Oocyte Storage

Al-Hasani, S.; Diedrich, K.; Ludwig, Michael

doi:10.1007/978-3-642-60961-9_28

Cited by 2 publications

(4 citation statements)

References 28 publications

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“…Al- Hasani and Diedrich (1995), using electron microscopy, demonstrated a reduction in the number of cortical granules in thawed rabbit and human oocytes. Contrasting data have been reported by Gook et al (1993), who found an abundance of cortical granules in the cytoplasm of PROH cryopreserved oocytes.…”

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confidence: 98%

“…Pickering et al (1991) found that exposing human oocytes to DMSO for an inadequate time reduces the fertilization rate. (Al Hasani and Diedrich 1995) suggested that an exposure time of 10 min could be suitable for improving the survival rate of human oocytes.…”

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confidence: 98%

“…A human oocyte that is 130 mm in diameter, requires a longer time to reach osmotic balance with the cryoprotectant solution. On the contrary, a sperm whose size is about 1:180 of the size of oocyte, requires a shorter time to reach the osmotic balance leading to better results after freezing-thawing procedures (Al Hasani and Diedrich 1995).…”

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confidence: 99%

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Cryopreservation of Human Oocytes and Ovarian Tissue

Fabbri

2006

Cell Tissue Banking

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Oocyte cryopreservation has the potential to be an important adjunct to assisted reproductive technologies and bypasses some ethical, moral, and religious dilemmas posed by human embryo cryopreservation. The success of human oocyte cryopreservation depends on morphological and biophysical factors that could influence oocyte survival after thawing. Among the morphological factors, the maturity, quality, size of the oocyte, the presence or the absence of the cumulus oophorus seems to play an important role in oocyte survival after thawing. The main biophysical factor of cellular disruption during cryopreservation process in the intracellular ice formation that can be avoided by an adequate cell dehydration; thus reducing the intracellular water by increasing the dehydration process we can limit the damages of the cryopreservation procedure. The dehydration process can be affected by the presence and concentration of the cryoprotectants in the freezing solutions (equilibration and loading solutions), and by the freezing and thawing rate. Two additional properties of cryoprotectants help to protect cells during slow cooling, when the cells are very dehydrated and are surrounded by concentrated salts. The cryoprotectants appear to reduce damage caused by high levels of salt, a property known as salt buffering. Some events occurring to the oocyte during cryopreservation procedure has been found to be a premature exocitosis of cortical granules, leading to an intempestive zona hardening and consequently to a reduction of fertilization rate, and the cryoinjury to the zona pellucida leading to a polispermic fertilization. ICSI is an efficient method to by pass these two events and to achieve a satisfactory outcome in terms of normal fertilization of cryopreserved oocytes. The application of the ICSI to cryopreserved oocytes did not seem to increase the degeneration rate after insemination with respect to fresh oocytes. The increased oocyte survival rate and the use of ICSI have facilitated the recent increase in the number of pregnancies and live birth.

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Cryopreservation of Human Oocytes and Ovarian Tissue

Fabbri

2006

Cell Tissue Banking

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“…Before lipid analysis, straws were thawed by exposure to the air for 10 s at 25ЊC and immersed immediately in water at 37ЊC for 20 s. The COCs were denuded and washed three times in PBS containing 0.3% BSA. Intact oocytes with no discoloration of the ooplasm, cell disruption or pyknosis or those that had a dark ooplasm were selected according to the criteria described by Al Hasani and Diedrich (1995). Selected frozen-thawed oocytes were washed twice in PBS containing 0.1% BSA and then washed in PBS without BSA.…”

Section: Freezing and Thawingmentioning

confidence: 99%

Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes

Kim¹

2001

Reproduction

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ResearchThe lipid content and fatty acid composition of fresh immature and in vitro matured bovine oocytes cultured in media with or without serum, and also those of frozen-thawed immature oocytes were analysed. All oocytes were ranked (A or B) on the basis of their cytoplasmic quality. Fatty acid composition (mol %; w/w) in the total lipid fraction was analysed by gas chromatography. Triglyceride, total cholesterol, phospholipid (phosphocholine-containing phospholipid) and non-esterified fatty acid contents of immature and in vitro matured oocytes were determined using lipid analysis kits. Phosphocholine-containing phospholipid and non-esterified fatty acid contents were determined in frozen-thawed immature bovine oocytes. Palmitic acid was the most abundant fatty acid in immature oocytes (A: 35%, B: 36%), and in in vitro matured oocytes cultured in the medium containing serum (A: 36%, B: 35%) or polyvinyl alcohol (A: 33%, B: 36%). Oleic acid was the second most abundant fatty acid in all A ranked oocytes, whereas stearic acid was the second most abundant fatty acid in all B ranked oocytes. There were significant differences (P < 0.05) in linoleic and arachidonic acid fractions between A and B ranked immature oocytes. In vitro matured oocytes had significantly (P < 0.05) lower proportions of linoleic and arachidonic acids, and significantly (P < 0.01) lower contents of triglyceride and total cholesterol compared with those of immature oocytes. The fatty acid composition of in vitro matured oocytes cultured in medium containing fetal calf serum or polyvinyl alcohol was similar, but significant differences (P < 0.01) in triglyceride and the total cholesterol content were observed. There was a significant decrease (P < 0.05) in the arachidonic acid proportion in frozen-thawed immature oocytes compared with that in fresh immature oocytes. In addition, significant (P < 0.05) decreases in both phospholipid (15.8-10.6 pmol) and non-esterified fatty acid (11.0-4.1 pmol) were found in frozen-thawed immature oocytes. The results indicate that lipids are available for use as an energy source for maturation and that serum lipids are incorporated into the oocyte cytoplasm during in vitro maturation. The changes in the lipid content (mainly phospholipid) and fatty acid composition were also observed in frozen-thawed immature oocytes. The study indicates that the alteration of fatty acid composition in bovine oocytes might improve maturation and cryopreservation.

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Oocyte Storage

Cited by 2 publications

References 28 publications

Cryopreservation of Human Oocytes and Ovarian Tissue

Cryopreservation of Human Oocytes and Ovarian Tissue

Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes

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