Oocyte-derived Smad4 is not required for development of the oocyte or the preimplantation embryo

Kaune, Heidy; Peyrache, Emeline; Williams, Suzannah A.

doi:10.1016/j.theriogenology.2014.11.024

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…However, the exact role in the oocytes was not fully elucidated. [22]. The fall of SMAD4 expression in the oocyte might be associated with transfer to the granulosa cell devoid environment.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study

Chermuła

Brązert

Ješeta

et al. 2019

IJMS

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This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the “transforming growth factor β receptor signaling pathway”, “response to protein stimulus”, and “response to organic substance” were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study

Chermuła

Brązert

Ješeta

et al. 2019

IJMS

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“…They used mutant females with oocyte-specific SMAD4 deletion and control mice and mated them with control males. Examination showed no reduction in fertility nor litter size [11].…”

Section: Discussionmentioning

confidence: 88%

“…The generation of fully competent egg requires interactions between the oocyte and ovary, and TGFB was found to be major signaling pathway. Granulosa cells deprived of SMAD4 interrupt follicle growth, however its role in the oocyte has not been determined so far [11]. There are some information about TGFB signaling pathway involvement in sexual determination of germ cells.…”

Section: Discussionmentioning

confidence: 99%

Does Porcine Oocytes Maturation in Vitro is Regulated by Genes Involved in Transforming Growth Factor Beta Receptor Signaling Pathway?

Budna

Celichowski

Karimi

et al. 2017

Advances in Cell Biology

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Summary:The oocyte growth and development in follicular environment are substantially accompanied by surrounding somatic cumulus (CCs) and granulosa cells (GCs). During these processes, the mammalian gametes reach full maturational stage and may be further successfully fertilized by single spermatozoon. These unique mechanisms are regulated by expression of clusters of genes and their biochemical signaling pathways. In this article we described differential expression pattern of transforming growth factor beta (TGFB) gene superfamily in porcine oocytes before and after in vitro maturation (IVM). We performed Affymetrix® microarray assays to investigate the TGFB-related genes expression profile in porcine immature oocytes and gametes cultured for 44h in vitro. In results we found 419 different genes, 379 genes with lower expression, and 40 genes characterized by increased RNA profile. Moreover, significant up-regulation of 6 genes belonging to TGFB signaling pathway such as: TGFBR3, SMAD4, FOS, KLF10, ID1, MAP3K1 in immature porcine oocytes (before IVM), was also observed. It may be suggested that genes involved in TGFB-related signaling pathway are substantially regulated before IVM. Furthermore, these genes may play a significant role during early stages of nuclear and/or cytoplasmic porcine oocytes maturation. The investigated transcripts may be also recommended as the markers of oocytes maturational capability in pigs.

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“…In the comparison of oocytes with expanded cumulus cells and those with compact cumulus cells, expression of STAT3 was detected in oocytes associated with high developmental competence [13]. Although it was reported that the deletion of SMAD4 using Gdf9-icre results in a slight but significant reduction in litter size in mice [14], the weaker contribution of this factor to bovine oocyte development was also reported [15]. Here, the expression level of SMAD4 was high during follicular development and gradually but significantly increased from EAFs to LAFs (RPKM values of EAFs, SAFs, and LAFs, are 6720, 6885, and 7473, respectively).…”

Section: Discussionmentioning

confidence: 99%

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

Munakata

Kawahara-Miki

Shiratsuki

et al. 2016

Journal of Reproduction and Development

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Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it is important to understand the processes that occur in oocytes and granulosa cells during follicle development and the differences between in vivo and in vitro follicle development. In the present study, oocytes and granulosa cells were collected from early antral follicles (EAFs, 0.5–0.7 mm in diameter), small antral follicles (SAFs, 1–3 mm in diameter), large antral follicles (LAFs, 3–7 mm in diameter), and in vitro grown oocyte-and-granulosa cell complexes (OGCs), which were cultured for 14 days after collection from EAFs. Gene expression was analyzed comprehensively using the next-generation sequencing technology. We found top upstream regulators during the in vivo follicle development and compared them with those in in vitro developed OGCs. The comparison revealed that HIF1 is among the top regulators during both in vivo and in vitro development of OGCs. In addition, we found that HIF1-mediated upregulation of glycolysis in granulosa cells is important for the growth of OGCs, but the cellular metabolism differs between in vitro and in vivo grown OGCs. Furthermore, on the basis of comparison of upstream regulators between in vivo and in vitro development of OGCs, we believe that low expression levels of FLT1 (VEGFA receptor), SPP1, and PCSK6 can be considered causal factors of the suboptimal development under in vitro culture conditions.

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Oocyte-derived Smad4 is not required for development of the oocyte or the preimplantation embryo

Cited by 6 publications

References 35 publications

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study

Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study

Does Porcine Oocytes Maturation in Vitro is Regulated by Genes Involved in Transforming Growth Factor Beta Receptor Signaling Pathway?

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

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