Preparation of restricted access media molecularly imprinted polymers for efficient separation and enrichment ofloxacin in bovine serum samples

Abstract

We prepared ofloxacin restricted access media molecularly imprinted polymers using surface-initiated atom transfer radical polymerization on the surface of brominated silica gel using ofloxacin as a template molecule, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a crosslinking agent. We then characterized and studied the surface morphology and adsorption properties of the polymer. Experimental results show that saturation is reached within 25 min, and that the saturated adsorption capacity was 80.67 mg/g and the imprinting factor was 1.94. Our findings also showed that the polymer surface had good hydrophilicity and an excellent protein exclusion rate, which was 98.49%. The restricted access media molecularly imprinted polymers were then successfully applied to the enrichment and separation of ofloxacin in bovine serum. When combined with high-performance liquid chromatography, and the average recovery of ofloxacin was 95.6%, and the relative standard deviation was in the range of 2.47-3.38%. In a word, the restricted access media molecularly imprinted polymers is a method that involves a simple preparation procedure that results in excellent performance, which is a great improvement in the speed of detection of antibiotics. These qualities are what bestow upon this method its great potential for broad application. K E Y W O R D Shigh performance liquid chromatography, molecularly imprinted polymers, ofloxacin, restricted access media, solid phase extraction

Recent developments on magnetic molecular imprinted polymers (MMIPs) for sensing, capturing, and monitoring pharmaceutical and agricultural pollutants

“…A high selectivity factor (SF) of >11.1 was recorded for all three competitors whereas the nonimprinted MNIP showed an SF around 2. These figures are comparable with the nonmagnetic MIP for other pharmaceutical pollutants reported in the literature 75,76 . The same technology should be applicable for the preparation of MMIP particles for many other organic pollutants, suitable for their capture and monitoring.…”

Molecularly Imprinted Polymers in Biological Applications

“…Iridoid glycosides, spiramycin, 2,4-dichlorophenoxyacetic acid, diclofenac, alfatoxins [17][18][19][20][21] Suspension polymerization Malachite green, p-hydroxybenzoic acid, timolol, alfatoxins B1, Pb 2+ [22][23][24][25][26] Emulsion polymerization Quercetin, malachite green, Listeria monocytogenes, triazines, Cd 2+ [27][28][29][30][31] Seed polymerization Nicotinamide, bisphenol A, promazine derivates [32][33][34] Precipitation polymerization Triclosan and triclocarban, strychnine, caffeic acid, vitamin E, prednisolone [35][36][37][38][39] RAFT polymerization Benzimidazole, propranolol, polycyclic aromatic hydrocarbons [40][41][42] Atom transfer radical polymerization Ofloxacin, ␤2-agonists [43,44] Sol-gel Iprodione, methylxanthines [45,46] RAFT: Reversible addition fragmentation chain transfer.…”

A restricted access molecularly imprinted polymer coating on metal–organic frameworks for solid-phase extraction of ofloxacin and enrofloxacin from bovine serum

“…Recently, a RAMIP was prepared using 2 mm spherical silica gel and atom-transfer radical polymerization. 28 Although this material has some advantages in adsorption capacity and limits of detection, UiO-66-NH 2 @-RAMIP@BSA has a better adsorption rate, protein exclusion efficiency, and recovery.…”