Online Training for Silage Feed Production in Nggorang Village

Detha, Annytha Ina Rohi; Ndaong, Nemay; Foeh, Nancy; Maranatha, Grace; Datta, Frans Umbu

doi:10.23887/ijcsl.v5i4.40305

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…Goat milk was processed using cold centrifuged (4 o C) at 1,280 × g for about 30 minutes to separate its fat (skimmed milk). Casein fraction and whey from skimmed goat milk are separated based on method from Detha et al, (2013) with modification. Skim goat milk is slowly acidified with HCl 2 N until pH 4.2.…”

Section: Preparation Of Milkmentioning

confidence: 99%

Antibacterial Activity From Goat’s Milk Whey Hydrolized by Protease Enzyme of Bacillus Lincheniformis

Arief

Rahmadhina

Budiman

2023

JITEK

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Food additives had been used to prevent and inhibit the growth of microorganisms and extend shelf life of food. Based on the safety of their used, it has an impact such as microorganism resistance to synthetic antimicrobials. This encourages the development of antimicrobials derived from natural ingredients, especially from milk. The bioactive components of milk protein have not always existed in their natural state, including precursors or peptides that will only be active if they have been hydrolyzed in vitro from their natural proteins. Hydrolysis of milk protein can be carried out using proteolytic enzymes from various sources, including protease extracted from Bacillus lincheniformis. This study used different concentrations of the protease extracted from Bacillus lincheniformis (0.5%, 1% and 1.5%) to produce hydrolyzate which has antibacterial activity in terms of protein content, SDS PAGE profile and the size of the inhibition zone (disc method). The results showed that the best activity of the whey hydrolyzed on Gram-negative Escherichia coli was 1% concentration of protease enzyme. In comparison, Gram positive Staphylococcus aureus can be inhibited with 0.5% concentration of protease enzyme.

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Section: Preparation Of Milkmentioning

confidence: 99%