One-step double network hydrogels of photocurable monomers and bacterial cellulose fibers

Roig-Sánchez, Soledad; Kam, Doron; Malandain, Nanthilde; Keneth, Ela Sachyani; Shoseyov, Oded; Magdassi, Shlomo; Laromaine, Anna; Roig, Anna

doi:10.1016/j.carbpol.2022.119778

Cited by 13 publications

(9 citation statements)

References 52 publications

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“…Then, the former bacterial solution in a proportion of 1:14 with HS fresh medium was poured into 12 × 12 cm 2 plates and cultured for 7 days at 30 °C. As-obtained BC films were soaked in a As previously reported by Roig-Sanchez et al, 46 bacterial nanocellulose fibers (BCf) were obtained after blending five films of 144 cm 2 in 1 L of Milli-Q water with a commercial household blender (Jata electro Mod. BT1200, maximum speed, 10 min).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain,

Sanz-Fraile,

Farré

et al. 2023

ACS Appl. Bio Mater.

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There is a growing interest in developing natural hydrogel-based scaffolds to culture cells in a three-dimensional (3D) millieu that better mimics the in vivo cells' microenvironment. A promising approach is to use hydrogels from animal tissues, such as decellularized extracellular matrices; however, they usually exhibit suboptimal mechanical properties compared to native tissue and their composition with hundreds of different protein complicates to elucidate which stimulus triggers cell's responses. As simpler scaffolds, type I collagen hydrogels are used to study cell behavior in mechanobiology even though they are also softer than native tissues. In this work, type I collagen is mixed with bacterial nanocellulose fibers (BCf) to develop reinforced scaffolds with mechanical properties suitable for 3D cell culture. BCf were produced from blended pellicles biosynthesized from Komagataeibacter xylinus. Then, BCf were mixed with concentrated collagen from rat-tail tendons to form composite hydrogels. Confocal laser scanning microscopy and scanning electron microscopy images confirmed the homogeneous macro-and microdistribution of both natural polymers. Porosity analysis confirmed that BCf do not disrupt the scaffold structure. Tensile strength and rheology measurements demonstrated the reinforcement action of BCf (43% increased stiffness) compared to the collagen hydrogel while maintaining the same viscoelastic response. Additionally, this reinforcement of collagen hydrogels with BCf offers the possibility to mix cells before gelation and then proceed to the culture of the 3D cell scaffolds. We obtained scaffolds with human bone marrow-derived mesenchymal stromal cells or human fibroblasts within the composite hydrogels, allowing a homogeneous 3D viable culture for at least 7 days. A smaller surface shrinkage in the reinforced hydrogels compared to type I collagen hydrogels confirmed the strengthening of the composite hydrogels. These collagen hydrogels reinforced with BCf might emerge as a promising platform for 3D in vitro organ modeling, tissue-engineering applications, and suitable to conduct fundamental mechanobiology studies.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…However, the porosity range of pure BC films does not allow them to be used as scaffolds for 3D cell culture. Similarly, BC nanofibers (BCf) were used to reinforce photocurable hydrogels and natural polymers (collagen, , ECM, etc.) without greatly affecting their structure.…”

Section: Introductionmentioning

confidence: 99%

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain,

Sanz-Fraile,

Farré

et al. 2023

ACS Appl. Bio Mater.

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“…PC can full the requirement above as biomaterials for implants. PC is usually synthesized into different forms to reproduce as soft implant materials [67] or coating materials [68].…”

Section: Implant Materialsmentioning

confidence: 99%

Plant-based cellulose fiber as biomaterials for biomedical application: A Short Review

Azril

Jeng

Nugroho

2023

JFPC

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Cellulose is the most common polysaccharide that can be obtained from many resources. Plant-based cellulose (PC) is preferable due to its high renewability and availability in nature. This inherent affluence naturally opens the door to new applications for this adaptable material. PC provides various potential applications such as packaging, textile, and biomedical application. Currently, PC shows progress in its feasibility in biomedical applications because it fulfills the requirement of the characteristics of biomaterials such as biocompatible, biodegradable, anti-microbial, and enhancing tissue regeneration. Different morphological forms of PC such as fiber, microfibril/nanofibril cellulose (MFCs/NFCs), and micro/nanocrystalline cellulose (MCCs/NCCs) are adapted for different biomedical applications. This short review provides a general characteristic of plant-based cellulose (PC) and its potential to be applied in biomedical fields such as tissue engineering.

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“…Bacterial solutions were diluted with fresh media (1:14 ratio) and were maintained for 5−7 days in culture well plates, as previously described. 42 BC films were carefully harvested and washed once in 50% ethanol (Panreac), twice in boiling 0.1 M NaOH (Sigma-Aldrich), and then rinsed with water to obtain neutral pH. BC films were stored at room temperature in water until use.…”

Section: ■ Introductionmentioning

confidence: 99%

Conductive Bacterial Nanocellulose-Polypyrrole Patches Promote Cardiomyocyte Differentiation

Srinivasan

Cler

Zapata‐Arteaga

et al. 2023

ACS Appl. Bio Mater.

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The low endogenous regenerative capacity of the heart, added to the prevalence of cardiovascular diseases, triggered the advent of cardiac tissue engineering in the last decades. The myocardial niche plays a critical role in directing the function and fate of cardiomyocytes; therefore, engineering a biomimetic scaffold holds excellent promise. We produced an electroconductive cardiac patch of bacterial nanocellulose (BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the natural myocardial microenvironment. BC offers a 3D interconnected fiber structure with high flexibility, which is ideal for hosting Ppy nanoparticles. BC-Ppy composites were produced by decorating the network of BC fibers (65 ± 12 nm) with conductive Ppy nanoparticles (83 ± 8 nm). Ppy NPs effectively augment the conductivity, surface roughness, and thickness of BC composites despite reducing scaffolds’ transparency. BC-Ppy composites were flexible (up to 10 mM Ppy), maintained their intricate 3D extracellular matrix-like mesh structure in all Ppy concentrations tested, and displayed electrical conductivities in the range of native cardiac tissue. Furthermore, these materials exhibit tensile strength, surface roughness, and wettability values appropriate for their final use as cardiac patches. In vitro experiments with cardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibility of BC-Ppy composites. BC-Ppy scaffolds improved cell viability and attachment, promoting a desirable cardiomyoblast morphology. Biochemical analyses revealed that H9c2 cells showed different cardiomyocyte phenotypes and distinct levels of maturity depending on the amount of Ppy in the substrate used. Specifically, the employment of BC-Ppy composites drives partial H9c2 differentiation toward a cardiomyocyte-like phenotype. The scaffolds increase the expression of functional cardiac markers in H9c2 cells, indicative of a higher differentiation efficiency, which is not observed with plain BC. Our results highlight the remarkable potential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerative therapies.

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One-step double network hydrogels of photocurable monomers and bacterial cellulose fibers

Cited by 13 publications

References 52 publications

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Plant-based cellulose fiber as biomaterials for biomedical application: A Short Review

Conductive Bacterial Nanocellulose-Polypyrrole Patches Promote Cardiomyocyte Differentiation

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