Onion-like carbon re-inforced electrospun polyacrylonitrile fibres for ultrasensitive electrochemical immunosensing of Vibrio cholerae toxin

Ozoemena, Okoroike C.; Mathebula, Nsovo S.; Ehirim, Tobechukwu J.; Maphumulo, Tobile; Valikpe, Goodness M.; Shai, Jerry L.; Ozoemena, Kenneth I.

doi:10.1016/j.electacta.2020.136816

Cited by 9 publications

(14 citation statements)

References 34 publications

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“…The bare GCE (Figure 3d) showed lower electron transport (Δ E p ) compared to the other modified electrodes: GCE (Δ E p =20 mV and I pa =6.40 μA), f‐MWCNTs‐GCE (Δ E p =50 mV and I pa =34.28 μA) ZnONPs‐f‐MWCNTs‐GCE (Δ E p =40 mV and I pa =59.65 μA) and IL‐ZnONPs‐f‐MWCNTs‐GCE (Δ E p =20 mV and I pa =116.42 μA) (Table 1). The IL‐f‐MWCNTs networks provide outstanding support for ZnONPs with largely exposed surface area [34]. In addition, the f‐MWCNTs facilitated good electronic pathways for the electron transfer which also significantly enhanced the electrocatalysis.…”

Section: Resultsmentioning

confidence: 97%

“…The IL-f-MWCNTs networks provide outstanding support for ZnONPs with largely exposed surface area E pa = anodic peak potential, E pc = cathodic peak potential, I pa = anodic peak current, I pc = cathodic peak current and ΔE p = peak potential separation. [34]. In addition, the f-MWCNTs facilitated good electronic pathways for the electron transfer which also significantly enhanced the electrocatalysis.…”

Section: Influence Of Scan Ratementioning

confidence: 94%

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Ionic Liquid and f‐MWCNTs Fabricated Glassy Carbon Electrode for Determination of Amygdalin in Apple Seeds

Chokkareddy

2020

Electroanalysis

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This work proposes a novel electrochemical sensor based on functionalised multiwalled carbon nanotubes (f-MWCNTs), zinc oxide nanoparticles (ZnONPs), and (methyltrioctylammonium chloride ionic liquid (IL)) fabricated glassy carbon electrode (GCE), for the determination of amygdalin (AMY) in apple seeds. X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier-transform infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Thermogravimetric analysis (TGA) as well as electrochemical impedance spectroscopy (EIS) techniques were used to characterize the synthesized ZnONPs and its nanocomposite. EIS and differential Pulse Voltammetry (DPV) was utilized to investigate the electrochemical behavior of AMY on the IL-ZnONPs-f-MWCNTs nanocomposite modified GCE. The linear range response is obtained from 0.012-6 μM for AMY determination under optimized experimental conditions, and the limit of detection (LOD) values were found to be 0.012 μM. The novel method developed was successfully used to determine AMY in spiked apple seed samples with satisfactory results.

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Section: Resultsmentioning

confidence: 97%

Section: Influence Of Scan Ratementioning

confidence: 94%

Ionic Liquid and f‐MWCNTs Fabricated Glassy Carbon Electrode for Determination of Amygdalin in Apple Seeds

Chokkareddy

2020

Electroanalysis

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“…For example, culture method can take up to 8 days to get the results while, according to literature [4], cholera kills within 12 to 24 h if not treated. There are many other methods such as enzyme-linked immunosorbent assay [5], polymerase chain reaction (PCR) [6,7], and electrochemical methods [8][9][10][11][12][13][14][15]. Of all the aforementioned techniques, electrochemistry gives excellent promise for fast detection, simplicity, sensitivity, selectivity, and ability to miniaturize the process for the development of hand-held point-of-care diagnostic devices.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, several researchers have reported several electrode platforms for the immobilization of cholera immunosensors. These electrode platforms include graphene [8], liposomes and poly (3,4ethylenedioxythiophene)-coated carbon nanotubes [9], dendrimers integrated with gold nanoparticles [10], zinc oxide [11], polyacrylonitrile (PAN) nanofibres [12], copper (II) complex with polypyrrole-nitrilotriacetic acid on carbon nanotubes [13], carbon nanofibres (CNF) [14], and onionlike carbon modified with PAN (OLC-PAN) [15]. In electrochemistry, to be redox-active, a material must be able to lose or gain electrons (i.e., conducting), while a redox-inactive (or redox-silent) material is insulating.…”

Section: Introductionmentioning

confidence: 99%

“…Bovine serum albumin (BSA) is an important protein which is popularly used to block non-specific sites in the immunosensing platforms [9][10][11][12][13][14][15][16][17]. In electrochemical immunosensors, the sensing mechanism is generally dominated by capacitance (i.e., non-Faradaic detection) due to the redox-inactivity of the electrode platform upon which the antibody or antigen is immobilized.…”

Section: Introductionmentioning

confidence: 99%

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Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

et al. 2021

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Graphical Abstract This work investigates how bovine serum albumin (BSA), a commonly used protein in the fabrication of electrochemical immunosensors, can impact on the sensitivity of detection when integrated with antibody (Ab) pre-encapsulated with (i) insulating polyacrylonitrile (PAN) fibre (i.e., GCE-PAN-Ab-BSA immunosensor) or (ii) conducting PAN-grafted iron (II) phthalocyanine (FePc) (i.e., GCE-PAN@FePc-Ab-BSA immunosensor), using Vibrio cholerae toxin as a case study bioanalyte. Both immunosensors show different charge-transfer kinetics that strongly impact on their immunosensitive detection. From the electrochemical data, GCE-PAN-Ab-BSA is more insulating with the presence of BSA, while the GCE-PAN@FePc-Ab-BSA is more conducting with BSA. The CV of the GCE-PAN-Ab-BSA is dominated by radial diffusion process, while that of the GCE-PAN@FePc-Ab-BSA is planar diffusion process. The behaviour of GCE-PAN@FePc-Ab-BSA has been associated with the facile coordination of BSA and FePc that permits co-operative charge-transport of the redox probe, while that of the GCE-PAN-Ab-BSA is related to the interaction-induced PAN-BSA insulating state that suppresses charge-transport. As a consequence of these different interaction processes, GCE-PAN-Ab-BSA immunosensor provides higher electroanalytical performance for the detection of Vibrio cholerae toxin (with sensitivity of 16.12 Ω/log [VCT, g/mL] and limit of detection (LoD) of 3.20 × 10 −13 g/mL compared to those of the GCE-PAN@FePc-Ab-BSA (4.16 Ω/log (VCT, g mL −1 ) and 2.00 × 10 −12 g/mL). The study confirms the need for a thorough understanding of the physico-chemistries of the electrode platforms for the construction of immunosensors. Although this work is on immunosensors for cholera infection, it may well apply to other immunosensors.

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Detection of foodborne pathogens in contaminated food using nanomaterial-based electrochemical biosensors

Flores-Ramírez,

González-Estrada,

Chacón-López

et al. 2024

Analytical Biochemistry

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Onion-like carbon re-inforced electrospun polyacrylonitrile fibres for ultrasensitive electrochemical immunosensing of Vibrio cholerae toxin

Cited by 9 publications

References 34 publications

Ionic Liquid and f‐MWCNTs Fabricated Glassy Carbon Electrode for Determination of Amygdalin in Apple Seeds

Ionic Liquid and f‐MWCNTs Fabricated Glassy Carbon Electrode for Determination of Amygdalin in Apple Seeds

Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

Detection of foodborne pathogens in contaminated food using nanomaterial-based electrochemical biosensors

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