One-Step Chemiluminescent Assay for Hydrogen Peroxide Analysis in Water

Abstract

The detection of hydrogen peroxide is of great importance in the environmental field. For this, a homogeneous technique has been developed here for sensitive and rapid quantification of hydrogen peroxide. In this technique, hemoglobin was used as a bioreceptor, where heme groups acted as electroactive centers to catalyze hydrogen peroxide reduction. The chemiluminescence reagent luminol is also a peroxidase substrate and can be oxidized by hemoglobin—thus generating a CL signal. The principle of the designed biosensor was based on the competition between hydrogen peroxide and luminol towards hemoglobin. Under optimized conditions, the chemiluminescent signal decreased with increasing hemoglobin concentrations within the linear range of 0.5 to 12 mM, with a correlation coefficient R2 of 0.99762. The limit of detection was calculated to be as low as 0.308 mM. The selectivity of the biosensor was successfully demonstrated against different interferents. The developed strategy provides a one step, simple, and low-cost bioanalytical method which can be applied for the monitoring of other peroxidase substrates.

Sensitive Detection of Trace Hydrogen Peroxide via Dual-Emissive Electrochemiluminescence from a Luminol/Porphyrin System: Comprehensive Innovative Experiments on Analytical Instruments for Undergraduates

“…The accurate detection of hydro-gen peroxide is of great significance for life and health. Common concentrations of H 2 O 2 range from 0.26 mg/kg in water to 0.37 mg/kg in tap water 3 and from 10 −9 to 10 −6 mol/ L in the normal human body. 4 At present, the main methods for detecting H 2 O 2 include flow injection conductivity, electroanalysis, 5 fluorescence analysis, 6 chemiluminescence, 7 and so on.…”

Antioxidant-rich brilliant polymeric nanocomposites for quick and efficient non-enzymatic hydrogen peroxide sensor

“…Hydrogen peroxide (H 2 O 2 ) plays a crucial role in various industries including biology, medicine, and food, making the quick and accurate determination of its presence essential. 7 Several analytical methods such as chemiluminescence, 8 spectroscopy, 9 and titrimetric analysis, have been utilized for this purpose. However, these methods are expensive, time-consuming, lack sensitivity and can be affected by other substances in the samples being analyzed.…”

One‐Pot Synthesis of Nanoflower‐Like Zn2SnS4 as Nanozymes for Highly Sensitive Electrochemical Detection of H2O2 Released by Living Cells

“…[2,3] The development of efficient and reliable modus for the sensitive and selective detection of H 2 O 2 under physiological conditions is of paramount importance. In the past two decades, a variety of methods have been utilized to H 2 O 2 detection, including colorimetry, [4] spectrometry, [5] chemiluminescence, [6] positron emission tomography, [7] electrochemical methods, [8] and more comparison of the analytical performance of other assay methods on H 2 O 2 detection have been exhibited in Table S-1. The electrochemical method has garnered significant attention due to its inherent simplicity, rapidity, ease of handling, and cost-effectiveness.…”