Microfluidic enzymatic biosensing systems: A review

Mross, Stefan; Pierrat, Sébastien; Zimmermann, Tom; Kraft, Michaël

doi:10.1016/j.bios.2015.03.049

Cited by 71 publications

(54 citation statements)

References 147 publications

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“…An important part of such research activity has focused on the development of enzymatic sensors based on electrochemical principles. [10][11][12] Typically, these sensors use enzymes as electrochemical 3 transduction elements aiming to detect other relevant analytes such as lactate, glucose and urea through enzymatic reactions taking place in the electrode where enzymes are immobilized. 13,14 A remarkable example of that is the glucose sensor based on glucose oxidase, which changed the lives of millions patients suffering from diabetes.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Real-Time Quantification of Enzyme Levels by Interferometric Spectroscopy Combined with Gelatin-Modified Nanoporous Anodic Alumina Photonic Films

et al. 2015

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Herein, we present an interferometric sensor based on the combination of chemically functionalized nanoporous anodic alumina photonic films (NAA-PFs) and reflectometric interference spectroscopy (RIfS) aimed to detect trace levels of enzymes by selective digestion of gelatin. The fabrication and sensing performance of the proposed sensor were characterized in real-time by estimating the changes in effective optical thickness (i.e., sensing principle) of gelatin-modified NAA-PFs (i.e., sensing element) during enzymatic digestion. The working range (WR), sensitivity (S), low limit of detection (LLoD), and linearity (R(2)) of this enzymatic sensor were established by a series of experiments with different concentrations of gelatin (i.e., specific chemical sensing element) and trypsin (i.e., analyte), a model protease enzyme with relevant implications as a biomarker in the diagnosis of several diseases. The chemical selectivity of the sensor was demonstrated by comparison of gelatin digestion by other nonspecific enzyme models such as chymotrypsin and horseradish peroxidase. Furthermore, the role of the chemical sensing element (i.e., gelatin) was assessed by using hemoglobin instead of gelatin. Finally, we demonstrated that this sensor can be readily used to establish the kinetic parameters of enzymatic reactions. The obtained results revealed that the presented sensor has a promising potential to be used as a point-of-care system for fast detection of gastrointestinal diseases at early stages.

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Section: Introductionmentioning

confidence: 99%

Label-Free Real-Time Quantification of Enzyme Levels by Interferometric Spectroscopy Combined with Gelatin-Modified Nanoporous Anodic Alumina Photonic Films

et al. 2015

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“…As mentioned previously, the distinct characteristics of thread make it a useful material for the fabrication microfluidic devices, such as the simplicity, low cost, lightweight, easy access, functionality as well as suitability for mass fabrication methods like sewing or knitting (Li et al, 2009;Mross et al, 2015). It also facilitates the fabrication of 3D structures and can be easily incorporated into garment due to its higher tensile strength than paper (Nilghaz et al, 2013).…”

Section: Fabrication Techniques In Thread-based Microfluidicsmentioning

confidence: 99%

“…Microfluidics is a powerful tool for downscaling devices, especially the flow-based ones, by providing an integrated platform including sample preparation, delivery and measurement with a high degree of automation, only small amounts of reagents and faster assay time (Chen et al, 2016;Mross et al, 2015;Sackmann et al, 2014;Streets and Huang, 2013). Microfluidics is a powerful tool for downscaling devices, especially the flow-based ones, by providing an integrated platform including sample preparation, delivery and measurement with a high degree of automation, only small amounts of reagents and faster assay time (Chen et al, 2016;Mross et al, 2015;Sackmann et al, 2014;Streets and Huang, 2013).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in thread-based microfluidics for diagnostic applications

Weng

Kang

Guo

et al. 2019

Biosensors and Bioelectronics

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Keywords:Thread-based microfluidics Point-of-care Cotton Colorimetric Electrochemical A B S T R A C T Over the past decades, researchers have been seeking attractive substrate materials to keep microfluidics improving to outbalance the drawbacks and issues. Cellulose substrates, including thread, paper and hydrogels are alternatives due to their distinct structural and mechanical properties for a number of applications. Thread have gained considerable attention and become promising powerful tool due to its advantages over paper-based systems thus finds numerous applications in the development of diagnostic systems, smart bandages and tissue engineering. To the best of our knowledge, no comprehensive review articles on the topic of thread-based microfluidics have been published and it is of significance for many scientific communities working on Microfluidics, Biosensors and Lab-on-Chip. This review gives an overview of the advances of thread-based microfluidic diagnostic devices in a variety of applications. It begins with an overall introduction of the fabrication followed by an in-depth review on the detection techniques in such devices and various applications with respect to effort and performance to date. A few perspective directions of thread-based microfluidics in its development are also discussed. Thread-based microfluidics are still at an early development stage and further improvements in terms of fabrication, analytical strategies, and function to become low-cost, low-volume and easy-to-use pointof-care (POC) diagnostic devices that can be adapted or commercialized for real world applications.

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“…Fast and accurate detection of glucose is important in a vast range of application fields such as clinical diagnosis, diabetes management, biological analysis, beverage industry and environmental monitoring, numerous processes and methodologies have been developed for creating new glucose biosensors [1][2][3][4][5][6][7][8][9]. Among them, the amperometry acts as the typical representative of the electrochemical technique and is featured as its unbeaten sensitivity and selectivity by applying a constant bias potential.…”

Section: Introductionmentioning

confidence: 99%