One-step lipid extraction for plasma lipidomics analysis by liquid chromatography mass spectrometry

Satomi, Yoshinori; Hirayama, Megumi; Kobayashi, Hiroyuki

doi:10.1016/j.jchromb.2017.08.020

Cited by 59 publications

(47 citation statements)

References 37 publications

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“…At present, the most commonly used pretreatments and detection technologies include SPE, LLE , LC–MS, and GC–MS , etc. However, conventional extraction methods like hydrophilic polymer‐based SPE and LLE have several shortcomings, such as long sample preparation time , serious loss of analyte, a limited selection of extractants suitable for LLE, and a narrow pH range . SPE technology has become an ideal choice due to several advantages, which include rapid enrichment and separation, high recovery, good precision, and less sample consumption.…”

Section: Introductionmentioning

confidence: 99%

Preparation of restricted access media molecularly imprinted polymers for efficient separation and enrichment ofloxacin in bovine serum samples

Zhao

Chanling

et al. 2019

J of Separation Science

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We prepared ofloxacin restricted access media molecularly imprinted polymers using surface-initiated atom transfer radical polymerization on the surface of brominated silica gel using ofloxacin as a template molecule, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a crosslinking agent. We then characterized and studied the surface morphology and adsorption properties of the polymer. Experimental results show that saturation is reached within 25 min, and that the saturated adsorption capacity was 80.67 mg/g and the imprinting factor was 1.94. Our findings also showed that the polymer surface had good hydrophilicity and an excellent protein exclusion rate, which was 98.49%. The restricted access media molecularly imprinted polymers were then successfully applied to the enrichment and separation of ofloxacin in bovine serum. When combined with high-performance liquid chromatography, and the average recovery of ofloxacin was 95.6%, and the relative standard deviation was in the range of 2.47-3.38%. In a word, the restricted access media molecularly imprinted polymers is a method that involves a simple preparation procedure that results in excellent performance, which is a great improvement in the speed of detection of antibiotics. These qualities are what bestow upon this method its great potential for broad application. K E Y W O R D Shigh performance liquid chromatography, molecularly imprinted polymers, ofloxacin, restricted access media, solid phase extraction

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Section: Introductionmentioning

confidence: 99%

Preparation of restricted access media molecularly imprinted polymers for efficient separation and enrichment ofloxacin in bovine serum samples

Zhao

Chanling

et al. 2019

J of Separation Science

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“…Another report showed that recovery values for PC, PE and PI species were almost equivalent for methanol, ethanol and 2-propanol. Slightly higher recoveries of some LPC species were observed for methanol, which also proved to be the best for all tested LPI species [83].…”

Section: Isolation Of Pl Fractionsmentioning

confidence: 89%

Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis

Parchem

Sasson

Ferreri

et al. 2019

Free Radical Research

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Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterised by high nutritional value and other beneficial health effects, which are confirmed by numerous epidemiological studies. For this reason, PLs are of high interest in lipidomics that targets both the analysis of membrane lipid distribution as well as correlates composition of lipids with their effects on functioning of cells, tissues and organs. Lipidomic assessments follow-up the changes occurring in living organisms, such as free radical attack and oxidative modifications of the polyunsaturated fatty acids (PUFAs) build in PL structures. Oxidised PLs (oxPLs) can be generated exogenously and supplied to organisms with processed food or formed endogenously as a result of oxidative stress. Cellular and tissue oxPLs can be a biomarker predictive of the development of numerous diseases such as atherosclerosis or neuroinflammation. Therefore, suitable high-throughput analytical techniques, which enable comprehensive analysis of PL molecules in terms of the structure of hydrophilic group, fatty acid (FA) composition and oxidative modifications of FAs, have been currently developed. This review addresses all aspects of PL analysis, including lipid isolation, chromatographic separation of PL classes and species, as well as their detection. The bioinformatic tools that enable handling of a large amount of data generated during lipidomic analysis are also discussed. In addition, imaging techniques such as confocal microscopy and mass spectrometry imaging for analysis of cellular lipid maps, including membrane PLs, are presented.

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“…A recently published comparison of chloroform and MTBE protocols suggests that the MTBE method is more efficient for glycerophospholipids, ceramides, and unsaturated fatty acids, while the chloroform protocol is superior for saturated fatty acids and plasmalogens [14]. Recently, an interesting one-step extraction protocol for mouse plasma was published by Satomi et al [15]. The authors compare methanol, ethanol, 2-propanol, and acetonitrile to the MTBE method described above.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

“…To deal with such uncertainty, many users have transferred from triple-quadrupole instruments to high-resolution mass spectrometers with quadrupole-time of flight (Q-TOF) or Orbitrap technology [27,29,31]. While Q-TOF-based technology offers the mass resolution of 40,000 needed to separate the above-described isobaric example of plasmalogens and diacyl PC species, the mass resolution required to separate 13 C, 17 O, 2 H, or 15 N isotopologues from either each other or other monoisotopic lipid species is above 100,000, and can only be provided by Orbitrap or ion cyclotron technology. Nevertheless, in natural samples, all minor isotopes except for 13 C can be neglected for practical purposes due to their low abundances, but it is highly beneficial to separate the M + 2 isotopic peak of a lipid, which is mainly due to 13 C, from the monoisotopic mass peak of the same lipid with one double bond less.…”

Section: Direct Infusion Lipidomicsmentioning

confidence: 99%

“…For more information on the correlation between mass resolution and identification ambiguities, readers are referred to Bielow et al, who cover this topic in depth [32]. Instrumentation with a mass resolution beyond 500,000 makes it possible to perform isotopic labeling experiments and subsequent metabolic flux analysis with isotopes that have naturally low abundances, such as the technique proposed by Schuhmann et al for 15 N labeling in human HepG2 cells [33]. A recently published and interesting approach to addressing the ion suppression challenges arising from direct infusion is spectral stitching [33,34].…”

Section: Direct Infusion Lipidomicsmentioning

confidence: 99%

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Lipidomics from sample preparation to data analysis: a primer

2019

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Lipids are amongst the most important organic compounds in living organisms, where they serve as building blocks for cellular membranes as well as energy storage and signaling molecules. Lipidomics is the science of the large-scale determination of individual lipid species, and the underlying analytical technology that is used to identify and quantify the lipidome is generally mass spectrometry (MS). This review article provides an overview of the crucial steps in MS-based lipidomics workflows, including sample preparation, either liquid-liquid or solid-phase extraction, derivatization, chromatography, ion-mobility spectrometry, MS, and data processing by various software packages. The associated concepts are discussed from a technical perspective as well as in terms of their application. Furthermore, this article sheds light on recent advances in the technology used in this field and its current limitations. Particular emphasis is placed on data quality assurance and adequate data reporting; some of the most common pitfalls in lipidomics are discussed, along with how to circumvent them.

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One-step lipid extraction for plasma lipidomics analysis by liquid chromatography mass spectrometry

Cited by 59 publications

References 37 publications

Preparation of restricted access media molecularly imprinted polymers for efficient separation and enrichment ofloxacin in bovine serum samples

Preparation of restricted access media molecularly imprinted polymers for efficient separation and enrichment ofloxacin in bovine serum samples

Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis

Lipidomics from sample preparation to data analysis: a primer

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