Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Ducroc, Robert; Garzon, B.; Geloso, J. P.; Hervatin, Florence; Millet, P; Moreau, Evelyne

doi:10.1113/jphysiol.1988.sp017056

Cited by 5 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gallbladder was opened, rinsed with Ringer solution and vertically mounted as flat sheets in Ussingtype chambers [8] (exposed surface area=0.14 or 0.22 cm 2). A circle of high-viscosity silicone gel (Bayer) was applied to the inner rim of the interface between the two chamber halves to minimize edge damage.…”

Section: Study Of Na+ Fluxesmentioning

confidence: 99%

Role of the NHE3 isoform of the Na+/H+ exchanger in sodium absorption by the rabbit gallbladder

Silviani

Colombani

Heyries

et al. 1996

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

The absorption of water and electrolytes by the gallbladder seems to be largely dependent upon a Na+/H+ exchange at the apical membrane of the gallbladder epithelium. To find out if the exchanger involved is the NHE3 isoform, as in other absorbing epithelia, two studies were performed using the rabbit gallbladder. First, we studied 22Na absorption in Ussing chambers with Krebs buffer as a control solution, and in the presence of amiloride (100, 200 or 1000 microM), ethyl-isopropyl-amiloride (EIPA, 1 or 5 microM), or the phorbol ester, phorbol 12-myristate 13-acetate (PMA, 1 microM). A net mucosal-to-serosal Na+ flux was observed with control buffer. No inhibition of this net flux was observed with 5 microM EIPA, and the IC50 for amiloride was found to be 200 microM. PMA induced a reduction of absorption by 30% that was prevented by incubation with calphostin C. Resistance to amiloride and EIPA, and inhibition by PMA are consistent with the involvement of the NHE3 isoform. The second study involved reverse-transcriptase polymerase chain reaction (RT-PCR) of total gallbladder RNA, with two primers designed to amplify a 645-base-pair fragment from NHE3 mRNA. A cDNA fragment of the expected size was actually obtained from gallbladder RNA, while RT-PCR of RNA from the liver, which does not contain NHE3, gave negative results. A sequence of 492 nucleotides of the amplified product was determined, which was almost superimposable onto the known sequence of the corresponding fragment of rabbit NHE3. It is concluded that, in rabbit gallbladder, neutral NaCl absorption is, at least in part, dependent on the NHE3 isoform of the Na+/H+ exchanger.

show abstract

Section: Study Of Na+ Fluxesmentioning

confidence: 99%

Role of the NHE3 isoform of the Na+/H+ exchanger in sodium absorption by the rabbit gallbladder

Silviani

Colombani

Heyries

et al. 1996

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…Tissue conductance and permeability measurements Pieces of colon (0.5 cm 2 ) were mounted in Ussing chambers as previously described (Ducroc et al, 1988). Electrogenic ion transport was monitored as short-circuit current (Isc) using an …”

Section: Image Acquisition and Analysesmentioning

confidence: 99%

Early colonizing Esche richia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state

Tomas¹,

Reygner²,

Mayeur³

et al. 2014

The ISME Journal

View full text Add to dashboard Cite

We investigated the effects of early colonizing bacteria on the colonic epithelium. We isolated dominant bacteria, Escherichia coli, Enterococcus faecalis, Lactobacillus intestinalis, Clostridium innocuum and a novel Fusobacterium spp., from the intestinal contents of conventional suckling rats and transferred them in different combinations into germfree (GF) adult rats. Animals were investigated after various times up to 21 days. Proliferative cell markers (Ki67, proliferating cell nuclear antigen, phospho-histone H3, cyclin A) were higher in rats monocolonized with E. coli than in GF at all time points, but not in rats monocolonized with E. faecalis. The mucin content of goblet cells declined shortly after E. coli administration whereas the mucus layer doubled in thickness. Fluorescence in situ hybridization analyses revealed that E. coli resides in this mucus layer. The epithelial mucin content progressively returned to baseline, following an increase in KLF4 and in the cell cycle arrest-related proteins p21 CIP1 and p27 KIP1. Markers of colonic differentiated cells involved in electrolyte (carbonic anhydrase II and slc26A3) and water (aquaglyceroporin3 (aqp3)) transport, and secretory responses to carbachol were modulated after E. coli inoculation suggesting that ion transport dynamics were also affected. The colonic responses to simplified microbiotas differed substantially according to whether or not E. coli was combined with the other four bacteria. Thus, proliferation markers increased substantially when E. coli was in the mix, but very much less when it was absent. This work demonstrates that a pioneer strain of E. coli elicits sequential epithelial remodeling affecting the structure, mucus layer and ionic movements and suggests this can result in a microbiota-compliant state.

show abstract

“…We previously demonstrated that in the rat, the development of H '" and CI -secretion was characterized by a fetal phase of emergence and maturation, followed by a postnatal phase of reduced secretion (1-4). By day 12 of life, the basal secretion of H + and CI-ions, as with the activity of gastric H + , K + -ATPase, dropped to zero (4)(5)(6), and the parietal cell was still insensitive to exogenous histamine (5). Since thi s gradual inhibition of acid secretion and reduced responsiveness to histamine were observed after 5 days of suckling (5,6), we postulated that a short-term milk diet might interfere with the histaminergic regulation of acid secretion.…”

Section: Inhibition By Cow's Milk Of Histamine-induced Gastric Acid S...mentioning

confidence: 99%

Inhibition by Cow's Milk of Histamine‐Induced Gastric Acid Secretion in the Rat

Wirbel,

Ducroc,

Garzon

et al. 1989

J. pediatr. gastroenterol. nutr.

View full text Add to dashboard Cite

The effects of a 4‐day milk diet on basal and histamine‐induced gastric acid secretion were examined in vivo in adult rats. Rats were fed on raw cow's milk only. Their acid secretion was measured in the perfused stomach under basal conditions or after administration of varying doses of histamine. In milk‐fed rats, basal secretion rose slightly compared with control secretion (1.86 ± 0.09 vs. 1.52 ± 0.06 μEq HV+/10min; p > 0.01), but maximal secretion in response to noncumulative doses of histamine (range: 7.0–20.0 mg/kg) was inhibited by 57%. The degree of this inhibition was similar to that observed in control rats with an equimolar dose of H2‐antagonist cimetidine. These results indicate that in the adult rat, chronic administration of cow's milk strongly inhibits his‐tamine‐stimulated acid output.

show abstract

Development of sodium and chloride transport across fetal and newborn rat stomach in vitro.

Cited by 5 publications

References 17 publications

Role of the NHE3 isoform of the Na+/H+ exchanger in sodium absorption by the rabbit gallbladder

Role of the NHE3 isoform of the Na+/H+ exchanger in sodium absorption by the rabbit gallbladder

Early colonizing Esche richia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state

Inhibition by Cow's Milk of Histamine‐Induced Gastric Acid Secretion in the Rat

Contact Info

Product

Resources

About