Opening of liver mitochondrial permeability transition pore in streptozotocin-induced diabetic rats and its inhibition by methanol fraction of Ficus mucoso (Welw) root bark

Oyebode, Olubukola T.; Ogunbiyi, Foluso Olayemi; Olorunsogo, Olufunso O.

doi:10.1016/j.joim.2019.10.001

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…Normally these pores are closed; prolonged opening of mPTPs results in apoptosis and cell death (2,3). Persistent opening of mPTPs can occur in response to virus infections, or to oxidative or chemical-induced stress (4)(5)(6)(7)(8). Opening of mPTPs increases mitochondrial permeability to molecules with a molecular weight of less than 1.5 kD, as well as MPT, resulting in disruption of mitochondrial respiration, swelling and rupture of the mitochondrial membrane, and cell death (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction

Zhao¹,

Guo²,

Wang³

et al. 2022

Journal of Biological Chemistry

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Section: Introductionmentioning

confidence: 99%

A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction

Zhao¹,

Guo²,

Wang³

et al. 2022

Journal of Biological Chemistry

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“…Mitochondrial ROS has been shown to play a signi cant role in this regard (Rimessi et al, 2016). The mitochondrial permeability Transition (mPT) pore opening has been shown to occur in many disease conditions including in ammation (Crompton, 1999;Crouser et al, 2004;Oyebode et al, 2019). The opening of the mPT pore is activated by a number of factors including excessive ranges of calcium, inorganic phosphate and oxidative stress (Halestrap et al, 2004;Halestrap and Richardson, 2015) which results in matrix swelling, enhancement of ATPase activity, Mitochondrial Outer Membrane Permeabilization (MOMP) (Crompton et al, 1999;Bauer and Murphy, 2020).…”

Section: Introductionmentioning

confidence: 99%

Terpene-rich Fractions of Ficus Mucoso (Welw) Modulate Lipopolysaccharide-induced Inflammatory Mediators and Aberrant Permeability of the Inner Mitochondrial Membrane in Murine Animal Model

Oyebode

Olanlokun

Salami

et al. 2021

Preprint

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Ficus mucoso is traditionally used to treat various infections. This study compared the efficacy of terpene-rich fractions of F. mucoso root bark on LPS-induced inflammation, liver mitochondrial permeability transition (mPT) pore, an index of mitochondrial health, and associated pathological alterations. Terpene-Rich fractions of Dichloromethane (TRDF) and Ethylacetate fractions of F. mucoso (TREF) were obtained according to the method described by Ferguson (1956). A single intraperitoneal injection of 1 mg/kg lipopolysaccharide (LPS) were given to mice to induce systemic inflammation for 72hrs. Evaluation of the effects of the TRDF and TREF on levels of pro-inflammatory mediators (TNF-α, IL-1β, IL-6) and antioxidant indices against LPS-induced hepatic damage were carried out. Mitochondrial swelling was monitored spectrophotometrically as well as mitochondrial ATPase activity and lipid peroxidation. This study reveals that TRDF and TREF possess anti-inflammatory potentials which is related to reduction in levels of pro-inflammatory cytokines, restoration of antioxidant status, reduction in levels of liver marker enzymes as well as associated pathological injury. Furthermore, LPS caused induction of opening of the liver mPT pore which was significantly inhibited by TRDF at 100 and 200 mg/kg bw by 71% and 88% respectively, but only at 100 mg/kg TREF. Furthermore, mitochondrial ATPase activity was inhibited largely by TRDF. The UPLC-ESI-MS analysis revealed the presence of terpenoid derivatives and a few aromatic metabolites in TRDF. The terpene dominance of TRDF metabolites was further justified on the 1H NMR fingerprint.Overall, TRDF is more effective as a cocktail of anti-inflammatory compounds than TREF against LPS-induced acute systemic inflammation.

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Section: Introductionmentioning

confidence: 99%

Terpene-rich fractions of Ficus mucoso (Welw) modulate lipopolysaccharide-induced inflammatory mediators and aberrant permeability of the inner mitochondrial membrane in murine animal model

et al. 2021

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Ficus mucoso is traditionally used to treat various infections. This study compared the e cacy of terpene-rich fractions of F. mucoso root bark on LPS-induced in ammation, liver mitochondrial permeability transition (mPT) pore, an index of mitochondrial health, and associated pathological alterations. Terpene-Rich fractions of Dichloromethane (TRDF) and Ethylacetate fractions of F. mucoso (TREF) were obtained according to the method described by Ferguson (1956). A single intraperitoneal injection of 1 mg/kg lipopolysaccharide (LPS) were given to mice to induce systemic in ammation for 72hrs. Evaluation of the effects of the TRDF and TREF on levels of pro-in ammatory mediators (TNF-α, IL-1β, IL-6) and antioxidant indices against LPS-induced hepatic damage were carried out. Mitochondrial swelling was monitored spectrophotometrically as well as mitochondrial ATPase activity and lipid peroxidation.This study reveals that TRDF and TREF possess anti-in ammatory potentials which is related to reduction in levels of pro-in ammatory cytokines, restoration of antioxidant status, reduction in levels of liver marker enzymes as well as associated pathological injury. Furthermore, LPS caused induction of opening of the liver mPT pore which was signi cantly inhibited by TRDF at 100 and 200 mg/kg bw by 71% and 88% respectively, but only at 100 mg/kg TREF. Furthermore, mitochondrial ATPase activity was inhibited largely by TRDF. The UPLC-ESI-MS analysis revealed the presence of terpenoid derivatives and a few aromatic metabolites in TRDF. The terpene dominance of TRDF metabolites was further justi ed on the 1 H NMR ngerprint.Overall, TRDF is more effective as a cocktail of anti-in ammatory compounds than TREF against LPSinduced acute systemic in ammation.

show abstract

Opening of liver mitochondrial permeability transition pore in streptozotocin-induced diabetic rats and its inhibition by methanol fraction of Ficus mucoso (Welw) root bark

Cited by 7 publications

References 30 publications

A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction

A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction

Terpene-rich Fractions of Ficus Mucoso (Welw) Modulate Lipopolysaccharide-induced Inflammatory Mediators and Aberrant Permeability of the Inner Mitochondrial Membrane in Murine Animal Model

Terpene-rich fractions of Ficus mucoso (Welw) modulate lipopolysaccharide-induced inflammatory mediators and aberrant permeability of the inner mitochondrial membrane in murine animal model

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