Oocyte retrieval and selection

Rienzi, Laura; Ubaldi, Filippo Maria

doi:10.3109/9781841849713.007

Cited by 2 publications

(2 citation statements)

References 205 publications

(287 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Participants underwent a standard IVF using recombinant FSH (rFSH, Gonal-F; Serono, Geneva, Switzerland) on day 3 of menstrual cycle in combination with decapeptyl (DebioPharm, Geneva, Switzerland) or cetrorelix (Serono, Geneva, Switzerland) for controlled ovarian stimulation. 13 The dose of rFSH was chosen between 150 and 300 IU/d, depending on body mass index, age of the women, and the anticipated ovarian response. The ovarian response was monitored by follicular development on ultrasound and serum estradiol concentration every 2 days from stimulation days 6-7.…”

Section: Isolation Of Cumulus Cells and Gv Oocytesmentioning

confidence: 99%

See 1 more Smart Citation

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

et al. 2018

View full text Add to dashboard Cite

Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (-47%, P = .01), aromatase gene expression (-36%, P = .02), and estradiol production (-49%, P = .01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (-18%, P < .01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.

show abstract

Section: Isolation Of Cumulus Cells and Gv Oocytesmentioning

confidence: 99%

“…Oocytes were collected 36 hours after HCG administration by vaginal ultrasound-guided puncture of the ovarian follicles. 13…”

Section: Isolation Of Cumulus Cells and Gv Oocytesmentioning

confidence: 99%

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

et al. 2018

View full text Add to dashboard Cite

show abstract

Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone

Akdemir

Çakıl

Selam

et al. 2022

Life

View full text Add to dashboard Cite

Immature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.

show abstract

Oocyte retrieval and selection

Cited by 2 publications

References 205 publications

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone

Contact Info

Product

Resources

About