OP-1 Compared with Iliac Crest Autograft in Instrumented Posterolateral Fusion

Delawi, Diyar; Jacobs, Wilco; Susante, Job L C van; Rillardon, L.; Prestamburgo, Domenico; Specchia, Nicola; Verschoor, Nico; García-Fernández, Carlos; Guerado, Enrique; Ufford, Henriëtte Quarles van; Kruyt, Moyo C.; Dhert, Wouter J.A.; Öner, F. Cumhur

doi:10.2106/jbjs.o.00209

Cited by 36 publications

(19 citation statements)

References 31 publications

(48 reference statements)

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“…This agrees with the current finding that the co-delivery of these factors creates a potent stimulus for new bone formation. The current clinical practice of delivering single supra-physiological doses of BMP-2 could be the reason for its unpredictable induction of new bone formation (Delawi et al, 2016;Hustedt et al, 2014;Vaccaro et al, 2008). Therefore, it is of great interest to elucidate if this co-delivery approach may indeed result in a reduction of the minimal effective BMP dose, considering the high costs (Garrison et al, 2010) and side effects (Carragee et al, 2011b;Epstein, 2013) associated with its current use.…”

Section: Croes Et Almentioning

confidence: 99%

“…Currently, the bone morphogenetic proteins (BMPs) are the only osteoinductive alternatives for bone grafting in the treatment of large bone defects (Carreira et al, 2014;Ronga et al, 2013). However, their current dosage and delivery method is associated with serious adverse effects (Carragee et al, 2011b;Epstein, 2013), high costs (Garrison et al, 2010) and unpredictable outcomes (Delawi et al, 2016;Hustedt et al, 2014;Vaccaro et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Local induction of inflammation affects bone formation

Croes

Kruyt

Loozen

et al. 2017

eCM

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To explore the influence of inflammatory processes on bone formation, we applied a new in vivo screening model. Confined biological pockets were first created in rabbits as a response to implanted bone cement discs. These biomembrane pockets were subsequently used to study the effects of inflammatory stimuli on ectopic bone formation within biphasic calcium phosphate (BCP) constructs loaded with TNF-α, lipopolysaccharide (LPS) or lipoteichoic acid (LTA), all with or without bone morphogenetic protein (BMP)-2. Analysis of bone formation after 12 weeks demonstrated that the inflammatory mediators were not bone-inductive in combination with the BCP alone, but inhibited or enhanced BMP-induced bone formation. LPS was associated with a strong inhibition of bone formation by BMP-2, while LTA and TNF-α showed a positive interaction with BMP-2. Since the biomembrane pockets did not interfere with bone formation and prevented the leakage of pro-inflammatory compounds to the surrounding tissue, the biomembrane model can be used for in vivo approaches to study local inflammation in conjunction with new bone formation. Using this model, it was shown that the modulation of the inflammatory response could be beneficial or detrimental to the subsequent bone formation process. The co-delivery of inflammatory factors and bone-related growth factors should be further explored as a strategy to enhance the bone-forming efficacy of bone substitutes.

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Section: Croes Et Almentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Local induction of inflammation affects bone formation

Croes

Kruyt

Loozen

et al. 2017

eCM

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“…Delawi et al 163 in a prospective randomized study (level 1) evaluated OP-1 for single level instrumented posterolateral fusion surgery for degenerative or isthmic spondylolisthesis. 119 patients undergoing doi: 10.14444/3033…”

Section: Osteogenic Protein 1 (Op-1) In Lumbar Spinementioning

confidence: 99%

Bone substitutes and expanders in Spine Surgery: A review of their fusion efficacies

et al. 2016

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Study Design A narrative review of literature. ObjectiveThis manuscript intends to provide a review of clinically relevant bone substitutes and bone expanders for spinal surgery in terms of efficacy and associated clinical outcomes, as reported in contemporary spine literature. Summary of Background DataEver since the introduction of allograft as a substitute for autologous bone in spinal surgery, a sea of literature has surfaced, evaluating both established and newly emerging fusion alternatives. An understanding of the available fusion options and an organized evidence-based approach to their use in spine surgery is essential for achieving optimal results. MethodsA Medline search of English language literature published through March 2016 discussing bone graft substitutes and fusion extenders was performed. All clinical studies reporting radiological and/or patient outcomes following the use of bone substitutes were reviewed under the broad categories of Allografts, Demineralized Bone Matrices (DBM), Ceramics, Bone Morphogenic proteins (BMPs), Autologous growth factors (AGFs), Stem cell products and Synthetic Peptides. These were further grouped depending on their application in lumbar and cervical spine surgeries, deformity correction or other miscellaneous procedures viz. trauma, infection or tumors; wherever data was forthcoming. Studies in animal populations and experimental in vitro studies were excluded. Primary endpoints were radiological fusion rates and successful clinical outcomes. ResultsA total of 181 clinical studies were found suitable to be included in the review. More than a third of the published articles (62 studies, 34.25%) focused on BMP. Ceramics (40 studies) and Allografts (39 studies) were the other two highly published groups of bone substitutes. Highest radiographic fusion rates were observed with BMPs, followed by allograft and DBM. There were no significant differences in the reported clinical outcomes across all classes of bone substitutes. ConclusionsThere is a clear publication bias in the literature, mostly favoring BMP. Based on the available data, BMP is however associated with the highest radiographic fusion rate. Allograft is also very well corroborated in the literature. The use of DBM as a bone expander to augment autograft is supported, especially in the lumbar spine. Ceramics are also utilized as bone graft extenders and results are generally supportive, although limited. The use of autologous growth factors is not substantiated at this time. Cell matrix or stem cell-based products and the synthetic peptides have inadequate data. More comparative studies are needed to evaluate the efficacy of bone graft substitutes overall.keywords: bone substitutes, allografts, demineralized bone matrix (dbm), spine fusion, ceramics, β tricalcium phosphate (β-tcp), hydroxyapatite, rhbmp-2, osteogenic protein-1 (op-1), autologous growth factors, i-factor, synthetic peptides volume 10 article 33

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“…The situation is exacerbated by a rising number of devastating traumatic war injuries for which often no other treatment option than amputation exists due to the extensive loss of bone and soft tissue6. Current treatment options are associated with high morbidity789 or deficient efficacy10. Mesenchymal stem cells (MSCs) in combination with various scaffolds are under intensive investigation and show promising achievements11121314.…”

mentioning

confidence: 99%

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Bondarava¹,

Cattaneo²,

Ren³

et al. 2017

Sci Rep

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Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9. Ca2+ depositions were observed after two weeks of osteogenic induction which visibly increased when either type of BMP was added. mRNA levels of alkaline phosphatase (ALP) and osteocalcin (OCN) increased when cultured in OM alone but addition of BMP-2, BMP-7 or BMP-9 caused significantly higher expression levels of ALP and OCN. Immunofluorescent staining for OCN, osteopontin and sclerostin supported the observed real-time-PCR data. BMP-9 was the most effective osteogenic inducer in this system. Our findings reveal that tissue regeneration can be remarkably simplified by omitting prior cell isolation and propagation, therefore removing significant obstacles on the way to clinical applications of much needed regeneration treatments.

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OP-1 Compared with Iliac Crest Autograft in Instrumented Posterolateral Fusion

Abstract: Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.

Cited by 36 publications

References 31 publications

Local induction of inflammation affects bone formation

Local induction of inflammation affects bone formation

Bone substitutes and expanders in Spine Surgery: A review of their fusion efficacies

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

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