Open-type capillary-assembled microchip for rapid, single-step, simultaneous multi-component analysis of serum sample

Microfluidic enzymatic biosensing systems: A review

“…If no sophisticated microfluidic features are (Dungchai et al, 2010) required, glass capillaries are a simple alternative to channel networks produced in polymers or glass (Fig. 6C) (Inadumi et al, 2007;Kimura et al, 2012). One of the most typical structures integrated into microfluidics and used for transduction are electrodes.…”

Advancements in Capillary-Assembled Microchip (CAs-CHIP) Development for Multiple Analyte Sensing and Microchip Electrophoresis

“…39 Most recently, we have successfully analyzed both glucose and cholesterol in serum samples by using the ERC technology. 42 Furthermore, an electrochemical detection system embedded into the CAs-CHIP system has been demonstrated for glucose analysis. 43 …”

“…Instead of an organic analyte-specific fluorescent dye reagent immobilized inside the capillary, an enzyme is immobilized together with PEG on the four corners of the capillary. [41][42] The first demonstrated application of this method was in the analysis of glucose and fluorescein-β-Dgalactopyranoside by using an enzyme-release capillary (ERC). This capillary is stable for at least 15 days when stored at temperatures of about -18 C. 41 The combination of an ionselective optode membrane and an ERC has been explored for urea analysis, according to the sensing mechanism as described in the previous section of this review.…”

“…42 The CAs-CHIP 2G is simply fabricated by embedding the capillary biosensors onto the black PDMS microchannel without the PDMS cover used in the previous CAs-CHIP. Thus, more reproducible chip fabrication and simple fluid handling were achieved (Fig.…”

Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary-Isoelectric Focusing with Rhodamine 110-based Substrates

“…[12][13][14][15][16][17][18][19][20][21][22][23][24][25] Especially, for enzyme activity assays, 18-23 the inner surface of a capillary is covered with a soluble coating containing reagents for the enzyme activity assay. Introducing a sample solution containing enzymes into the RRCs by a capillary action allows for the spontaneous release of the reagents and subsequent mixing with the solution.…”