One-pot process for lactic acid production from wheat straw by an adapted Bacillus coagulans and identification of genes related to hydrolysate-tolerance

Ouyang, Shuiping; Zou, Lihua; Qiao, Hui; Shi, Jinjie; Zheng, Zhaojuan; Ouyang, Jia

doi:10.1016/j.biortech.2020.123855

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…The analysis was conducted using a column of Aminex HPX-87H (Bio-Rad, USA) operated at 55 °C and the eluent ow rate was 0.6 mL/min with 5 mM H 2 SO 4 , unless otherwise stated. The concentrations of phenolic inhibitors were determined as previously reported by Ouyang et al [34]. The cell growth was monitored turbidimetrically at optical density of 600 nm (OD 600 ).…”

Section: Analytic Methodsmentioning

confidence: 99%

“…The production of lactic acid from the pretreated wheat straw hydrolysate by an adapted B. coagulans was reported. 35.5 g of lactic acid was obtained from 80 g wheat straw with a yield of 70.9% [34]. Besides, to reduce processing costs as well as simpli cation of the process, one genetically engineered Bacillus subtilis capable of both degrading the cellulose and producing lactic acid was developed.…”

Section: Comparison Of Lactic Acid Production From Lignocellulosic Biomass With Other Studiesmentioning

confidence: 99%

“…The samples were withdrawn at regular intervals for determining the amount of residual sugars and lactic acid produced during the reaction. The hydrolysate-containing medium used for B. coagulans fermentation was prepared as follows: concentrated undetoxi ed corn stover dilute-acid pretreatment hydrolysate was neutralized (pH 6.5), then the hydrolysate was added to the fermentation medium (Ouyang et al [34]) of B. coagulans deprived of carbon source.…”

Section: Plasmid and Strain Constructionsmentioning

confidence: 99%

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Efficient Lactic Acid Production from Dilute Acid Pretreated Lignocellulosic Biomass by a Synthetic Consortia of Engineered Pseudomonas Putida and Bacillus Coagulans

Zou

Ouyang

et al. 2021

Preprint

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Background Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production a range of biochemicals, and a pretreatment is generally regarded to be indispensable for its bio-refinery. Nevertheless, various inhibitors that severely hindered the growth and fermentation of microorganisms were produced inevitably during the pretreatment of lignocellulose. Presently, a single microorganism that can tolerate toxic mixtures of pretreatment hydrolysate while effectively transforming sugar components into valuable compound is less well reported. Alternatively, microbial co-culture provides a simpler and more efficacious way to realize this goal via distributing metabolic tasks among proper strains. Results In this study, a novel synthetic microbial consortia, which is composed of a responsible for detoxification bacterium engineered Pseudomonas putida KT2440 and a lactic acid production specialist Bacillus coagulans NL01, was developed to directly produce lactic acid from high-toxic lignocellulosic hydrolysate. The engineered P. putida with deletion of sugar metabolism pathway was suggested to be unable to consume the major fermentable sugars of lignocellulosic hydrolysate, but can rapidly remove inhibitors in hydrolysate. With detoxification using engineered P. putida for 24 h, the pretreated hydrolysate was fermented into 35.8 g/L of lactic acid by B. coagulans with a yield of 90%. The fermentation performance of microbial co-culture was significantly improved than that single culture of B. coagulans without lactic acid production. Conclusions The microbial coculture system constructed by this study demonstrated strong potential of the process for biosynthesis of valuable product from lignocellulosic hydrolysate containing high concentration of complex inhibitors by specifically recruited consortia of robust microorganisms with desirable characteristics and also provided a feasible and attractive method for bioconversion of lignocellulosic biomass to other value-added biochemicals.

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Section: Analytic Methodsmentioning

confidence: 99%

Section: Comparison Of Lactic Acid Production From Lignocellulosic Biomass With Other Studiesmentioning

confidence: 99%

Section: Plasmid and Strain Constructionsmentioning

confidence: 99%

See 1 more Smart Citation

Efficient Lactic Acid Production from Dilute Acid Pretreated Lignocellulosic Biomass by a Synthetic Consortia of Engineered Pseudomonas Putida and Bacillus Coagulans

Zou

Ouyang

et al. 2021

Preprint

Self Cite

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“…Pretreatment of lignocellulosic biomass has been reviewed recently by Sankaran et al (2020). Pretreatment approaches aiming at LA fermentation includes acid hydrolysis (Hoheneder et al, 2021;Ouyang et al, 2020) and ionic liquid treatment (Yadav et al, 2021) (Table 3). The acid hydrolysis has the advantage of making enzymatic hydrolysis avoidable due to hydrolysis of cellulose and hemicellulose into monomeric sugars.…”

Section: Pretreatment Of Lignocellulosic Biowastementioning

confidence: 99%

“…The cellulosic wastes tested for fermentation has been achieved from sources such as sulphite liquor derived from wood pulp production (Hoheneder et al, 2021), wheat straw acid hydrolysate (Ouyang et al, 2020) and rice straw treated with ionic liquids (Yadav et al, 2021). These biomasses all consist of lignocellulose which were pre-treated and hydrolyzed into the fermentable sugars glucose, xylose, arabinose and mannose.…”

Section: Lignocellulosic Biomass and Derived Extractivesmentioning

confidence: 99%