characterization showed one Bacillus, two Corynebacterium, and three Mycrococcus species among the existing Gram-positive bacteria, and Enterobacter amnigenus, Enterobacter cloacae, and Pseudomonas sp. were detected as the main Gramnegative bacteria present in the soils (Iturbe et al 2007).…”

Ex-Situ Remediation Technologies for Environmental Pollutants: A Critical Perspective

“…Wu and Crapper ( 2009 ) designed a hydraulic-based approach to stimulate a biopile in the context of its ambient temperature. When a 27 m 3 biopile was operated for 22 weeks, it was able to remove 80 % TPH at full-scale (Iturbe et al 2007 ). A 28 m 3 pneumatically-aerated bioreactor set up in static biopile amended with manure oil compost (40 %) and garden waste compost (20 %) successfully removed 68.7 % petroleum hydrocarbons within 3-4 months treatment time (Kriipsalu and Nammari 2010 ).…”