One single HPLC-PDA/(-)ESI-MS/MS analysis to simultaneously determine 30 components of the aqueous extract of Rabdosia rubescens

Tang, Jun‐Ming; Zhao, Ming; Wang, Yuji; Kang, Guifeng; Wu, Jianhui; Zheng, Meiqing; Peng, Shiqi

doi:10.1016/j.jchromb.2011.07.046

Cited by 12 publications

(9 citation statements)

References 24 publications

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“…In total, the content of eleven components reached to 62.45% of the total amount, and oridonin was found as the main component. These compounds had been reported by previous study that determined thirty components of the aqueous extract of R. rubescens (Tang et al ., ). In present work, only thirteen components have been isolated from R. rubescens , which was different from the previous results.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, some products like R. rubescens tea, tablets and the essential oil of R. rubescens have obtained more and more attentions of the consumers in China. The R. rubescens contained abundant bioactive compounds such as diterpenoids, flavonoids, phenolic compounds and so on (Liu et al ., ; Tang et al ., ). These compounds showed various biological activities including anti‐inflammatory, antitumor actions, antibacterial and antioxidant activities (Hsieh et al ., ; Bai et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Profile of antioxidant and antibacterial activities of different solvent extracts from Rabdosia rubescens

Feng

2014

Int J of Food Sci Tech

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Summary The objective of this work was to investigate the antioxidant and antibacterial activities of different extracts from Rabdosia rubescens and to further evaluate the antibacterial mechanism of extracts. The results showed that 80% acetone extracts had the highest contents of total polyphenols (8.09 mg GAE g−1) and flavonoids (5.69 mg RE g−1) and exhibited the strongest antioxidant activities, followed by 80% methanol and 80% ethanol, and the lowest for hexane extracts. Others except for hexane extracts showed different antibacterial activities against Gram‐positive strains, while no inhibitory effects were found on tested Gram‐negative bacterial strains. Among these extracts, 80% acetone and ethanol extracts had relatively higher antibacterial activities with the lowest minimum inhibitory concentration and minimum bactericidal concentration values of 5 and 10 mg mL−1. The antibacterial mechanism of ethanol extracts against Staphylococcus aureus might be described as it disrupts cell wall, increases cell membrane permeability and then leads to the leakage of cell constituents.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Profile of antioxidant and antibacterial activities of different solvent extracts from Rabdosia rubescens

Feng

2014

Int J of Food Sci Tech

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“…Besides, this aqueous extract gave the same chromatogram ( Figure 5B) of HPLC-PDA previously reported. 15 The yield, solubility, and composition of the extract demonstrate that this procedure is suitable for preparing AERL. The IC 50 values in Figure 1 not only indicate AERL can effectively inhibit platelet aggregation in vitro, but also show that the potency of AERL inhibiting platelet aggregation induced by TH was twofold, tenfold, and 12-fold higher than that of AERL inhibiting platelet aggregation induced by AA, ADP, and PAF, respectively, and that AERL therefore selectively inhibits TH-induced platelet aggregation.…”

Section: Discussionmentioning

confidence: 87%

“…The gradient elution program was described previously. 15 The flow rate of the mobile phase was 0.3 mL/min. After each run, the column was washed with acetonitrile and equilibrated to initial conditions for 15 minutes.…”

Section: Hplc-pda Analysismentioning

confidence: 99%

“…In brief, five nucleotides, 21 phenolic acids, and four diterpenoids of AERL, 15 of which the HPLC-PDA 3D chromatogram is shown in Figure 5 and Table S1, were docked into the active site of P-selectin. The docking demonstrated that rosmarinic acid had the lowest energy and could be assigned as the antithrombotic …”

Section: The Docking Feature Of Rosmarinic Acid In P-selectin's Activmentioning

confidence: 99%

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Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis

Wang¹,

Tang²,

Zhu³

et al. 2015

IJN

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The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory concentration values of aqueous extract of R. rubescens leaves (AERL) inhibiting platelet aggregation induced by thrombin, arachidonic acid, adenosine diphosphate, and platelet-activating factor ranged from 0.12 mg/mL to 1.43 mg/mL. The minimal effective oral dose of AERL inhibiting the rats from forming thrombus was 25 mg/kg. Both in vitro and in vivo actions were correlated with AERL concentration-dependently inhibiting sP-selectin release. In water, AERL formed nanoparticles, and their size depended on the concentration. Docking the five nucleotides, 21 phenolic acids, and four diterpenoids identified by high-performance liquid chromatography–photodiode array detector/(−)electrospray ionization-tandem mass spectrometry analysis into the active site of P-selectin, rosmarinic acid was predicted to be the antithrombotic ingredient of AERL. In flow cytometry analysis, 1 μM of rosmarinic acid effectively inhibited sP-selectin release in arachidonic acid-activated platelets. In a rat model, 5 mg/kg of oral rosmarinic acid effectively inhibited thrombosis.

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Optimisation of an Ultrasound‐assisted Extraction Followed by RP‐HPLC Separation for the Simultaneous Determination of Oleanolic Acid, Ursolic Acid and Oridonin Content in Rabdosia rubescens

Yang

Wei

Huang

2012

Phytochemical Analysis

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One single HPLC-PDA/(-)ESI-MS/MS analysis to simultaneously determine 30 components of the aqueous extract of Rabdosia rubescens

Cited by 12 publications

References 24 publications

Profile of antioxidant and antibacterial activities of different solvent extracts from Rabdosia rubescens

Profile of antioxidant and antibacterial activities of different solvent extracts from Rabdosia rubescens

Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis

Optimisation of an Ultrasound‐assisted Extraction Followed by RP‐HPLC Separation for the Simultaneous Determination of Oleanolic Acid, Ursolic Acid and Oridonin Content in Rabdosia rubescens

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