One-step separation of β-galactosidase from β-lactoglobulin using water-in-oil microemulsions

Mazı, Bekir Gökçen; Hamamcı, Haluk; Dungan, Stephanie R.

doi:10.1016/j.foodchem.2011.10.085

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…It is widely used in many elds including extraction and separation of various species. 1 The water core in microemulsion facilitates effective extraction of proteins [2][3][4][5][6] and it helps to maintain the activity of proteins during the extraction process. 7 The use of organic solvents in conventional microemulsion systems causes serious adverse effects on protein structure and property, which nd limited application in protein separation.…”

Section: Introductionmentioning

confidence: 99%

A dual-ionic liquid microemulsion system for the selective isolation of hemoglobin

et al. 2014

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A novel dual-ionic liquid (IL) microemulsion system is developed with IL 1-decyl-3-methylimidazolium bromide (DmimBr) as the surfactant and IL 1-butyl-3-methylimidazolium hexaflourophosphate (BmimPF 6 ) as a substitute for organic solvent. The phase diagram of this dual-IL microemulsion system clearly shows the formation of a single phase within a wide range of BmimPF 6 (1.76-98.3%, wt) and in the presence of an appropriate amount of DmimBr and water. The microemulsion is characterized by means of FT-IR spectra, dynamic light scattering and molecular probe. The dual-IL microemulsion system has been demonstrated to be effective for the extraction of proteins, and exhibited an obvious improvement in extraction efficiency for hemoglobin in comparison with pure BmimPF 6 . When 100 mL microemulsion is used to extract 100 mg mL À1 of proteins in an equal volume of sample solution, high selective extraction of hemoglobin (Hb) has been observed at pH 5. This might be attributed to the coordination interaction between the heme group of Hb and the imidazolium cationic moiety in the ionic liquids. Hb transferred into the microemulsion can be readily recovered by back extraction with Britton-Robinson buffer at pH 12, giving rise to a recovery of 55.6%. The dual-IL microemulsion system is practically applied to the isolation of Hb from human whole blood and the SDS-PAGE indicates that hemoglobin has been selectively isolated from human blood in the presence of co-existing protein species.

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Section: Introductionmentioning

confidence: 99%

A dual-ionic liquid microemulsion system for the selective isolation of hemoglobin

et al. 2014

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“… 11 also showed that low concentration of rhamnolipids biosurfactant (2.15 g/L) was optimum for the extraction of laccase. Other studies using chemical surfactants needed higher amount of surfactants in order to achieve significant extraction of protein, such as 11.11 g/L AOT for lipase extraction 12 , 14.58 g/L AOT for bovine serum albumin extraction 32 , 18.22 g/L for nattokinase extraction 13 , 88.91 g/L for β-galactosidase extraction 33 , or 7.29 g/L CTAB for tannase extraction 27 . By using biosurfactants to replace chemical surfactants in reverse micelle extraction, the amount of surfactants can be greatly reduced.…”

Section: Resultsmentioning

confidence: 99%

Reverse micelle Extraction of Antibiotics using an Eco-friendly Sophorolipids Biosurfactant

Chuo

Abd-Talib

Mohd‐Setapar

et al. 2018

Sci Rep

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Reverse micelles extraction of erythromycin and amoxicillin were carried out using the novel Sophorolipids biosurfactant. By replacing commonly used chemical surfactants with biosurfactant, reverse micelle extraction can be further improved in terms of environmental friendliness and sustainability. A central composite experimental design was used to investigate the effects of solution pH, KCl concentration, and sophorolipids concentration on the reverse micelle extraction of antibiotics. The most significant factor identified during the reverse micelle extraction of both antibiotics is the pH of aqueous solutions. Best forward extraction performance for erythromycin was found at feed phase pH of approximately 8.0 with low KCl and sophorolipids concentrations. Optimum recovery of erythromycin was obtained at stripping phase pH around 10.0 and with low KCl concentration. On the other hand, best forward extraction performance for amoxicillin was found at feed phase pH around 3.5 with low KCl concentration and high sophorolipids concentration. Optimum recovery of erythromycin was obtained at stripping phase pH around 6.0 with low KCl concentration. Both erythromycin and amoxicillin were found to be very sensitive toaqueous phase pH and can be easily degraded outside of their stable pH ranges.

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“…). Therefore numerous studies have been carried out in protein, amino acid and DNA extractions with reversed micellar system . Sodium (bis‐2‐ethylhexyl) sulfosuccinate, an anionic surfactant, was widely employed to form reverse micelles.…”

Section: Introductionmentioning

confidence: 99%

Purification of α‐glucosidase from mouse intestine by countercurrent chromatography coupled with a reverse micelle solvent system

Zou

et al. 2016

J of Separation Science

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Countercurrent chromatography coupled with a reverse micelle solvent was applied to separate α-glucosidase, which is stable at pH 6.0-8.8, 15-50°C. The separation conditions are as follows: stationary phase: pH 4.0 Tris-HCl buffer phase containing 50 mM Tris-HCl and 50 mM KCl; mobile phase A: isooctane containing 50 mM anionic surfactant sodium di(2-ethylhexyl)sulfosuccinate; mobile phase B: 50 mM Tris-HCl buffer containing 500 mM KCl (pH 8.0); In total, 25 mL (23.9 mg) crude enzyme was injected through the injection valve, the enzymatic reaction and sodium dodecylsulfate polyacrylamide gel electrophoresis results imply that the activity of purified α-glucosidase is 6.63-fold higher than that of the crude enzyme. Therefore, countercurrent chromatography coupled with a reverse micelle solvent is capable for protein separation and enrichment.

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One-step separation of β-galactosidase from β-lactoglobulin using water-in-oil microemulsions

Cited by 6 publications

References 34 publications

A dual-ionic liquid microemulsion system for the selective isolation of hemoglobin

A dual-ionic liquid microemulsion system for the selective isolation of hemoglobin

Reverse micelle Extraction of Antibiotics using an Eco-friendly Sophorolipids Biosurfactant

Purification of α‐glucosidase from mouse intestine by countercurrent chromatography coupled with a reverse micelle solvent system

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