Purification of α‐glucosidase from mouse intestine by countercurrent chromatography coupled with a reverse micelle solvent system

He, Kai; Zou, Zongyao; Hu, Yinran; Yang, Yong; Xiao, Yali; Gao, Pincao; Li, Xuegang; Yang, Xiaoli

doi:10.1002/jssc.201501092

Cited by 10 publications

(6 citation statements)

References 23 publications

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“…The residues were dissolved in methanol, and the sample solution was filtered through a 0.22-μm membrane filter before it was injected into the UPLC system. The fractions with purity <90% were further purified by preparative HPLC [30,31].…”

Section: Methodsmentioning

confidence: 99%

HSCCC Separation of the Two Iridoid Glycosides and Three Phenolic Compounds from Veronica ciliata and Their in Vitro Antioxidant and Anti-Hepatocarcinoma Activities

Sun

Shu

et al. 2016

Molecules

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Five main compounds, including two iridoid glycosides (catalposide, verproside) and three phenolic compounds (luteolin, 4-hydroxy benzoic acid, 3,4-dihydroxy benzoic acid), were separated and prepared from the crude extract of Veronica ciliata by high-speed countercurrent chromatography. n-Hexane/n-butanol/water (1.5:5:5, v/v/v) was used for the separation of catalposide and verproside. n-Hexane/n-butanol/water (3:2:5, v/v/v) was used for the separation of luteolin, 4-hydroxy benzoic acid and 3,4-dihydroxy benzoic acid. The head-to-tail elution mode was used with a flow rate of 5.0 mL/min and a rotary speed of 800 rpm. Finally, a total of 1.28 mg luteolin, 6 mg 4-hydroxy benzoic acid, 2 mg 3,4-dihydroxy benzoic acid, 2 mg verproside and 10 mg catalposide with purities of 98%, 99.1%, 99.5%, 99.8% and 99%, respectively, were obtained from 200 mg of crude extract. In addition, their structure was identified using MS, 1 H-NMR and 13 C-NMR. To the best of our knowledge, this is the first report of the separation and purification of iridoid glycosides and phenolic compounds from V. ciliata by high-speed countercurrent chromatography (HSCCC). Among these compounds, luteolin, 4-hydroxy benzoic acid and 3,4-dihydroxy benzoic acid were separated from V. ciliata Fisch. for the first time. The results of the antioxidant activity show that protocatechuic acid and luteolin have strong antioxidant activity compared to 2,6-di-tert-butyl-4-methylphenol (BHT) and vitamin C (Vc). Five compounds also exhibited strong anti-hepatocarcinoma activities.

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Section: Methodsmentioning

confidence: 99%

HSCCC Separation of the Two Iridoid Glycosides and Three Phenolic Compounds from Veronica ciliata and Their in Vitro Antioxidant and Anti-Hepatocarcinoma Activities

Sun

Shu

et al. 2016

Molecules

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“…Inverse micelles are prepared using cationic, anionic, mixed, zwitterionic, amphiphilic, and nonionic surfactants [78]. Anionic surfactants, such as sulfosuccinic acid bis(2-ethylhexyl) ester sodium salt, are widely used as surfactants in the preparation of inverse micelles [79]. Cetyl-trimethyl-ammonium bromide (CTAB), chloroform, and butyl alcohol were used to prepare reverse micelles [80].…”

Section: Polymeric Inverse Micellementioning

confidence: 99%

A Review of Polymeric Micelles and Their Applications

2022

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Self-assembly of amphiphilic polymers with hydrophilic and hydrophobic units results in micelles (polymeric nanoparticles), where polymer concentrations are above critical micelle concentrations (CMCs). Recently, micelles with metal nanoparticles (MNPs) have been utilized in many bio-applications because of their excellent biocompatibility, pharmacokinetics, adhesion to biosurfaces, targetability, and longevity. The size of the micelles is in the range of 10 to 100 nm, and different shapes of micelles have been developed for applications. Micelles have been focused recently on bio-applications because of their unique properties, size, shape, and biocompatibility, which enhance drug loading and target release in a controlled manner. This review focused on how CMC has been calculated using various techniques. Further, micelle importance is explained briefly, different types and shapes of micelles are discussed, and further extensions for the application of micelles are addressed. In the summary and outlook, points that need focus in future research on micelles are discussed. This will help researchers in the development of micelles for different applications.

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“…More studies had shown that alkaline extraction of protein in tomato seeds indicates that the diffusion process of the protein inside the solid was the decisive step for extraction [8][9]. However, this extraction system was quite different from the reversed micelle extraction system [10]. Therefore, the kinetics of reversed micelle extraction of solid proteins needs further study.…”

Section: Introductionmentioning

confidence: 99%

Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

Wu¹,

Sun²,

Shi³

et al. 2018

Proceedings of the 2018 3rd International Conference on Modelling, Simulation and Applied Mathematics (MSAM 2018)

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In this paper, the kinetic process of extracting total protein from quinoa by AOT/ isooctane reverse micelles was systematically studied. The effects of extraction time, extraction temperature, particle size and oscillation rate on quinoa protein forward extraction rate were investigated respectively. Results showed that extraction of quinoa protein by AOT/ isooctane reverse micelles system was mainly controlled by internal diffusion, that is, the diffusion of protein from the interior of the particle to the surface was the control step of the reverse micelle extraction process. The extraction process can be explained by shrinking core model. The macroscopic dynamic equation of the protein forward extraction by the reverse micelles system was established and verified by extraction experiments at different temperatures. The experimental results indicated that the extraction process belonged to first order reaction and the apparent activation energy was 14.65kJ / mol. The kinetic equation model could better describe the reverse micelle extraction process of quinoa protein and could provide important theoretical reference for the comprehensive development and utilization of quinoa protein in the future.

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Purification of α‐glucosidase from mouse intestine by countercurrent chromatography coupled with a reverse micelle solvent system

Cited by 10 publications

References 23 publications

HSCCC Separation of the Two Iridoid Glycosides and Three Phenolic Compounds from Veronica ciliata and Their in Vitro Antioxidant and Anti-Hepatocarcinoma Activities

HSCCC Separation of the Two Iridoid Glycosides and Three Phenolic Compounds from Veronica ciliata and Their in Vitro Antioxidant and Anti-Hepatocarcinoma Activities

A Review of Polymeric Micelles and Their Applications

Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

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