One-step label-free chemiluminescent assay for determination of exonuclease III activity towards hairpin oligonucleotides

Peroxidase-Mimicking Activity of Nanoceria for Label-Free Colorimetric Assay for Exonuclease III Activity

“…However, despite their widespread use, both methods are time-consuming, labor intensive, and pose safety risks during the detection process [9,10]. Recently, alternative methods for detecting 3 -5 exonuclease activity have been developed, such as fluorescence-based methods utilizing the G-quadruplex [11][12][13][14][15] and FRET [16][17][18][19]. In addition, a detection method using metal nanoparticles, including copper nanoparticles [20,21], has been developed for detecting 3 -5 exonuclease activity.…”

Reusable and sensitive exonuclease III activity detection on DNB nanoarrays based on cPAS sequencing technology

“…Exonucleases are DNA enzymes that participate in a hydrolyzing reaction which break phosphodiester bonds at the end of a polynucleotide chain, and cleave one nucleotide at a time. The exonuclease family is concerned with the sustenance of cellular metabolism and physiological processes, such as aiding DNA proofreading and sustaining the stability of the genome [1]. Exonuclease III (Exo III) belongs to the exonuclease family with 3'-termini to 5' -termini exonuclease activities, that works by stepwise removal of mononucleotide from the 3'-OH termini of doublestranded DNA when the substrates are recessed or blunt, and the 3'-terminus does not require any specific recognition site and presents lower activities on single-stranded DNA or duplex DNAs with a protruding 3'-terminus, acts an indispensable role in some cellular and physiological processes, and is also imperative to preserving the stability of the genome [2].…”