One-step method embedding superoxide dismutase and gold nanoparticles in silica sol–gel network in the presence of cysteine for construction of third-generation biosensor

Di, Junwei; Peng, Shaohua; Shen, Chunping; Yan-sheng, Gao; Tu, Yifeng

doi:10.1016/j.bios.2007.03.011

Cited by 56 publications

(20 citation statements)

References 32 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A peak-like response was observed because the concentration of superoxide anion decreased rapidly to the base level due to the dismutation in aqueous solution (Fig. 6), which is agreement with our previous reports [16,21,25].…”

Section: Optimization Of the Enzyme Electrode Preparationsupporting

confidence: 81%

“…It can be seen that a maximum response occurs at concentration of SOD at 250 U mL -1 in the mixed sol solution. This is consistent with our previous report [16,21]. Therefore, 250 U mL -1 SOD in the mixed solution was used for subsequent experiments.…”

Section: Optimization Of the Enzyme Electrode Preparationsupporting

confidence: 76%

“…Gold nanoparticles (GNPs) as a base for the immobilization of SOD have also been used to realize direct electron transfer of SOD duo to their excellent biological compatibility and large surface area [18][19][20]. With the benefits of the advantage of GNPs, self-assembly and the microenvironment of SG network structure, we also developed a high sensitive and long-time stable superoxide anion biosensor based on embedding SOD and GNPs in silica sol-gel (SG) network in the presence of cysteine [21].…”

Section: Superoxide Anion (Omentioning

confidence: 98%

“…Superoxide anion was generated by addition of sodium hydroxide to dimethylsulphoxide (DMSO) [21,24]. Briefly, the DMSO solution contained 5 mmol L -1 NaOH and 1% (v/v) H 2 O was bubbled by air for about 3 min and then stabilized about 30 min.…”

Section: Optimization Of the Enzyme Electrode Preparationmentioning

confidence: 99%

See 3 more Smart Citations

Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol–gel matrix at gold nanoparticles modified ITO electrode

Wang¹,

Wu²,

Wang³

et al. 2008

Bioprocess Biosyst Eng

Self Cite

View full text Add to dashboard Cite

A novel disposable biosensor based on direct electron transfer of superoxide dismutase (SOD) was fabricated for the determination of superoxide anion. The biosensor was constructed by electrodeposition of gold nanoparticles (GNPs) on the indium tin oxide (ITO) electrode and then immobilization of SOD in silica sol-gel (SG) network in the presence of cysteine on GNPs/ITO modified electrode surface. The distribution of GNPs on ITO electrode surface was examined by scanning electron microscopy (SEM). The immobilized SOD exhibited high catalytical activity towards superoxide anion. Parameters affecting the performance of the biosensor were also investigated. A linear calibration curve was obtained over the range from 0.08 to 0.64 microM with a correlation coefficient of 0.9937. The resulted biosensors were demonstrated to possess striking analytical properties for superoxide anion determination, such as high sensitivity, good accuracy, and long-term stability. It provides a promising platform for the fabrication of disposable biosensors.

show abstract

Section: Optimization Of the Enzyme Electrode Preparationsupporting

confidence: 81%

Section: Optimization Of the Enzyme Electrode Preparationsupporting

confidence: 76%

Section: Superoxide Anion (Omentioning

confidence: 98%

Section: Optimization Of the Enzyme Electrode Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol–gel matrix at gold nanoparticles modified ITO electrode

Wang¹,

Wu²,

Wang³

et al. 2008

Bioprocess Biosyst Eng

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several additives can be added to the sol-gel in different phases of the preparation, resulting in "tailored materials" suitable for a number of proteins and detection systems [50]. Table 1 summarizes some applications of silica gel-encapsulated proteins reported in the literature [183,194,[200][201][202][203][204][205][206][207][208][209][210][211][212][213][214][215][216][217]. Notable examples include cases in which more proteins are co-encapsulated to immobilize short metabolic pathways [194].…”

Section: Proteins Encapsulated In Silica Gel As Biosensorsmentioning

confidence: 99%

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

Ronda

Bruno

Campanini

et al. 2015

COC

View full text Add to dashboard Cite

Abstract:The development of silica-based sol-gel techniques compatible with the retention of protein structure and function started more than 20 years ago, mainly for the design of biotechnological devices or biomedical applications. Silica gels are optically transparent, exhibit good mechanical stability, are manufactured with different geometries, and are easily separated from the reaction media. Biomolecules encapsulated in silica gel normally retain their structural and functional properties, are stabilized with respect to chemical and physical insults, and can sometimes exhibit enhanced activity in comparison to the soluble form. This review briefly describes the chemistry of protein encapsulation within the pores of a silica gel three-dimensional network, the mechanism of interaction between the protein and the gel matrix, and its effects on protein structure, function, stability and dynamics. The main applications in the field of biosensor design are described. Special emphasis is devoted to silica gel encapsulation as a tool to selectively stabilize subsets of protein conformations for biochemical and biophysical studies, an application where silica-based encapsulation demonstrated superior performance with respect to other immobilization techniques.

show abstract

Electrocatalytic Behavior and Amperometric Detection of Morphine on ITO Electrode Modified with Directly Electrodeposited Gold Nanoparticles

Zhao¹,

Wu²,

Zhang³

et al. 2009

Electroanalysis

Self Cite

View full text Add to dashboard Cite

A gold nanoparticles (AuNPs) modified indium tin oxide (ITO) film coated glass electrode was prepared via a novel electrochemical deposition technique. The UV-visible spectrum and SEM indicated that the AuNPs on ITO electrode surface were spherical shape and quite symmetric distributed. The modified electrode exhibited excellent catalytic activity for the oxidation of morphine (MO). At optimal experimental condition, the oxidation current was responsive with the MO concentrations ranging from 8.0 Â 10 À7 to 1.6 Â 10 À5 M, the detection limit was 2.1 Â 10 -7 M. The modified electrode also exhibited high stability and reproducibility. The average recoveries of detection MO in human urine were ranged between 91.95% and 92.23%, and the RSD was less than 3.68% (n ¼ 5).

show abstract

One-step method embedding superoxide dismutase and gold nanoparticles in silica sol–gel network in the presence of cysteine for construction of third-generation biosensor

Cited by 56 publications

References 32 publications

Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol–gel matrix at gold nanoparticles modified ITO electrode

Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol–gel matrix at gold nanoparticles modified ITO electrode

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

Electrocatalytic Behavior and Amperometric Detection of Morphine on ITO Electrode Modified with Directly Electrodeposited Gold Nanoparticles

Contact Info

Product

Resources

About