Open pulled straw vitrification of in vitro matured sheep oocytes using different cryoprotectants

Bhat, M. H.; Yaqoob, Syed Hilal; Khan, Firdous Ahmad; Waheed, Shahina; Sharma, Virag; Vajta, Gábor; Ganai, Nazir Ahmad; Shah, Riaz Ahmad

doi:10.1016/j.smallrumres.2012.12.017

Cited by 15 publications

(10 citation statements)

References 24 publications

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“…It has been shown that DMSO facilitates the permeability of EG, which may have a beneficial effect on spindle polymerization and consequently a protective effect during oocyte vitrification [132]. EG is usually combined with DMSO to freeze and vitrify oocytes [39,133] and optimal concentrations have been studied in pigs [134]. However, vitrification of oocytes using the combination of EG and PROH provides better results than those using EG and DMSO in bovine [135] and humans [136].…”

Section: The Type Of Cryoprotectantsmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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Section: The Type Of Cryoprotectantsmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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“…It is therefore important to minimize the damage caused to cells by the osmotic stress or chemical toxicity (Arav, 2014). Survival of sheep in vitro matured oocytes may be improved by step wise dilution of the cryoprotectant, which enabled high survival, cleavage and blastocyst rates of the vitrified oocytes (Bhat et al, 2013). These authors have been conducting research to improve sheep oocyte vitrification.…”

Section: Cryoprotectantsmentioning

confidence: 99%

“…The same was observed for blastocyst production with rates of 18% for EG and 10% for DMSO. The authors suggested that EG would be the CP of choice due its high penetration in oocytes with, in turn, a quick removal after warming (Bhat et al, 2013).…”

Section: Cryoprotectantsmentioning

confidence: 99%

“…The Table 1 compiles some studies on oocyte vitrification in goats and sheep using different CP and later in vitro analysis. (Ebrahimi et al, 2010a;Fernández-Reyez et al, 2012;Rao et al, 2012;Bhat et al, 2013). The objective of these techniques is to reduce the volume of solution and improve the contact with nitrogen to increase the cooling speed and allows the occurrence of vitrification of solution even with lower concentrations of CP (less toxic).…”

Section: Cryoprotectantsmentioning

confidence: 99%

“…In addition, the cell cycle of the oocyte is finely controlled and stopped at very specific stages, requiring taking into account the specific nuclear configuration of these stages. Cryopreservation of oocytes is more difficult than zygotes and later stage embryos (Kharche et al, 2005) and this technique is a challenging task because of its sensitive nature to chilling (Bhat et al, 2013) and to toxic effects of cryoprotectants. However, this biotechnological tool has a number of potential applications such as biodiversity maintenance and development of breeding programs.…”

Section: Introductionmentioning

confidence: 99%

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Factors that affect oocyte vitrification in small ruminants

Chaves

Souza-Fabjan

Mermillod

et al. 2014

RBCV

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At the end of its growth, the mammalian oocyte, include in preovulatory follicle, is the largest cell of the organism, with about 120 µm in diameter. The size of oocyte, together with the specific arrangement of its organels and cytoskeleton, makes a real challenge for cryopreservation techniques. For such large cell, methods that do not require equilibrium to cryopreservation, such as vitrification, are more promising. It is important to highlight that the cryopreservation of oocytes is more difficult than zygotes or later stage embryos and this technique is a challenging task because of oocyte sensitive nature to chilling and toxic effects of cryoprotectants. However, the development of a reliable method for oocyte cryopreservation would be an important advance in the field of reproductive biology for the preservation of genetic resources. The vitrification of mammalian oocytes is influenced by many variables, such as different cryoprotectants, vitrification techniques, presence or absence of cumulus cells, the oocyte structure, metabolism (such as level of lipid storage) and meiotic stage. Thus, all these factors should be considered to optimize the techniques and adapt them to oocytes from each species. Therefore, the present review aims to describe the main factors that affect oocyte vitrification in sheep and goats, reporting the main findings in both species, as well as perspectives of future improvements.Keywords: COC, cryopreservation, gamete, goat, sheep. ResumoNo fim do seu crescimento, o oócito mamífero é a maior célula do organismo, com cerca de 120 µm de diâmetro. O tamanho do oócito em conjunto com a disposição específica das organelas e do citoesqueleto faz com que ele represente um verdadeiro desafio para as técnicas de criopreservação. Para grandes células, métodos que não exigem equilíbrio para criopreservação, como a vitrificação, são mais promissores. É importante ressaltar que a criopreservação de oócitos é mais difícil que de zigotos ou embriões em estádios mais tardios e esta técnica representa um desafio devido à natureza sensível do oócito ao resfriamento e aos efeitos tóxicos de crioprotetores. Entretanto, o desenvolvimento de uma técnica confiável para a criopreservação oocitária representaria um avanço importante para a preservação de recursos genéticos. A vitrificação de oócitos em mamíferos é influenciada por muitas variáveis , tais como diferentes crioprotetores, técnicas de vitrificação, presença ou ausência de células do cumulus, estrutura do oócito, metabolismo (como o nível de armazenamento de lipídios) e estágio meiótico. Assim, todos esses fatores devem ser considerados quando o objetivo é otimizar as técnicas e adaptá-las para oócitos de cada espécie. Assim, a presente revisão tem como objetivo descrever os principais fatores que afetam a vitrificação de oócitos em ovinos e caprinos, relatando os principais resultados em ambas as espécies, bem como as perspectivas de melhorias futuras.

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Meiotic maturation and developmental capability of ovine oocytes at germinal vesicle stage following vitrification using different cryodevices

Quan

Wang

et al. 2016

Cryobiology

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Open pulled straw vitrification of in vitro matured sheep oocytes using different cryoprotectants

Cited by 15 publications

References 24 publications

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Factors that affect oocyte vitrification in small ruminants

Meiotic maturation and developmental capability of ovine oocytes at germinal vesicle stage following vitrification using different cryodevices

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