Ontogeny of rat gastric H+-K+-ATPase activity

Hervatin, Florence; Moreau, Evelyne; Ducroc, Robert; Garzon, B.; Avril, P; Millet, P; Geloso, J. P.

doi:10.1152/ajpgi.1987.252.1.g28

Cited by 18 publications

(17 citation statements)

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“…DPC, days post-coitum; DPP, days post-partum. * not significantly different from zero (P < 0 05) stimulated active secretion of H+ were minimal , and that the activity of gastric H+, K+-ATPase had disappeared at that stage of development (Hervatin et al 1987). These earlier findings, together with the present results, indicate that chloride secretion, like H+ secretion, is abolished across the resting stomach by post-natal day 12.…”

Section: Discussionsupporting

confidence: 66%

“…gastrin (Takeuchi et al 1981;Garzon et al 1982) and histamine (Takeuchi et al 1981;Ackerman, 1982;Avril et al 1986). The activity of gastric H+, K+-ATPase, the acid pump involved in the terminal step of the acid secretion process (Sachs, Berglindh, Faller, Rabon, Malinowska, Cuppoletti, Gunther & Smolka, 1984) also decreases after day 5 of life and drops to its minimum on day 12 (Hervatin, Moreau, Ducroc, Garzon, Avril, Millet & Geloso, 1987).…”

Section: Introductionmentioning

confidence: 99%

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Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Ducroc

Garzon

Geloso

et al. 1988

The Journal of Physiology

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3. However, on fetal day 20, neutral absorption of NaCl was no longer observed but amiloride had inhibited electrogenic absorption of Na+, and significant active secretion of Cl-was observed (J" = -1-3 + 0-6 pequiv cm-2 h-1). On day 21 (i.e. 24 h before birth), values for JNa )Jclt, and Is were not different from those determined on adult gastric mucosa.4. After birth, NaCl transport continued to exhibit its prenatal characteristics on day 5 but not on day 12, when Na+ and Cl-were both absorbed; on that day, jNa -I was equal to both Jclt and to the amiloride-insensitive component of Isc, indicating that neutral NaCl absorption had resumed.5. These data show that in rat stomach, NaCl transport differentiates on fetal day 20, when H+ secretion is first observed, and thereafter undergoes biphasic development.6. The significant Cl-absorption observed on post-natal day 12 was concomitant with the inhibition of net H+ secretion.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Ducroc

Garzon

Geloso

et al. 1988

The Journal of Physiology

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“…In addition or alternatively, the developmental changes in acid secretion may result from changes in parietal cell function, probably in the H+-K+-ATPase activity (the functional H+ transporter in parietal cells). This activity appears to be rate limiting for the gastric H+ secretory process in developing rats and sheep and is stimulated by exogenous glucocorticoid treatment (Hervatin, Moreau, Ducroc, Garzon, Avril, Millet & Geleso, 1987;Hervatin et al 1989;Read, Chick, Hardy & Shulkes, 1992).…”

Section: Discussionmentioning

confidence: 99%

Secretion of acid, gastrin, and cobalamin‐binding proteins by the fetal pig stomach: developmental regulation by cortisol

Sangild¹,

Hilsted²,

Nexø³

et al. 1994

Experimental Physiology

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SUMMARYThe role of cortisol in the development of gastric function was investigated in the fetal pig. Pregnant sows (term = 1 14 + 2 days, n = 10) were anaesthetized (pentobarbitone) and osmotic minipumps containing either saline or cortisol inserted subcutaneously into fetuses at 82-84 (n = 11) or 91-96 (n = 14) days gestation. Six days later the infused pigs were removed by Caesarean section and the stomach and its contents collected for analysis. Samples were also obtained from unoperated (control) fetal pigs (n = 51) removed under anaesthesia at intervals from 70 days gestation until term. The concentrations of acid in gastric fluid, gastrin in plasma and antral tissue (bioactive as well as precursor forms), and cobalamin(CBL)-binding proteins in fundic tissue were low in control pigs until 100 days gestation. During the last 2 weeks before term, large increases occurred in gastric fluid acidity (from pH 6-7 to pH 3 at birth), plasma amidated gastrin (from 10 to 90 pM) and CBL-binding protein concentration (from 10 to 80 pmol (g fundus)-1). These changes occurred in parallel with a surge in plasma cortisol (from 20 to 200 ng ml-'). Immature fetuses infused with cortisol had significantly higher gastric acidity, plasma amidated gastrin, and concentration of CBL-binding proteins than the corresponding control fetuses (P < 0 05). Across age and treatment groups, significant correlations were found between log1o plasma cortisol values and the above parameters (r = 0 59-076, P < 001). The results suggest that endogenous cortisol secretion stimulates the secretion of gastric acid, gastrin and CBL-binding proteins in the prenatal period of the pig.

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“…The increased expression of cytokeratin from gestational day 16 to 18 shows that the epithelial character of these embryonic cells becomes more distinct during this period. According to Hervatin et al (1987) the parietal cell marker H,K-ATPase was not detected until gestational day 19 in the rat. In agreement with this observation we could not detect the enzyme at gestational day 18.…”

Section: Discussionmentioning

confidence: 99%

Expression of extracellular matrix proteins in the fetal rat gastric mucosa

Tømmerås¹,

Cabero

Mårdh³

2000

Anat Embryol

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At gestational day 16 the epithelium of the rat stomach consists of a stratified layer of undifferentiated cells, and two days later glandular structures appear. The present study was carried out to identify extracellular matrix proteins that could be involved in the epithelial cell proliferation and differentiation processes that occur in the fetal rat stomach during this period. For comparative purposes the expression of the same components in the adult gastric mucosa was examined. Pregnant Sprague-Dawley rats received an intraperitoneal injection of 5-bromo-2'-deoxyuridine to label proliferating cells. One, 3.5, or 6 h post-injection the stomachs were excised and immediately frozen. The specimens were sectioned and stained with hematoxylin and eosin or for 5-bromo-2'-deoxyuridine, cytokeratin no. 8, H,K-ATPase, and the extracellular matrix proteins fibronectin, laminin, and collagens type I and IV. A stratified layer of proliferating cells was observed in the epithelium of the fetal stomachs, while in adult stomachs proliferating cells were detected in the isthmus/neck region of the glands. Cytokeratin, an epithelial cell marker, was sparse at gestational day 16 but abundant both at gestational day 18 and in the isthmus/neck region of gastric glands of the adult stomach. The parietal cell marker H,K-ATPase could not be detected in the fetal stomachs during this period. Fibronectin was observed in the stroma of both fetal and adult stomachs. Collagen type I could only be detected in the stroma close to the oesophagus at gestational day 16. Two days later, collagen type I was abundant in the lamina propria, the submucosa and in the serosa of the fetal stomachs. In adult tissue collagen type I was detected in the surface epithelium, the submucosa and in the serosa of the stomach. Collagen type IV and laminin were expressed in the lamina propria, the basement membranes around blood vessels, muscle cells, and nerve bundles, as well as in the serosa of both 16- and 18-day-old fetal and adult rat stomachs. In conclusion, a high cell proliferation rate was observed in the epithelium at both gestational days 16 and 18. The increased expression of cytokeratin observed during this period indicates that the epithelial character of the embryonic cells becomes more distinct, while the remarkable change in the expression of collagen type I might reflect an important role of collagen type I in the development of the gastric epithelium.

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Ontogeny of rat gastric H+-K+-ATPase activity

Cited by 18 publications

References 0 publications

Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Secretion of acid, gastrin, and cobalamin‐binding proteins by the fetal pig stomach: developmental regulation by cortisol

Expression of extracellular matrix proteins in the fetal rat gastric mucosa

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