Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet

Abstract

The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P<0.05) embryo production 21.98±2.37 vs 13.12±1.59). No difference was observed in serum Se concentration between the two groups. It is recommended that the daily diet be supplemented with 100g mineral salt containing 9.6mg Se, since this rate rendered a larger production of oocytes, higher quantity of degree 1 oocytes and greater production of embryos in the process of in vitro fertilization.Keywords: cattle dairy, antioxidant, glutathione peroxidase, in vitro fertilization, oocyte quality O total de oócitos, 35,11±2,65 vs 23,10±2,16, e oócitos de qualidade 1, 11,61±1,58 vs 4,75±0,97, foram mais elevados no grupo que recebeu 9,6mg de Se e a quantidade de oócitos desnudos mais baixa, 3,21±0,87 vs 6,22±1,18. A produção de embriões foi maior no grupo tratado com 9,6mg de Se/vaca/dia, 21,98±2,37 vs 13,12±1,59 RESUMO Avaliou-se o efeito do selênio (Se) adicional na dieta de vacas Jersey na aspiração de oócitos e produção de embriões in vitro. Dez vacas Jerseys receberam 3,2mg de Se por dia ou 9,6mg, vinculado ao concentrado. Realizaram-se seis aspirações foliculares, com intervalo médio de 15 dias, aproveitando as cinco últimas. Os oócitos foram classificados e realizaram-se os procedimentos padrões de maturação, fertilização e cultivo in vitro.

Effect of Selenium Supplementation on Twinning Induction in Barki Ewes

“…On the same hand, in vitro oocyte maturation and fertilization, Hirotada et al (2017) stated that Se overcame the undesirable oxidative stress produced by hydrogen peroxide, increased blastocyst formation and enhanced the development of pre-implanted mouse embryo of mouse blastocysts cultured in the presence of Se. Also, Moraes et al (2012) stated that dairy Jersey cows' diet supplemented with Se rendered the production of higher quantity of grade 1 oocytes and greater production of embryos in the process of in vitro fertilization.…”

Fatores Que Afetam a Produção in Vitro De Embriões (Pive) Em Bovinos

“…Outro fator de grande relevância, mas com poucos estudos, refere-se ao uso de antioxidantes na dieta das doadoras. Foi relatado que o selênio, quando adicionado a dieta de vacas em programa de PIVE promove maior quantidade de oócitos totais aspirados, viáveis, embriões e blastocistos expandidos, melhorando assim quantitativa e qualitativamente os resultados (MORAES et al, 2012).…”