Ontogenetic variation in cold tolerance plasticity in Drosophila: is the Bogert effect bogus?

Mitchell, Katherine A.; Sinclair, Brent J.; Terblanche, John S.

doi:10.1007/s00114-013-1023-8

Cited by 27 publications

(28 citation statements)

References 19 publications

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“…Consistent with past studies [10], [20], adults and pupae were clearly hardier than embryos and larvae. The death and necrosis of larvae, particularly in the third instar, suggested strongly that a healthy stock could best be maintained by separating pupae or adults from larvae during extended intervals of cold storage.…”

Section: Discussionsupporting

confidence: 91%

Effect of Prolonged Coldness on Survival and Fertility of Drosophila melanogaster

Mockett

Matsumoto

2014

PLoS ONE

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The laboratory fruit fly, Drosophila melanogaster, is used widely in biological research, but the requirement to maintain stocks with a roughly biweekly generation time imposes substantial burdens of labor, potential cross-contamination and mutation accumulation. The purpose of this study was to assess the impact of prolonged cold stress or milder cooling on survivorship and fertility. The hypothesis was that cold storage would result in postponement of reproduction and a longer generation time. Flies of several genotypes were maintained continuously at 4–11°C; recovery rates and subsequent yields of adult progeny were recorded. Adults and pupae of a relatively long-lived y w lineage were more resistant to severe cold stress than embryos and larvae. Adults exhibited minimal mortality up to at least 5 d at 4°C, 20 d at 8°C and 12 weeks at 11°C. Reproduction did not occur at these temperatures, but progeny were obtained after recovery at 25°C. At all temperatures, chilling caused a rapid, severe and progressive decrease in fertility during the first 2 d of recovery. The impact on fertility during the subsequent 2–4 d was much milder and it occurred only after prolonged incubation at low temperatures. The total reproductive output during the first 6 d of recovery was sufficient to replace the parental population after 12 weeks at 11°C. Food spoilage had an unexpectedly low impact on survivorship and fertility, and the reproductive output of F1 progeny was not affected by storing parental flies at 11°C for 8–10 weeks. In the case of w 1118 flies, replacement of the parents within 6 d of recovery was possible for up to 60 d at 11°C. Among less fertile genotypes, replacement of the parents was possible within 18 d after 4–10 weeks at 11°C. These results show that the 2-week maintenance interval of stocks of D. melanogaster can be extended 3–7 fold, at least for 1 generation, by storing adult flies at 11°C.

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Section: Discussionsupporting

confidence: 91%

Effect of Prolonged Coldness on Survival and Fertility of Drosophila melanogaster

Mockett

Matsumoto

2014

PLoS ONE

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“…The high heat tolerance of pupae might be explained by Bogert effect (Huey, Hertz & Sinervo, 2003). According to this principle, behavioral thermoregulation allows ectothermic animals to escape lethal temperatures, thus less mobile stages (like pupae) have to cope with and tolerate more extremes conditions than mobile stages (Marais & Chown, 2008; Mitchell, Sinclair & Terblanche, 2013). …”

Section: Discussionmentioning

confidence: 99%

Basal tolerance to heat and cold exposure of the spotted wing drosophila,Drosophila suzukii

Enriquez

Colinet

2017

PeerJ

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The spotted wing Drosophila, Drosophila suzukii, is a new pest in Europe and America which causes severe damages, mostly to stone fruit crops. Temperature and humidity are among the most important abiotic factors governing insect development and fitness. In many situations, temperature can become stressful thus compromising survival. The ability to cope with thermal stress depends on basal level of thermal tolerance. Basic knowledge on temperature-dependent mortality of D. suzukii is essential to facilitate management of this pest. The objective of the present study was to investigate D. suzukii basal cold and heat tolerance. Adults and pupae were subjected to six low temperatures (−5–7.5 °C) and seven high temperatures (30–37 °C) for various durations, and survival-time-temperature relationships were investigated. Data showed that males were globally more cold tolerant than females. At temperature above 5 °C, adult cold mortality became minor even after prolonged exposures (e.g., only 20% mortality after one month at 7.5 °C). Heat tolerance of males was lower than that of females at the highest tested temperatures (34, 35 and 37 °C). Pupae appeared much less cold tolerant than adults at all temperatures (e.g., Lt50 at 5° C: 4–5 d for adults vs. 21 h for pupae). Pupae were more heat tolerant than adults at the most extreme high temperatures (e.g., Lt50 at 37 °C: 30 min for adults vs. 4 h for pupae). The pupal thermal tolerance was further investigated under low vs. high humidity. Low relative humidity did not affect pupal cold survival, but it reduced survival under heat stress. Overall, this study shows that survival of D. suzukii under heat and cold conditions can vary with stress intensity, duration, humidity, sex and stage, and the methodological approach used here, which was based on thermal tolerance landscapes, provides a comprehensive description of D. suzukiithermal tolerance and limits.

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“…For instance, if indeed stress resistance and plasticity therein matters most, do these traits differ throughout ontogeny? If so, as has been found in at least some cases (Bowler & Terblanche, ; Mitchell et al ., ; Radchuk et al ., ; Klockmann et al ., ), resistance in which developmental stage is most relevant? Also, species may show cross‐resistance, that is, cold‐adapted species may actually show at the same time high resistance to other stressors including heat (Burton et al ., ; Nielsen et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Variation in adult stress resistance does not explain vulnerability to climate change in copper butterflies

2017

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Ongoing climate change is a major threat to biodiversity. However, although many species clearly suffer from ongoing climate change, others benefit from it, for example, by showing range expansions. However, which specific features determine a species' vulnerability to climate change? Phenotypic plasticity, which has been described as the first line of defence against environmental change, may be of utmost importance here. Against this background, we here compare plasticity in stress tolerance in 3 copper butterfly species, which differ arguably in their vulnerability to climate change. Specifically, we investigated heat, cold and desiccation resistance after acclimatization to different temperatures in the adult stage. We demonstrate that acclimation at a higher temperature increased heat but decreased cold tolerance and desiccation resistance. Contrary to our predictions, species did not show pronounced variation in stress resistance, though plastic capacities in temperature stress resistance did vary across species. Overall, our results seemed to reflect population-rather than species-specific patterns. We conclude that the geographical origin of the populations used should be considered even in comparative studies. However, our results suggest that, in the 3 species studied here, vulnerability to climate change is not in the first place determined by stress resistance in the adult stage. As entomological studies focus all too often on adults only, we argue that more research effort should be dedicated to other developmental stages when trying to understand insect responses to environmental change.

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Ontogenetic variation in cold tolerance plasticity in Drosophila: is the Bogert effect bogus?

Cited by 27 publications

References 19 publications

Effect of Prolonged Coldness on Survival and Fertility of Drosophila melanogaster

Effect of Prolonged Coldness on Survival and Fertility of Drosophila melanogaster

Basal tolerance to heat and cold exposure of the spotted wing drosophila,Drosophila suzukii

Variation in adult stress resistance does not explain vulnerability to climate change in copper butterflies

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