Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Fayezi, Shabnam; Novin, Marefat Ghaffari; Darabi, Masoud; Norouzian, Mohsen; Nouri, Mohammad; Farzadi, Laya; Darabi, Maryam

doi:10.1177/1933719117698578

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…Importantly, SCD activity is inhibited by SA in bovine cumulus cells of intact COCs, thus causing impaired oocyte development compared to denuded oocytes exposed to SA in the absence of cumulus cells [100]. SCD inhibition in human COCs not only leads to compromised oocyte development but also to reduced aromatase expression, which is followed by decreased E2 production [101].…”

Section: Unsaturated Fatty Acidsmentioning

confidence: 99%

Non-esterified fatty acids in the ovary: friends or foes?

Baddela

Sharma

Vanselow

2020

Reprod Biol Endocrinol

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A majority of common metabolic diseases can result in excessive lipolysis, leading to elevated levels of non-esterified fatty acids (NEFAs) in the body fluids. In females, increased NEFA levels in the follicular fluid markedly alter the functions of intrafollicular cells such as granulosa cells (GCs) and oocytes. Therefore, elevated levels of NEFAs have been suggested to be a significant player of subfertility in females of both human and economically important animal species such as cattle, buffalo, sheep, pig, chicken, and dog. However, the effects imposed by saturated and unsaturated fatty acids (SFAs and UFAs) on ovarian follicles are controversial. The present review emphasizes that SFAs induce apoptosis in granulosa and cumulus cells of ovarian follicles in different species. They further could adversely affect oocyte maturation and developmental competence. Many types of UFAs affect steroidogenesis and proliferation processes and could be detrimental for follicular cells, especially when at elevated concentrations. Interestingly, monounsaturated fatty acids (MUFAs) appear to contribute to the etiology of the polycystic ovarian syndrome (PCOS) as they were found to induce the transcription and translation of the androgenic transcription factor SOX9 while downregulating its estrogenic counterpart FOXL2 in GCs. Overall, this review presents our revised understanding of the effects of different fatty acids on the female reproductive success, which may allow other researchers and clinicians to investigate the mechanisms for treating metabolic stress-induced female infertility.

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Section: Unsaturated Fatty Acidsmentioning

confidence: 99%

Non-esterified fatty acids in the ovary: friends or foes?

Baddela

Sharma

Vanselow

2020

Reprod Biol Endocrinol

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“…Furthermore, when SCD activity in cumulus cells was inhibited during exposure to a physiological level of stearic acid, the developmental competence of the oocyte was hampered and comparable with the situation where oocytes were exposed to stearic acid in the absence of cumulus cells (Aardema et al, 2017). In a recent paper, the negative outcome of SCD inhibition in cumulus cells on oocyte developmental competence was also confirmed in human cumulus cells and also appeared to relate to reduced aromatase gene expression and estradiol production (Fayezi et al, 2018). SCD-1 expression has been associated with lipid metabolism in dairy cows and may be linked to reproductive performance (Wathes et al, 2012).…”

Section: Cumulus Cells Protect the Oocyte Against Potential Stress Bymentioning

confidence: 89%

Cumulus cells protect the oocyte against saturated free fatty acids

Aardema¹,

Vos²,

Gadella³

2018

Anim Reprod

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In the cow a major characteristic of metabolic stress is an elevated level of plasma free fatty acid, due to increased lipid mobilization from adipose tissue. Elevated levels of free fatty acids in blood (complexed to albumin) are associated with increased lipotoxicity in non-adipose tissue. An overview is provided on the negative impact of free fatty acids and the metabolic stress imposed on the oocyte and early embryo and thus on bovine fertility. There is increasing evidence that in vitro as well as in vivo the elevated levels of free fatty acids in blood during metabolic stress can severely hamper oocyte and embryo development. However, fatty acids do also form an essential nutrient source for the oocyte and embryo, which indicates that these good and bad effects of fatty acids should be in subtle balance to optimize the developmental competence of the oocyte and embryo.

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“…3). Endogenous synthesis of OlAc is primarily regulated by stearoyl-coenzyme A desaturase (SCD) in multiple cell types, including follicular cells (Feuerstein et al ., 2007; Aardema et al ., 2017; Fayezi et al ., 2017). A recent study showed that prepubertal heifers versus cows had higher levels of SCD gene expression in granulosa cells and OlAc in follicular fluid (Warzych et al ., 2017).…”

Section: Oleic Acid In Endocrine-related Reproduction Functionmentioning

confidence: 99%

Oleic acid in the modulation of oocyte and preimplantation embryo development

Fayezi

Leroy

Novin

et al. 2017

Zygote

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Potential reproductive effects are considered as the major aspect of biomolecules functionality in an organism. The recent identification of differential patterns of fatty acids across ovarian follicles and their association with levels of sexual maturity highlights the importance of these biomolecules. It is well known that fatty acids are highly diverse in terms of their functional properties. Oleic acid is chemically classified as an unsaturated omega-9 fatty acid. Besides serving as an important energy source, oleic acid is involved in metabolic and structural roles. Free and esterified oleic acids are compartmentalized into discrete extracellular fluids, cell organelles and found within the cytosol. This review summarizes the current knowledge on the contribution of oleic acid in regulating female fertility, particularly its involvement in female germ cell growth and development. Oleic acid has been identified as a blastomeric and post-cryopreservation survival biomarker in bovine. Several related studies have shown the critical role of oleic acid in counteracting the detrimental effects of saturated fatty acids and in paracrine support of oocyte development. Although available data are not ideally detailed, most data suggest that oleic acid can contribute to normal oocyte and preimplantation embryo development via mechanisms involving metabolic partitioning of fatty acids, change in the membrane structural organization, attenuation of oxidative stress and regulation of intracellular signalling. Thus, oleic acid may play a significant role in oocyte and early embryo development, suggesting that future studies should explore in more detail its potential effects on the physiopathology of female reproduction.

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Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Cited by 17 publications

References 33 publications

Non-esterified fatty acids in the ovary: friends or foes?

Non-esterified fatty acids in the ovary: friends or foes?

Cumulus cells protect the oocyte against saturated free fatty acids

Oleic acid in the modulation of oocyte and preimplantation embryo development

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