One-pot synthesis of functional peptide-modified gold nanoparticles for gene delivery

Xu, Xiaomin; Liu, Yang; Yang, Yujie; Wu, Jingjing; Cao, Meiwen; Sun, Li

doi:10.1016/j.colsurfa.2022.128491

Cited by 7 publications

(6 citation statements)

References 37 publications

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Section: Functionalization Of Au Npsmentioning

confidence: 99%

“…( A ) Different types of amino acid interactions with bacteria. ( B ) Nanoparticles allow easy functionalization of peptides for gene delivery, with several types of interactions between the peptide fragments being possible; stability increases [ 141 , 142 ].…”

Section: Functionalization Of Au Npsmentioning

confidence: 99%

“…These interactions can lead to free radical generation, which can cause membrane perturbations (pores in the membrane). Due to perturbations, there is oozing out of the bacterial cytoplasm, leading the cell to death due to loss of proteins, nucleic acids and cell structural rigidity [141,142]. Organic inhibitors, featuring hetero atoms, aromatic rings and conjugated systems, stand out as highly effective components in industrial applications [14,143,144].…”

Section: Peptides and Amino Acid Functionalized Au Npsmentioning

confidence: 99%

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Review of Advances in Coating and Functionalization of Gold Nanoparticles: From Theory to Biomedical Application

Arcos Rosero,

Bueno Barbezan,

Daruich de Souza

et al. 2024

Pharmaceutics

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Nanoparticles, especially gold nanoparticles (Au NPs) have gained increasing interest in biomedical applications. Used for disease prevention, diagnosis and therapies, its significant advantages in therapeutic efficacy and safety have been the main target of interest. Its application in immune system prevention, stability in physiological environments and cell membranes, low toxicity and optimal bioperformances are critical to the success of engineered nanomaterials. Its unique optical properties are great attractors. Recently, several physical and chemical methods for coating these NPs have been widely used. Biomolecules such as DNA, RNA, peptides, antibodies, proteins, carbohydrates and biopolymers, among others, have been widely used in coatings of Au NPs for various biomedical applications, thus increasing their biocompatibility while maintaining their biological functions. This review mainly presents a general and representative view of the different types of coatings and Au NP functionalization using various biomolecules, strategies and functionalization mechanisms.

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Section: Functionalization Of Au Npsmentioning

confidence: 99%

Section: Functionalization Of Au Npsmentioning

confidence: 99%

Section: Peptides and Amino Acid Functionalized Au Npsmentioning

confidence: 99%

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Review of Advances in Coating and Functionalization of Gold Nanoparticles: From Theory to Biomedical Application

Arcos Rosero,

Bueno Barbezan,

Daruich de Souza

et al. 2024

Pharmaceutics

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“…Peaks associated with amide I, II, and III absorption on FTIR spectra are noted as proof of peptide attachment to the AuNP surface. 53 Other surface and elemental analysis, such as atomic force microscopy 54 and Xray photoelectron spectroscopy, 55 have also been used to probe the surface environment on the AuNP. Mass spectrometry and amino acid analysis can provide an average estimate of the number of peptides per AuNP.…”

Section: Harnessing σ-Donating π-Bonding and Van Der Waalsmentioning

confidence: 99%

“…In addition to UV–vis, DLS, and ZP measurements, the physicochemical properties of the peptide-conjugated AuNPs are commonly characterized with Fourier transform infrared spectroscopy (FTIR). Peaks associated with amide I, II, and III absorption on FTIR spectra are noted as proof of peptide attachment to the AuNP surface . Other surface and elemental analysis, such as atomic force microscopy and X-ray photoelectron spectroscopy, have also been used to probe the surface environment on the AuNP.…”

Section: Categories Of Soft Surface Coatings and Ligandsmentioning

confidence: 99%

The Landscape of Gold Nanocrystal Surface Chemistry

et al. 2023

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Conspectus Gold nanoparticles (AuNPs) exhibit unique size- and shape-dependent properties not obtainable at the macroscale. Gold nanorods (AuNRs), with their morphology-dependent optical properties, ability to convert light to heat, and high surface-to-volume ratios, are of great interest for biosensing, medicine, and catalysis. While the gold core provides many fascinating properties, this Account focuses on AuNP soft surface coatings, which govern the interactions of nanoparticles with the local environments. Postmodification of AuNP surface chemistry can greatly alter NP colloidal stability, nano-bio interactions, and functionality. Polyelectrolyte coatings provide controllable surface-coating thickness and charge, which impact the composition of the acquired corona in biological settings. Covalent modification, in which covalently bound ligands replace the original capping layer, is often performed with thiols and disulfides due to their ability to replace native coatings. N-heterocyclic carbenes and looped peptides expand the possible functionalities of the ligand layer. The characterization of surface ligands bound to AuNPs, in terms of ligand density and dynamics, remains a challenge. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for understanding molecular structures and dynamics. Our recent NMR work on AuNPs demonstrated that NMR data were obtainable for ligands on NPs with diameters up to 25 nm for the first time. This was facilitated by the strong proton NMR signals of the trimethylammonium headgroup, which are present in a distinct regime from other ligand protons’ signals. Ligand density analyses showed that the smallest AuNPs (below 4 nm) had the largest ligand densities, yet spin–spin T2 measurements revealed that these smallest NPs also had the most mobile ligand headgroups. Molecular dynamics simulations were able to reconcile these seemingly contradictory results. While NMR spectroscopy provides ligand information averaged over many NPs, the ligand distribution on individual particles’ surfaces must also be probed to fully understand the surface coating. Taking advantage of improvements in electron energy loss spectroscopy (EELS) detectors employed with scanning transmission electron microscopy (STEM), a single-layer graphene substrate was used to calibrate the carbon K-edge EELS signal, allowing quantitative imaging of the carbon atom densities on AuNRs with sub-nanometer spatial resolution. In collaboration with others, we revealed that the mean value for surfactant-bilayer-coated AuNRs had 10–30% reduced ligand density at the ends of the rods compared to the sides, confirming prior indirect evidence for spatially distinct ligand densities. Recent work has found that surface ligands on nanoparticles can, somewhat surprisingly, enhance the selectivity and efficiency of the electrocatalytic reduction of CO2 by controlling access to the active site, tuning its electronic and chemical environment, or denying entry to impurities that poison the nanoparticle surface to facilitate ...

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