One‐Step Formation of “Chain‐Armor”‐Stabilized DNA Nanostructures

Abstract

DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3'-ends and azides on their 5'-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.

Block Copolymer Micellization as a Protection Strategy for DNA Origami

“…[8][9][10][11][12] These can be decorated with many functional elements, [13,14] including (bio)molecules [15,16] or inorganic nanoparticles, [17][18][19][20] for potential applications in electronics, [21,22] photonics, [23] or biology and nanomedicine. [29][30][31] Exceptions include very small DNAstructures, [32] fully ligated motifs, [33,34] thin tubular structures, [30] and hollow wireframe structures, [35][36][37] which show an increased stability compared to common multilayer origami structures.A lternatively,alipid bilayer can protect DNAorigami, mimicking the structure of enveloped viruses. [29][30][31] Exceptions include very small DNAstructures, [32] fully ligated motifs, [33,34] thin tubular structures, [30] and hollow wireframe structures, [35][36][37] which show an increased stability compared to common multilayer origami structures.A lternatively,alipid bilayer can protect DNAorigami, mimicking the structure of enveloped viruses.…”

Picomolar Fingerprinting of Nucleic Acid Nanoparticles Using Solid-State Nanopores

“…24–47 Ongoing development of NANPs is motivated by their potential applications in medicine and biotechnology, including their uses as carriers for RNAi inducers, 47–50 aptamers, 48, 51 fluorophores, and as customizable materials. 52–54 Striking features of these nanoparticles are their precisely defined molecular structures, chemical stability, and tunable physiochemical properties. Moreover, due to their size uniformity and biocompatibility, NANPs are expected to outperform their metal and polymeric nanoparticle counterparts previously used for biosensing through antibody decoration, peptide coating, as well as DNA conjugation.…”

DNA nanostructures constructed with multi-stranded motifs

“…Similar fragility was also observed for other addressable structures from LEGO approach (22,26). It could be mitigated by structural enforcement treatments such as enzymatic and chemical ligation (26,29). …”