One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip

Abstract

Alpha-fetoprotein (AFP) is an important disease biomarker, related to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device is developed for rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only requires the operator add 40 μl sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic ranging from 10-1 000 ng/mL and LOD (1.88 ng/ml) within a short time of 3 min.Predictably, our method holds the great potential to be applied in POC diagnostics of proteins, especially for some regions with resource-limited.

A novel LoC-SERS device integrated with aptamer recognition strategy for the highly sensitive and specific detection of thrombin and platelet-derived growth factor-B

“…SERS microfluidic chip was formed by integrating SERS substrates into a microfluidic platform and is currently used for portable sensing techniques, such as detecting drugs, oligonucleotides, biomolecules, and proteins. [37][38][39] Moreover, LoC systems could serve as an integrated platform to make SERS detection more reproducible, efficient, safe, and environmentally friendly. 40 Most of the time, however, external pumps, connecting pipes, and other facilities are needed for microfluidic control.…”

Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

“…Hence, the development of power-free microfluidic chips to introduce samples and reagents is in high demand. In the past, various power-free microfluidic chips have been reported [ 1 , 2 , 3 , 4 ]. Nevertheless, the use of a microfluidic chip alone still involves multi-step operations to introduce a sample, mix the sample and several reagents for reactions, and detect the signals.…”