2004
DOI: 10.1042/ba20030138
|View full text |Cite
|
Sign up to set email alerts
|

One‐step purification and kinetic properties of the recombinant l‐asparaginase from Erwinia carotovora

Abstract: ECAR-LANS, the recombinant L-asparaginase fromErwinia carotovora, is a prospective therapeutic enzyme for leukaemia treatment. An efficient and economical scheme was developed for the purification, cloning and expression in Eschericha coli of ECAR-LANS. More than 90 % purity, complemented with 72 % active enzyme recovery, was achieved with a single chromatographic purification step. The activity of purified L-asparaginase was 630 i.u./mg. The ECAR-LANS K m value was 98 × 10 −6 M for the main physiological subs… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
51
0
5

Year Published

2008
2008
2021
2021

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 72 publications
(57 citation statements)
references
References 29 publications
0
51
0
5
Order By: Relevance
“…There appears to be two minor components present in the AspMP preparation ( Figure 3). As previously reported (Krasotkina et al, 2004), these low-molecular-mass contaminants were probably proteolytic products of L-asparaginase II, which accumulated during storage of the enzyme solutions. Incidentally, no cocktail of protease inhibitors were employed in the purification process because their presence in a therapeutic protein must be avoided.…”
Section: Discussionmentioning
confidence: 63%
See 4 more Smart Citations
“…There appears to be two minor components present in the AspMP preparation ( Figure 3). As previously reported (Krasotkina et al, 2004), these low-molecular-mass contaminants were probably proteolytic products of L-asparaginase II, which accumulated during storage of the enzyme solutions. Incidentally, no cocktail of protease inhibitors were employed in the purification process because their presence in a therapeutic protein must be avoided.…”
Section: Discussionmentioning
confidence: 63%
“…Previous reports cloned the DNA sequence of E. carotovora L-asparaginase II containing the signal sequence (Krasotkina et al, 2004;Kotzia and Labrou, 2005). Our aim was to compare expression and activity of two constructs of E. carotovora L-asparaginase II (with and without signal peptide) to optimize the process for large scale production.…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations