Cryopreservation of Human Oocytes and Ovarian Tissue

Fabbri, Raffaella

doi:10.1007/s10561-005-1969-7

Cited by 52 publications

(40 citation statements)

References 66 publications

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“…However, other studies have reported better human MII oocyte survival if the cumulus was removed prior to freezing (Gook et al 1993) and, since prior cumulus removal allows better evaluation of oocyte quality ( Fabbri 2006) and any detrimental effects of removing the cumulus on subsequent zona penetrability are circumvented by using intracytoplasmic sperm injection rather than conventional IVF to achieve fertilisation, cumulus removal prior to cryopreservation is now a standard practice during cryopreservation of MII human oocytes. Moreover, recent clinical studies have produced very promising results in terms of percentages of injected oocytes developing to the blastocyst stage or resulting in ongoing pregnancies (Cobo et al 2008, Cao et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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Vitrifying oocytes is a potentially valuable means of preserving the female germ line, but significantly compromises oocyte developmental competence. This study examined the hypothesis that the cumulus complex protects the oocyte during vitrification. Vitrified-warmed immature cumulus oocyte complexes (COCs) were labelled with a plasma membrane impermeant DNA marker (ethidium homodimer-1) to examine the percentage and location of dead cumulus cells, and to investigate the effect of the proportion of dead cells (C1,C2 or C3) on the success of in vitro maturation (IVM). Further, oocytes were labelled for connexin-43 or injected with Lucifer yellow dye to determine whether the integrity of the gap junctions between an oocyte and its cumulus was compromised by vitrification. Finally, the effect of denuding immature and mature oocytes on their ability to withstand vitrification was examined. Cryopreserving immature COCs increased the number of dead cumulus cells (13 vs 2.6% for controls; P!0.05). However, an increased proportion of dead cumulus cells did not affect post-warming maturation rates (w30% MII ) presumably because dead cells were located at the periphery of the cumulus mass and cumulus-oocyte gap junction communication was not disrupted. Moreover, cumulus removal prior to IVM or vitrification indicated that while the cumulus does protect immature oocytes during vitrification it does so by mechanisms other than support during maturation. Cumulus presence was also found to protect mature equine oocytes against vitrification-induced damage since cumulus-enclosed MII oocytes preserved their meiotic spindle quality better during vitrification than denuded oocytes (38.1 vs 3.1% normal spindles; P!0.05).

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Section: Discussionmentioning

confidence: 99%

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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“…Logo após a desocngelação, é necessária a remoção do ACP, que pode ser realizada através de uma ou várias lavagens do material criopreservado (Santos et al, 2008); comumente realiza-se três lavagens, com duração entre 5 a 15 minutos cada uma. O procedimento de remoção do crioprotetor é um fator que pode afetar a sobrevivência celular pós descongelação, uma vez que ao expor a célula com alta concentração de ACP a um meio com concentração deste agente baixa ou nula, a água tende a penetrar rapidamente na célula, causando aumento de volume ou até mesmo rompimento celular (Fabbri, 2006).…”

Section: Congelação Lentaunclassified

Advances in ovarian tissue cryopreservation in goats and sheep

2014

AVB

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RESUMO -A criopreservação de tecido ovariano, sem dúvidas, tem se tornado, uma prática não apenas suplementar as técnicas de reprodução assistida, mas também fundamental para a preservação da fertilidade da fêmea. Isso tem sido observado, especialmente na espécie humana, cuja espécie em uma década de trabalho, já se soma aproximadamente trinta nascimentos de bebês saudáveis oriundos de ovário criopreservado. No entanto, todo o avanço que hoje é estabelecido, muito se deve aos trabalhos pioneiros realizados em animais de animais de produção como os ovinos. Relatos de sucesso após criopreservação e transplante de tecido ovariano no início dos anos 90 impulsionaram importantes equipes a investir esforços e dedicação nessa área, voltados para a reprodução humana, e embora, o sucesso seja evidente, os avanços da criopreservação de tecido ovariano tanto na espécie ovina, como na espécie caprina são ainda bastante limitados. Portanto, o objetivo desse trabalho, é apresentar uma retrospectiva dos principais resultados obtidos com a criopreservação de tecido ovariano nessas duas espécies.Palavras-Chave: congelação lenta; vitrificação; função ovariana; folículo pré-antral.ABSTRACT -Cryopreservation of ovarian tissue, no doubt, has been considerated not only as an additional practice to assisted reproductive techniques, but it is fundamental for female fertility preservation. This have been related particularly in humans, whose specie in a decade, it was reported approximately thirty healthy babies born after procedures of cryopreservation and tranplant of ovarian tissue. However, the currently successful is due to hard efforts done in animals farm such as sheep. Reports of success after cryopreservation and transplantation of ovarian tissue in the early 90 stimulated several researchers to invest efforts and dedication in that research field, regarding human reproduction, and although success is evident, advances in cryopreservation of ovarian tissue in sheep and goats are still quite limited. Therefore, the aim of this work is to present a retrospective of the main results obtained with the cryopreservation of ovarian tissue in these two species.

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“…33,42,48,61 However, the outcome of oocyte cryopreservation has been largely dismal so far. 13,17,18,37,39,52 The two major biophysical events that may result in damage to oocytes during cryopreservation is the formation of a significant amount of ice inside the cells (intracellular ice formation or IIF) and excessive cell dehydration as a result of water transport out of the cells. 47,56 Conventionally, oocyte cryopreservation was done by the so-called slow-freezing approach which relies on the use of an optimal slow cooling rate (usually <2°C/min) to dehydrate the cells so that IIF can be minimized while, at the same time, without incurring significant cell damage by excessive cell dehydration.…”

Section: Introductionmentioning

confidence: 99%

Biotransport Phenomena in Freezing Mammalian Oocytes

Yang

Veres²,

Szalai³

et al. 2010

Ann Biomed Eng

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Water transport across the cell plasma membrane and intracellular ice formation (IIF)-the two biophysical events that may cause cell injury during cryopreservation-were studied by cryomicroscopy and modeling using mammalian (Peromyscus) oocytes. Unusually high activation energy for water transport across the cell plasma membrane was identified indicating that the water transport process is unusually sensitive to temperature (and cooling rate). Although literally all studies on IIF were conducted using protocols with ice-seeding (seeding extracellular ice usually at ≥-7 °C), it is not used for cell cryopreservation by vitrification that is becoming increasingly popular today. In this article, we show that ice-seeding has a significant impact on IIF. With ice-seeding and cooling at 60 °C/min, IIF was observed to occur over a wide range from approximately -8 to -48 °C with a clear change of the ice nucleation mechanism (from surface- to volume-catalyzed nucleation) at approximately -43 °C. On the contrary, without ice-seeding, IIF occurred over a much narrower range from approximately -19 to -27 °C without a noticeable change of the nucleation mechanism. Moreover, the kinetics of IIF without ice-seeding was found to be strongly temperature (and cooling rate) dependent. These findings indicate the importance of quantifying the IIF kinetics in the absence of ice-seeding during cooling for development of optimal vitrification protocols of cell cryopreservation.

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Cryopreservation of Human Oocytes and Ovarian Tissue

Cited by 52 publications

References 66 publications

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Advances in ovarian tissue cryopreservation in goats and sheep

Biotransport Phenomena in Freezing Mammalian Oocytes

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