Optimisation of an Ultrasound‐assisted Extraction Followed by RP‐HPLC Separation for the Simultaneous Determination of Oleanolic Acid, Ursolic Acid and Oridonin Content in Rabdosia rubescens

Abstract

The combined UAE-HPLC process is a fast, convenient and appropriate method for the quantitative analysis of these three compounds in R. rubescens.

Ethanol solution-modified supercritical carbon dioxide extraction of triterpenic acids from Hedyotis corymbosa with ultrasound assistance and determination of their solubilities

“…The initial analysis conditions were chosen according to the literature [31]. The gradient elution protocol was optimized, and the final composition of mobile phase was described earlier in Section 2.4.…”

Kinetic and characterization studies for three bioactive compounds extracted from Rabdosia rubescens using ultrasound

“…Because OA, UA and oridonin are polar, a polar solvent, such as acetone, ethyl acetate, methanol or ethanol, should be used to effectively extract these analytes from R. rubescens (Yang et al, 2012). Of these solvents, ethanol is safe and provides both good yields and high concentrations for the three targeted compounds.…”

“…Many phytochemical studies focusing on this plant have found that it contains diterpenoids, flavonoids, phenolic acids, triterpenoids and volatile oils . Diterpenoids, such The target compounds are conventionally extracted from R. rubescens using solid-liquid extraction techniques, such as heat reflux and Soxhlet extraction with ethyl acetate (Jian et al, 2007), methanol Jin et al, 2010) and aqueous ethanol solutions (Yang et al, 2012). Many papers in literature discuss the obtaining of extract containing OA and UA from other natural materials using various organic solvents (Domingues et al, 2010;Yang et al, 2013b,c).…”

“…This herb is commonly used to treat various diseases, such as stomach aches, pharyngitis, sore throat, cough, wrestling injuries and tumors (Yang et al, 2012). This herb has also attracted considerable interest due to its antitumor activity.…”

Preparation and evaluation of molecularly imprinted composite membranes for inducing crystallization of oleanolic acid in supercritical CO2

“…1 Recently, more and more attentions are paid to OA, due to its pharmacological activities, such as anti-inflammation, [2][3][4] antivirus, 5 antitumor, [6][7][8][9] hepatoprotective, [9][10][11] hypolipidemic and hypoglycemic effects. Several conventional methods, [13][14][15][16][17][18] including solvent extraction, ultrasonic-assisted extraction, microwave-assisted extraction, ultra high pressure extraction, macroporous resin and silica gel column have been developed for the extraction and purification of OA. Several conventional methods, [13][14][15][16][17][18] including solvent extraction, ultrasonic-assisted extraction, microwave-assisted extraction, ultra high pressure extraction, macroporous resin and silica gel column have been developed for the extraction and purification of OA.…”